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Highly Sensitive Electrochemical Biosensor for Evaluation of Oxidative Stress Based on the Nanointerface of Graphene Nanocomposites Blended with Gold, Fe3O4, and Platinum Nanoparticles.
ACS Appl Mater Interfaces. 2015 Aug 26; 7(33):18441-9.AA

Abstract

High levels of H2O2 pertain to high oxidative stress and are associated with cancer, autoimmune, and neurodegenerative disease, and other related diseases. In this study, a sensitive H2O2 biosensor for evaluation of oxidative stress was fabricated on the basis of the reduced graphene oxide (RGO) nanocomposites decorated with Au, Fe3O4, and Pt nanoparticles (RGO/AuFe3O4/Pt) modified glassy carbon electrode (GCE) and used to detect the released H2O2 from cancer cells and assess the oxidative stress elicited from H2O2 in living cells. Electrochemical behavior of RGO/AuFe3O4/Pt nanocomposites exhibits excellent catalytic activity toward the relevant reduction with high selection and sensitivity, low overpotential of 0 V, low detection limit of ∼0.1 μM, large linear range from 0.5 μM to 11.5 mM, and outstanding reproducibility. The as-prepared biosensor was applied in the measurement of efflux of H2O2 from living cells including healthy normal cells and tumor cells under the external stimulation. The results display that this new nanocomposites-based biosensor is a promising candidate of nonenzymatic H2O2 sensor which has the possibility of application in clinical diagnostics to assess oxidative stress of different kinds of living cells.

Authors+Show Affiliations

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26238430

Citation

Wang, Le, et al. "Highly Sensitive Electrochemical Biosensor for Evaluation of Oxidative Stress Based On the Nanointerface of Graphene Nanocomposites Blended With Gold, Fe3O4, and Platinum Nanoparticles." ACS Applied Materials & Interfaces, vol. 7, no. 33, 2015, pp. 18441-9.
Wang L, Zhang Y, Cheng C, et al. Highly Sensitive Electrochemical Biosensor for Evaluation of Oxidative Stress Based on the Nanointerface of Graphene Nanocomposites Blended with Gold, Fe3O4, and Platinum Nanoparticles. ACS Appl Mater Interfaces. 2015;7(33):18441-9.
Wang, L., Zhang, Y., Cheng, C., Liu, X., Jiang, H., & Wang, X. (2015). Highly Sensitive Electrochemical Biosensor for Evaluation of Oxidative Stress Based on the Nanointerface of Graphene Nanocomposites Blended with Gold, Fe3O4, and Platinum Nanoparticles. ACS Applied Materials & Interfaces, 7(33), 18441-9. https://doi.org/10.1021/acsami.5b04553
Wang L, et al. Highly Sensitive Electrochemical Biosensor for Evaluation of Oxidative Stress Based On the Nanointerface of Graphene Nanocomposites Blended With Gold, Fe3O4, and Platinum Nanoparticles. ACS Appl Mater Interfaces. 2015 Aug 26;7(33):18441-9. PubMed PMID: 26238430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly Sensitive Electrochemical Biosensor for Evaluation of Oxidative Stress Based on the Nanointerface of Graphene Nanocomposites Blended with Gold, Fe3O4, and Platinum Nanoparticles. AU - Wang,Le, AU - Zhang,Yuanyuan, AU - Cheng,Chuansheng, AU - Liu,Xiaoli, AU - Jiang,Hui, AU - Wang,Xuemei, Y1 - 2015/08/13/ PY - 2015/8/5/entrez PY - 2015/8/5/pubmed PY - 2016/7/20/medline KW - Pt nanoparticles KW - electrochemical detection KW - ferroferric oxide nanoparticles KW - gold nanoparticles KW - graphene nanosheets KW - hydrogen peroxide KW - living cell SP - 18441 EP - 9 JF - ACS applied materials & interfaces JO - ACS Appl Mater Interfaces VL - 7 IS - 33 N2 - High levels of H2O2 pertain to high oxidative stress and are associated with cancer, autoimmune, and neurodegenerative disease, and other related diseases. In this study, a sensitive H2O2 biosensor for evaluation of oxidative stress was fabricated on the basis of the reduced graphene oxide (RGO) nanocomposites decorated with Au, Fe3O4, and Pt nanoparticles (RGO/AuFe3O4/Pt) modified glassy carbon electrode (GCE) and used to detect the released H2O2 from cancer cells and assess the oxidative stress elicited from H2O2 in living cells. Electrochemical behavior of RGO/AuFe3O4/Pt nanocomposites exhibits excellent catalytic activity toward the relevant reduction with high selection and sensitivity, low overpotential of 0 V, low detection limit of ∼0.1 μM, large linear range from 0.5 μM to 11.5 mM, and outstanding reproducibility. The as-prepared biosensor was applied in the measurement of efflux of H2O2 from living cells including healthy normal cells and tumor cells under the external stimulation. The results display that this new nanocomposites-based biosensor is a promising candidate of nonenzymatic H2O2 sensor which has the possibility of application in clinical diagnostics to assess oxidative stress of different kinds of living cells. SN - 1944-8252 UR - https://www.unboundmedicine.com/medline/citation/26238430/Highly_Sensitive_Electrochemical_Biosensor_for_Evaluation_of_Oxidative_Stress_Based_on_the_Nanointerface_of_Graphene_Nanocomposites_Blended_with_Gold_Fe3O4_and_Platinum_Nanoparticles_ L2 - https://dx.doi.org/10.1021/acsami.5b04553 DB - PRIME DP - Unbound Medicine ER -