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Changes in metabolites, antioxidant system, and gene expression in Microcystis aeruginosa under sodium chloride stress.
Ecotoxicol Environ Saf. 2015 Dec; 122:126-35.EE

Abstract

Microcystis (M.) aeruginosa, one of the most common bloom-forming cyanobacteria, occurs worldwide. The Qingcaosha (QCS) Reservoir is undergoing eutrophication and faces the problem of saltwater intrusion. The aim of this study was to investigate the effects of sudden salinity changes on physiological parameters and related gene transcription in M. aeruginosa under controlled laboratory conditions. The results showed that sodium chloride (50, 200 and 500 mg L(-1) NaCl) inhibited the algal growth and decreased pigment concentrations (chlorophyll a, carotenoid and phycocyanin). Sodium chloride increased both the intracellular and extracellular microcystin contents and elevated the mcyD transcript level in M. aeruginosa. It also increased the malondialdehyde (MDA) content and caused cytomembrane damage. This damage caused the release of intracellular toxins into the culture medium. In addition, NaCl decreased the maximum electron transport rate, increased the levels of reactive oxygen species (ROS) and changed the cellular redox status. Consequently, NaCl inhibited the expression of cpcB, psbA and rbcL. Furthermore, NaCl increased the activities of superoxide dismutases (SOD), catalase (CAT), glutathione reductase (GR), and total glutathione peroxidase (GPx). The transcript levels of sod and reduced glutathione (gsh) were also increased after exposure to NaCl. Our results indicate that a sudden increase in salinity increases the production and excretion of microcystin, changes the cellular redox status, enhances the activities of antioxidant enzymes, inhibits photosynthesis, and affects transcript levels of related genes in M. aeruginosa.

Authors+Show Affiliations

School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China.School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China.School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China.School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China; Institute of Energy Conversion, Jiangxi Academy of Sciences, Nanchang 330096, PR China.School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China. Electronic address: ylhe@sjtu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26232039

Citation

Chen, Lei, et al. "Changes in Metabolites, Antioxidant System, and Gene Expression in Microcystis Aeruginosa Under Sodium Chloride Stress." Ecotoxicology and Environmental Safety, vol. 122, 2015, pp. 126-35.
Chen L, Mao F, Kirumba GC, et al. Changes in metabolites, antioxidant system, and gene expression in Microcystis aeruginosa under sodium chloride stress. Ecotoxicol Environ Saf. 2015;122:126-35.
Chen, L., Mao, F., Kirumba, G. C., Jiang, C., Manefield, M., & He, Y. (2015). Changes in metabolites, antioxidant system, and gene expression in Microcystis aeruginosa under sodium chloride stress. Ecotoxicology and Environmental Safety, 122, 126-35. https://doi.org/10.1016/j.ecoenv.2015.07.011
Chen L, et al. Changes in Metabolites, Antioxidant System, and Gene Expression in Microcystis Aeruginosa Under Sodium Chloride Stress. Ecotoxicol Environ Saf. 2015;122:126-35. PubMed PMID: 26232039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in metabolites, antioxidant system, and gene expression in Microcystis aeruginosa under sodium chloride stress. AU - Chen,Lei, AU - Mao,Feijian, AU - Kirumba,George Chira, AU - Jiang,Cheng, AU - Manefield,Mike, AU - He,Yiliang, Y1 - 2015/07/29/ PY - 2015/03/03/received PY - 2015/07/06/revised PY - 2015/07/07/accepted PY - 2015/8/2/entrez PY - 2015/8/2/pubmed PY - 2016/5/28/medline KW - Enzyme assay KW - Gene expression KW - Microcystin KW - Microcystis aeruginosa KW - Sodium chloride SP - 126 EP - 35 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 122 N2 - Microcystis (M.) aeruginosa, one of the most common bloom-forming cyanobacteria, occurs worldwide. The Qingcaosha (QCS) Reservoir is undergoing eutrophication and faces the problem of saltwater intrusion. The aim of this study was to investigate the effects of sudden salinity changes on physiological parameters and related gene transcription in M. aeruginosa under controlled laboratory conditions. The results showed that sodium chloride (50, 200 and 500 mg L(-1) NaCl) inhibited the algal growth and decreased pigment concentrations (chlorophyll a, carotenoid and phycocyanin). Sodium chloride increased both the intracellular and extracellular microcystin contents and elevated the mcyD transcript level in M. aeruginosa. It also increased the malondialdehyde (MDA) content and caused cytomembrane damage. This damage caused the release of intracellular toxins into the culture medium. In addition, NaCl decreased the maximum electron transport rate, increased the levels of reactive oxygen species (ROS) and changed the cellular redox status. Consequently, NaCl inhibited the expression of cpcB, psbA and rbcL. Furthermore, NaCl increased the activities of superoxide dismutases (SOD), catalase (CAT), glutathione reductase (GR), and total glutathione peroxidase (GPx). The transcript levels of sod and reduced glutathione (gsh) were also increased after exposure to NaCl. Our results indicate that a sudden increase in salinity increases the production and excretion of microcystin, changes the cellular redox status, enhances the activities of antioxidant enzymes, inhibits photosynthesis, and affects transcript levels of related genes in M. aeruginosa. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/26232039/Changes_in_metabolites_antioxidant_system_and_gene_expression_in_Microcystis_aeruginosa_under_sodium_chloride_stress_ DB - PRIME DP - Unbound Medicine ER -