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Highly Virulent Non-O157 Enterohemorrhagic Escherichia coli (EHEC) Serotypes Reflect Similar Phylogenetic Lineages, Providing New Insights into the Evolution of EHEC.
Appl Environ Microbiol. 2015 Oct; 81(20):7041-7.AE

Abstract

Enterohemorrhagic Escherichia coli (EHEC) is the causative agent of bloody diarrhea and extraintestinal sequelae in humans, most importantly hemolytic-uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (TTP). Besides the bacteriophage-encoded Shiga toxin gene (stx), EHEC harbors the locus of enterocyte effacement (LEE), which confers the ability to cause attaching and effacing lesions. Currently, the vast majority of EHEC infections are caused by strains belonging to five O serogroups (the "big five"), which, in addition to O157, the most important, comprise O26, O103, O111, and O145. We hypothesize that these four non-O157 EHEC serotypes differ in their phylogenies. To test this hypothesis, we used multilocus sequence typing (MLST) to analyze a large collection of 250 isolates of these four O serogroups, which were isolated from diseased as well as healthy humans and cattle between 1952 and 2009. The majority of the EHEC isolates of O serogroups O26 and O111 clustered into one sequence type complex, STC29. Isolates of O103 clustered mainly in STC20, and most isolates of O145 were found within STC32. In addition to these EHEC strains, STC29 also included stx-negative E. coli strains, termed atypical enteropathogenic E. coli (aEPEC), yet another intestinal pathogenic E. coli group. The finding that aEPEC and EHEC isolates of non-O157 O serogroups share the same phylogeny suggests an ongoing microevolutionary scenario in which the phage-encoded Shiga toxin gene stx is transferred between aEPEC and EHEC. As a consequence, aEPEC strains of STC29 can be regarded as post- or pre-EHEC isolates. Therefore, STC29 incorporates phylogenetic information useful for unraveling the evolution of EHEC.

Authors+Show Affiliations

Institute of Microbiology and Epizootics, Freie Universität Berlin, Centre for Infection Medicine, Berlin, Germany.Institute of Microbiology and Epizootics, Freie Universität Berlin, Centre for Infection Medicine, Berlin, Germany.Institute of Microbiology and Epizootics, Freie Universität Berlin, Centre for Infection Medicine, Berlin, Germany Robert Koch-Institut, Berlin, Germany.Institute of Microbiology and Epizootics, Freie Universität Berlin, Centre for Infection Medicine, Berlin, Germany.Institute of Hygiene, University Hospital Muenster, Muenster, Germany.Department of Periodontology, University of Muenster, Muenster, Germany.Department of Bacteriology, Animal and Plant Health Agency, Weybridge, Addlestone, Surrey, United Kingdom.Department of Food Microbiology and Hygiene, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.Robert Koch-Institut, Berlin, Germany.Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany.Max von Pettenkofer-Institut, Ludwig-Maximilians-Universität, Munich, Germany.Institute for Medical Microbiology and Hospital Epidemiology, Hannover Medical School, Hannover, Germany.Institute of Hygiene, University Hospital Muenster, Muenster, Germany.Institute of Microbiology and Epizootics, Freie Universität Berlin, Centre for Infection Medicine, Berlin, Germany Robert Koch-Institut, Berlin, Germany WielerLH@rki.de.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26231647

Citation

Eichhorn, Inga, et al. "Highly Virulent Non-O157 Enterohemorrhagic Escherichia Coli (EHEC) Serotypes Reflect Similar Phylogenetic Lineages, Providing New Insights Into the Evolution of EHEC." Applied and Environmental Microbiology, vol. 81, no. 20, 2015, pp. 7041-7.
Eichhorn I, Heidemanns K, Semmler T, et al. Highly Virulent Non-O157 Enterohemorrhagic Escherichia coli (EHEC) Serotypes Reflect Similar Phylogenetic Lineages, Providing New Insights into the Evolution of EHEC. Appl Environ Microbiol. 2015;81(20):7041-7.
Eichhorn, I., Heidemanns, K., Semmler, T., Kinnemann, B., Mellmann, A., Harmsen, D., Anjum, M. F., Schmidt, H., Fruth, A., Valentin-Weigand, P., Heesemann, J., Suerbaum, S., Karch, H., & Wieler, L. H. (2015). Highly Virulent Non-O157 Enterohemorrhagic Escherichia coli (EHEC) Serotypes Reflect Similar Phylogenetic Lineages, Providing New Insights into the Evolution of EHEC. Applied and Environmental Microbiology, 81(20), 7041-7. https://doi.org/10.1128/AEM.01921-15
Eichhorn I, et al. Highly Virulent Non-O157 Enterohemorrhagic Escherichia Coli (EHEC) Serotypes Reflect Similar Phylogenetic Lineages, Providing New Insights Into the Evolution of EHEC. Appl Environ Microbiol. 2015;81(20):7041-7. PubMed PMID: 26231647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly Virulent Non-O157 Enterohemorrhagic Escherichia coli (EHEC) Serotypes Reflect Similar Phylogenetic Lineages, Providing New Insights into the Evolution of EHEC. AU - Eichhorn,Inga, AU - Heidemanns,Katrin, AU - Semmler,Torsten, AU - Kinnemann,Bianca, AU - Mellmann,Alexander, AU - Harmsen,Dag, AU - Anjum,Muna F, AU - Schmidt,Herbert, AU - Fruth,Angelika, AU - Valentin-Weigand,Peter, AU - Heesemann,Jürgen, AU - Suerbaum,Sebastian, AU - Karch,Helge, AU - Wieler,Lothar H, Y1 - 2015/07/31/ PY - 2015/06/09/received PY - 2015/07/25/accepted PY - 2015/8/2/entrez PY - 2015/8/2/pubmed PY - 2016/6/14/medline SP - 7041 EP - 7 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 81 IS - 20 N2 - Enterohemorrhagic Escherichia coli (EHEC) is the causative agent of bloody diarrhea and extraintestinal sequelae in humans, most importantly hemolytic-uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (TTP). Besides the bacteriophage-encoded Shiga toxin gene (stx), EHEC harbors the locus of enterocyte effacement (LEE), which confers the ability to cause attaching and effacing lesions. Currently, the vast majority of EHEC infections are caused by strains belonging to five O serogroups (the "big five"), which, in addition to O157, the most important, comprise O26, O103, O111, and O145. We hypothesize that these four non-O157 EHEC serotypes differ in their phylogenies. To test this hypothesis, we used multilocus sequence typing (MLST) to analyze a large collection of 250 isolates of these four O serogroups, which were isolated from diseased as well as healthy humans and cattle between 1952 and 2009. The majority of the EHEC isolates of O serogroups O26 and O111 clustered into one sequence type complex, STC29. Isolates of O103 clustered mainly in STC20, and most isolates of O145 were found within STC32. In addition to these EHEC strains, STC29 also included stx-negative E. coli strains, termed atypical enteropathogenic E. coli (aEPEC), yet another intestinal pathogenic E. coli group. The finding that aEPEC and EHEC isolates of non-O157 O serogroups share the same phylogeny suggests an ongoing microevolutionary scenario in which the phage-encoded Shiga toxin gene stx is transferred between aEPEC and EHEC. As a consequence, aEPEC strains of STC29 can be regarded as post- or pre-EHEC isolates. Therefore, STC29 incorporates phylogenetic information useful for unraveling the evolution of EHEC. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/26231647/Highly_Virulent_Non_O157_Enterohemorrhagic_Escherichia_coli__EHEC__Serotypes_Reflect_Similar_Phylogenetic_Lineages_Providing_New_Insights_into_the_Evolution_of_EHEC_ L2 - http://aem.asm.org/lookup/pmidlookup?view=long&pmid=26231647 DB - PRIME DP - Unbound Medicine ER -