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Molecular Cytogenetic Identification of a New Wheat-Rye 6R Chromosome Disomic Addition Line with Powdery Mildew Resistance.
PLoS One. 2015; 10(8):e0134534.Plos

Abstract

Rye (Secale cereale L.) possesses many valuable genes that can be used for improving disease resistance, yield and environment adaptation of wheat (Triticum aestivum L.). However, the documented resistance stocks derived from rye is faced severe challenge due to the variation of virulent isolates in the pathogen populations. Therefore, it is necessary to develop desirable germplasm and search for novel resistance gene sources against constantly accumulated variation of the virulent isolates. In the present study, a new wheat-rye line designated as WR49-1 was produced through distant hybridization and chromosome engineering protocols between common wheat cultivar Xiaoyan 6 and rye cultivar German White. Using sequential GISH (genomic in situ hybridization), mc-FISH (multicolor fluorescence in situ hybridization), mc-GISH (multicolor GISH) and EST (expressed sequence tag)-based marker analysis, WR49-1 was proved to be a new wheat-rye 6R disomic addition line. As expected, WR49-1 showed high levels of resistance to wheat powdery mildew (Blumeria graminis f. sp. tritici, Bgt) pathogens prevalent in China at the adult growth stage and 19 of 23 Bgt isolates tested at the seedling stage. According to its reaction pattern to different Bgt isolates, WR49-1 may possess new resistance gene(s) for powdery mildew, which differed from the documented powdery mildew gene, including Pm20 on chromosome arm 6RL of rye. Additionally, WR49-1 was cytologically stable, had improved agronomic characteristics and therefore could serve as an important bridge for wheat breeding and chromosome engineering.

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Authors+Show Affiliations

Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China.

The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China.

Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China.

Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China.

The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.

The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China.

Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China.

Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China.

The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

MeSH

AscomycotaChromosome MappingCytogeneticsDisease ResistanceGenes, PlantMycosesPlant BreedingPlant DiseasesSecaleTriticum

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26237413

Citation

An, Diaoguo, et al. "Molecular Cytogenetic Identification of a New Wheat-Rye 6R Chromosome Disomic Addition Line With Powdery Mildew Resistance." PloS One, vol. 10, no. 8, 2015, pp. e0134534.

An D, Zheng Q, Luo Q, et al. Molecular Cytogenetic Identification of a New Wheat-Rye 6R Chromosome Disomic Addition Line with Powdery Mildew Resistance. PLoS One. 2015;10(8):e0134534.

An, D., Zheng, Q., Luo, Q., Ma, P., Zhang, H., Li, L., Han, F., Xu, H., Xu, Y., Zhang, X., & Zhou, Y. (2015). Molecular Cytogenetic Identification of a New Wheat-Rye 6R Chromosome Disomic Addition Line with Powdery Mildew Resistance. PloS One, 10(8), e0134534. https://doi.org/10.1371/journal.pone.0134534

An D, et al. Molecular Cytogenetic Identification of a New Wheat-Rye 6R Chromosome Disomic Addition Line With Powdery Mildew Resistance. PLoS One. 2015;10(8):e0134534. PubMed PMID: 26237413.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Molecular Cytogenetic Identification of a New Wheat-Rye 6R Chromosome Disomic Addition Line with Powdery Mildew Resistance. AU - An,Diaoguo, AU - Zheng,Qi, AU - Luo,Qiaoling, AU - Ma,Pengtao, AU - Zhang,Hongxia, AU - Li,Lihui, AU - Han,Fangpu, AU - Xu,Hongxing, AU - Xu,Yunfeng, AU - Zhang,Xiaotian, AU - Zhou,Yilin, Y1 - 2015/08/03/ PY - 2015/05/07/received PY - 2015/07/09/accepted PY - 2015/8/4/entrez PY - 2015/8/4/pubmed PY - 2016/5/6/medline SP - e0134534 EP - e0134534 JF - PloS one JO - PLoS One VL - 10 IS - 8 N2 - Rye (Secale cereale L.) possesses many valuable genes that can be used for improving disease resistance, yield and environment adaptation of wheat (Triticum aestivum L.). However, the documented resistance stocks derived from rye is faced severe challenge due to the variation of virulent isolates in the pathogen populations. Therefore, it is necessary to develop desirable germplasm and search for novel resistance gene sources against constantly accumulated variation of the virulent isolates. In the present study, a new wheat-rye line designated as WR49-1 was produced through distant hybridization and chromosome engineering protocols between common wheat cultivar Xiaoyan 6 and rye cultivar German White. Using sequential GISH (genomic in situ hybridization), mc-FISH (multicolor fluorescence in situ hybridization), mc-GISH (multicolor GISH) and EST (expressed sequence tag)-based marker analysis, WR49-1 was proved to be a new wheat-rye 6R disomic addition line. As expected, WR49-1 showed high levels of resistance to wheat powdery mildew (Blumeria graminis f. sp. tritici, Bgt) pathogens prevalent in China at the adult growth stage and 19 of 23 Bgt isolates tested at the seedling stage. According to its reaction pattern to different Bgt isolates, WR49-1 may possess new resistance gene(s) for powdery mildew, which differed from the documented powdery mildew gene, including Pm20 on chromosome arm 6RL of rye. Additionally, WR49-1 was cytologically stable, had improved agronomic characteristics and therefore could serve as an important bridge for wheat breeding and chromosome engineering. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26237413/Molecular_Cytogenetic_Identification_of_a_New_Wheat_Rye_6R_Chromosome_Disomic_Addition_Line_with_Powdery_Mildew_Resistance_ DB - PRIME DP - Unbound Medicine ER -