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An evaluation of the PowerSeq™ Auto System: A multiplex short tandem repeat marker kit compatible with massively parallel sequencing.
Forensic Sci Int Genet. 2015 Nov; 19:172-179.FS

Abstract

Capillary electrophoresis (CE) and multiplex amplification with fluorescent tagging have been routinely used for STR typing in forensic genetics. However, CE-based methods restrict the number of markers that can be multiplexed simultaneously and cannot detect any intra-repeat variations within STRs. Several studies already have indicated that massively parallel sequencing (MPS) may be another potential technology for STR typing. In this study, the prototype PowerSeq(™) Auto System (Promega) containing the 23 STR loci and amelogenin was evaluated using Illumina MiSeq. Results showed that single source complete profiles could be obtained using as little as 62 pg of input DNA. The reproducibility study showed that the profiles generated were consistent among multiple typing experiments for a given individual. The mixture study indicated that partial STR profiles of the minor contributor could be detected up to 19:1 mixture. The mock forensic casework study showed that full or partial profiles could be obtained from different types of single source and mixture samples. These studies indicate that the PowerSeq Auto System and the Illumina MiSeq can generate concordant results with current CE-based methods. In addition, MPS-based systems can facilitate mixture deconvolution with the detection of intra-repeat variations within length-based STR alleles.

Authors+Show Affiliations

Institute of Applied Genetics, Department of Molecular and Medical Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, Texas 76107, USA. Electronic address: Xiangpei.Zeng@live.unthsc.edu.Institute of Applied Genetics, Department of Molecular and Medical Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, Texas 76107, USA.Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA.Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA.Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA.Institute of Applied Genetics, Department of Molecular and Medical Genetics, University of North Texas Health Science Center, 3500 Camp Bowie Blvd., Fort Worth, Texas 76107, USA; Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26240968

Citation

Zeng, Xiangpei, et al. "An Evaluation of the PowerSeq™ Auto System: a Multiplex Short Tandem Repeat Marker Kit Compatible With Massively Parallel Sequencing." Forensic Science International. Genetics, vol. 19, 2015, pp. 172-179.
Zeng X, King J, Hermanson S, et al. An evaluation of the PowerSeq™ Auto System: A multiplex short tandem repeat marker kit compatible with massively parallel sequencing. Forensic Sci Int Genet. 2015;19:172-179.
Zeng, X., King, J., Hermanson, S., Patel, J., Storts, D. R., & Budowle, B. (2015). An evaluation of the PowerSeq™ Auto System: A multiplex short tandem repeat marker kit compatible with massively parallel sequencing. Forensic Science International. Genetics, 19, 172-179. https://doi.org/10.1016/j.fsigen.2015.07.015
Zeng X, et al. An Evaluation of the PowerSeq™ Auto System: a Multiplex Short Tandem Repeat Marker Kit Compatible With Massively Parallel Sequencing. Forensic Sci Int Genet. 2015;19:172-179. PubMed PMID: 26240968.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An evaluation of the PowerSeq™ Auto System: A multiplex short tandem repeat marker kit compatible with massively parallel sequencing. AU - Zeng,Xiangpei, AU - King,Jonathan, AU - Hermanson,Spencer, AU - Patel,Jaynish, AU - Storts,Douglas R, AU - Budowle,Bruce, Y1 - 2015/07/19/ PY - 2015/04/03/received PY - 2015/06/10/revised PY - 2015/07/13/accepted PY - 2015/8/5/entrez PY - 2015/8/5/pubmed PY - 2016/7/19/medline KW - Massively parallel sequencing (MPS) KW - MiSeq KW - Mixture KW - PowerSeq Auto System KW - Short tandem repeat (STR) KW - Single nucleotide polymorphism (SNP) SP - 172 EP - 179 JF - Forensic science international. Genetics JO - Forensic Sci Int Genet VL - 19 N2 - Capillary electrophoresis (CE) and multiplex amplification with fluorescent tagging have been routinely used for STR typing in forensic genetics. However, CE-based methods restrict the number of markers that can be multiplexed simultaneously and cannot detect any intra-repeat variations within STRs. Several studies already have indicated that massively parallel sequencing (MPS) may be another potential technology for STR typing. In this study, the prototype PowerSeq(™) Auto System (Promega) containing the 23 STR loci and amelogenin was evaluated using Illumina MiSeq. Results showed that single source complete profiles could be obtained using as little as 62 pg of input DNA. The reproducibility study showed that the profiles generated were consistent among multiple typing experiments for a given individual. The mixture study indicated that partial STR profiles of the minor contributor could be detected up to 19:1 mixture. The mock forensic casework study showed that full or partial profiles could be obtained from different types of single source and mixture samples. These studies indicate that the PowerSeq Auto System and the Illumina MiSeq can generate concordant results with current CE-based methods. In addition, MPS-based systems can facilitate mixture deconvolution with the detection of intra-repeat variations within length-based STR alleles. SN - 1878-0326 UR - https://www.unboundmedicine.com/medline/citation/26240968/An_evaluation_of_the_PowerSeq���_Auto_System:_A_multiplex_short_tandem_repeat_marker_kit_compatible_with_massively_parallel_sequencing_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1872-4973(15)30049-1 DB - PRIME DP - Unbound Medicine ER -