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[Effects of Acupuncture Stimulation on Dynamic Changes of Cerebral TNF-α and C-reaction Protein Levels in Cerebral Ischemia-reperfusion Rats].
Zhen Ci Yan Jiu. 2015 Jun; 40(3):215-8.ZC

Abstract

OBJECTIVE

To observe the influence of "Tong Du Tiao Shen" (Governor-Vessel-Dredging and Mental Activity Regulating) acupuncture method on dynamic changes of cerebral TNF-α and C-reaction protein (CRP) levels in focal cerebral ischemia-reperfusion (CI/R) rats, so as to master the optimal time point of acupuncture therapy for cerebral infarction. Methods A total of 72 adult male Wistar rats were randomly divided into blank control, sham-operation, model and acupuncture groups which were further divided into 6 h, 24 h and 48 h subgroups (n 6 in each subgroup) . The CI/R model was established by occlusion of the middle cerebral artery. Acupuncture stimulation of "Baihui" (GV 20), "Fengfu" (GV 16) and "Dazhui" (GV 14) was conducted for 30 min. Neurological deficit symptoms were assessed with the Zea-Longa neurological deficit score and TNF-α and CRP contents in the brain tissue were detected using double sandwich ABC-ELISA method.

RESULTS

In comparison with the model group, the Zea-Longa neurological deficit score was significantly decreased in the acupuncture group (P<0. 01). Cerebral TNF-α and CRP levels at time-points of 6 h, 24 h and 48 h after CI/R were significantly increased in the model group than in both control and sham groups (P<0. 01). In the acupuncture group, cerebral TNF-α and CRP levels were obviously lower than those of the model group (P<0. 01).

CONCLUSION

Manual acupuncture stimulation of GV 20, GV 16 and GV 14 can relieve neurological deficit function in CI/R rats, which may be related to its function in suppressing TNF-α and CRP levels.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26237973

Citation

Wu, Hai-yang, et al. "[Effects of Acupuncture Stimulation On Dynamic Changes of Cerebral TNF-α and C-reaction Protein Levels in Cerebral Ischemia-reperfusion Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 40, no. 3, 2015, pp. 215-8.
Wu HY, Wang Y, Han W. [Effects of Acupuncture Stimulation on Dynamic Changes of Cerebral TNF-α and C-reaction Protein Levels in Cerebral Ischemia-reperfusion Rats]. Zhen Ci Yan Jiu. 2015;40(3):215-8.
Wu, H. Y., Wang, Y., & Han, W. (2015). [Effects of Acupuncture Stimulation on Dynamic Changes of Cerebral TNF-α and C-reaction Protein Levels in Cerebral Ischemia-reperfusion Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 40(3), 215-8.
Wu HY, Wang Y, Han W. [Effects of Acupuncture Stimulation On Dynamic Changes of Cerebral TNF-α and C-reaction Protein Levels in Cerebral Ischemia-reperfusion Rats]. Zhen Ci Yan Jiu. 2015;40(3):215-8. PubMed PMID: 26237973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of Acupuncture Stimulation on Dynamic Changes of Cerebral TNF-α and C-reaction Protein Levels in Cerebral Ischemia-reperfusion Rats]. AU - Wu,Hai-yang, AU - Wang,Ying, AU - Han,Wei, PY - 2015/8/5/entrez PY - 2015/8/5/pubmed PY - 2015/8/15/medline SP - 215 EP - 8 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 40 IS - 3 N2 - OBJECTIVE: To observe the influence of "Tong Du Tiao Shen" (Governor-Vessel-Dredging and Mental Activity Regulating) acupuncture method on dynamic changes of cerebral TNF-α and C-reaction protein (CRP) levels in focal cerebral ischemia-reperfusion (CI/R) rats, so as to master the optimal time point of acupuncture therapy for cerebral infarction. Methods A total of 72 adult male Wistar rats were randomly divided into blank control, sham-operation, model and acupuncture groups which were further divided into 6 h, 24 h and 48 h subgroups (n 6 in each subgroup) . The CI/R model was established by occlusion of the middle cerebral artery. Acupuncture stimulation of "Baihui" (GV 20), "Fengfu" (GV 16) and "Dazhui" (GV 14) was conducted for 30 min. Neurological deficit symptoms were assessed with the Zea-Longa neurological deficit score and TNF-α and CRP contents in the brain tissue were detected using double sandwich ABC-ELISA method. RESULTS: In comparison with the model group, the Zea-Longa neurological deficit score was significantly decreased in the acupuncture group (P<0. 01). Cerebral TNF-α and CRP levels at time-points of 6 h, 24 h and 48 h after CI/R were significantly increased in the model group than in both control and sham groups (P<0. 01). In the acupuncture group, cerebral TNF-α and CRP levels were obviously lower than those of the model group (P<0. 01). CONCLUSION: Manual acupuncture stimulation of GV 20, GV 16 and GV 14 can relieve neurological deficit function in CI/R rats, which may be related to its function in suppressing TNF-α and CRP levels. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/26237973/[Effects_of_Acupuncture_Stimulation_on_Dynamic_Changes_of_Cerebral_TNF_α_and_C_reaction_Protein_Levels_in_Cerebral_Ischemia_reperfusion_Rats]_ L2 - https://medlineplus.gov/acupuncture.html DB - PRIME DP - Unbound Medicine ER -