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Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations.
Chromosoma. 2016 Mar; 125(1):163-72.C

Abstract

Thinopyrum bessarabicum (2n = 2x = 14, JJ or E(b)E(b)) is a valuable source of genes for bread wheat (2n = 6x = 42) improvement because of its salinity tolerance and disease resistance. Development of wheat-Th. bessarabicum translocation lines by backcrossing the amphiploid in the absence of the Ph1 gene (allowing intergenomic recombination) can assist its utilization in wheat improvement. In this study, six novel wheat-Th. bessarabicum translocation lines involving different chromosome segments (T4BS.4BL-4JL, T6BS.6BL-6JL, T5AS.5AL-5JL, T5DL.5DS-5JS, T2BS.2BL-2JL, and the whole arm translocation T1JS.1AL) were identified and characterized using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH). No background translocations between wheat genomes were observed. The involvement of five of the seven chromosomes and small terminal segments of Th. bessarabicum chromosome arm were important, contributing to both reduced linkage drag of the derived lines by minimizing agronomically deleterious genes from the alien species and high stability including transmission of the alien segment. All three wheat genomes were involved in the translocations with the alien chromosome, and GISH showed the Th. bessarabicum genome was more closely related to the D genome in wheat. All the introgression lines were disomic, stable, and with good morphological characters.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Patokar C
Department of Biology, University of Leicester, Leicester, LE1 7RH, UK. chetan.patokar@gmail.com.
Sepsi A
Department of Biology, University of Leicester, Leicester, LE1 7RH, UK. phh4@le.ac.uk.
Schwarzacher T
Department of Biology, University of Leicester, Leicester, LE1 7RH, UK. ts32@le.ac.uk.
Kishii M
International Maize and Wheat Improvement Center (CIMMYT), Km. 45, Carretera, México-Veracruz El Batán, CP 56237, Texcoco, Edo. de México, Mexico. M.Kishii@cgiar.org.
Heslop-Harrison JS
Department of Biology, University of Leicester, Leicester, LE1 7RH, UK. ais7@le.ac.uk.

MeSH

Chromosomes, PlantCytogenetic AnalysisGene Transfer, HorizontalIn Situ HybridizationInbreedingPoaceaeTranslocation, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26238987

Citation

Patokar, Chetan, et al. "Molecular Cytogenetic Characterization of Novel wheat-Thinopyrum Bessarabicum Recombinant Lines Carrying Intercalary Translocations." Chromosoma, vol. 125, no. 1, 2016, pp. 163-72.
Patokar C, Sepsi A, Schwarzacher T, et al. Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations. Chromosoma. 2016;125(1):163-72.
Patokar, C., Sepsi, A., Schwarzacher, T., Kishii, M., & Heslop-Harrison, J. S. (2016). Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations. Chromosoma, 125(1), 163-72. https://doi.org/10.1007/s00412-015-0537-6
Patokar C, et al. Molecular Cytogenetic Characterization of Novel wheat-Thinopyrum Bessarabicum Recombinant Lines Carrying Intercalary Translocations. Chromosoma. 2016;125(1):163-72. PubMed PMID: 26238987.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cytogenetic characterization of novel wheat-Thinopyrum bessarabicum recombinant lines carrying intercalary translocations. AU - Patokar,Chetan, AU - Sepsi,Adel, AU - Schwarzacher,Trude, AU - Kishii,Masahiro, AU - Heslop-Harrison,J S, Y1 - 2015/08/04/ PY - 2015/03/24/received PY - 2015/07/21/accepted PY - 2015/07/20/revised PY - 2015/8/5/entrez PY - 2015/8/5/pubmed PY - 2017/2/25/medline SP - 163 EP - 72 JF - Chromosoma JO - Chromosoma VL - 125 IS - 1 N2 - Thinopyrum bessarabicum (2n = 2x = 14, JJ or E(b)E(b)) is a valuable source of genes for bread wheat (2n = 6x = 42) improvement because of its salinity tolerance and disease resistance. Development of wheat-Th. bessarabicum translocation lines by backcrossing the amphiploid in the absence of the Ph1 gene (allowing intergenomic recombination) can assist its utilization in wheat improvement. In this study, six novel wheat-Th. bessarabicum translocation lines involving different chromosome segments (T4BS.4BL-4JL, T6BS.6BL-6JL, T5AS.5AL-5JL, T5DL.5DS-5JS, T2BS.2BL-2JL, and the whole arm translocation T1JS.1AL) were identified and characterized using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH). No background translocations between wheat genomes were observed. The involvement of five of the seven chromosomes and small terminal segments of Th. bessarabicum chromosome arm were important, contributing to both reduced linkage drag of the derived lines by minimizing agronomically deleterious genes from the alien species and high stability including transmission of the alien segment. All three wheat genomes were involved in the translocations with the alien chromosome, and GISH showed the Th. bessarabicum genome was more closely related to the D genome in wheat. All the introgression lines were disomic, stable, and with good morphological characters. SN - 1432-0886 UR - https://www.unboundmedicine.com/medline/citation/26238987/Molecular_cytogenetic_characterization_of_novel_wheat_Thinopyrum_bessarabicum_recombinant_lines_carrying_intercalary_translocations_ DB - PRIME DP - Unbound Medicine ER -