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Evaluation of the Specificity of Two Enzyme Immunoassays for Coccidioidomycosis by Using Sera from a Region of Endemicity and a Region of Nonendemicity.
Clin Vaccine Immunol. 2015 Oct; 22(10):1090-5.CV

Abstract

Coccidioidomycosis (CM), a serious life-threatening fungal infection endemic to arid regions of the western United States and Mexico, can be challenging to diagnose in a timely manner. Commercially developed enzyme immunoassays (EIAs) (from Meridian Biosciences and Immuno-Mycologics [IMMY]) have provided faster, simpler means for serodiagnosis; however, independent evaluations have questioned EIA specificity, particularly IgM-positive/IgG-negative results. This study was conducted to evaluate EIA specificity among persons residing in Puerto Rico (n = 534), where CM is not endemic (who were not likely to have been exposed to Coccidioides spp.), compared to blood bank donors residing in Arizona (n = 1,218), where CM is endemic. Upon comparing serum reactivity between Puerto Rico and Arizona, the Meridian EIA showed a significant difference in IgG reactivity (0.37% versus 3.6%; P < 0.001) but not IgM reactivity (3.4% versus 2.4%; P = 0.31). No IgM-/IgG-reactive sera were detected among sera from Puerto Rico, compared to 7 (0.57%) sera from Arizona. Similar results were observed using the IMMY EIA, although significantly (P = 0.03) fewer IgM-reactive sera from Arizona were observed, compared to the Meridian EIA. EIA-reactive sera were also evaluated by immunodiffusion before and after 3- to 4-fold concentration of the sera. These results demonstrate that elevated IgG EIA reactivity is present in sera from healthy individuals in regions of endemicity and that IgM EIA reactivity observed in sera from individuals residing outside regions of endemicity is most likely nonspecific. Other criteria, including clinical and microbiological evaluations, should be taken into account when interpreting results from surveillance studies and other reporting measures.

Authors+Show Affiliations

Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA mlindsley@cdc.gov.Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

26245352

Citation

Lindsley, Mark D., et al. "Evaluation of the Specificity of Two Enzyme Immunoassays for Coccidioidomycosis By Using Sera From a Region of Endemicity and a Region of Nonendemicity." Clinical and Vaccine Immunology : CVI, vol. 22, no. 10, 2015, pp. 1090-5.
Lindsley MD, Ahn Y, McCotter O, et al. Evaluation of the Specificity of Two Enzyme Immunoassays for Coccidioidomycosis by Using Sera from a Region of Endemicity and a Region of Nonendemicity. Clin Vaccine Immunol. 2015;22(10):1090-5.
Lindsley, M. D., Ahn, Y., McCotter, O., Gade, L., Hurst, S. F., Brandt, M. E., Park, B. J., & Litvintseva, A. P. (2015). Evaluation of the Specificity of Two Enzyme Immunoassays for Coccidioidomycosis by Using Sera from a Region of Endemicity and a Region of Nonendemicity. Clinical and Vaccine Immunology : CVI, 22(10), 1090-5. https://doi.org/10.1128/CVI.00375-15
Lindsley MD, et al. Evaluation of the Specificity of Two Enzyme Immunoassays for Coccidioidomycosis By Using Sera From a Region of Endemicity and a Region of Nonendemicity. Clin Vaccine Immunol. 2015;22(10):1090-5. PubMed PMID: 26245352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the Specificity of Two Enzyme Immunoassays for Coccidioidomycosis by Using Sera from a Region of Endemicity and a Region of Nonendemicity. AU - Lindsley,Mark D, AU - Ahn,YoonJi, AU - McCotter,Orion, AU - Gade,Lalitha, AU - Hurst,Steven F, AU - Brandt,Mary E, AU - Park,Benjamin J, AU - Litvintseva,Anastasia P, Y1 - 2015/08/05/ PY - 2015/06/26/received PY - 2015/07/30/accepted PY - 2015/8/7/entrez PY - 2015/8/8/pubmed PY - 2016/7/7/medline SP - 1090 EP - 5 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 22 IS - 10 N2 - Coccidioidomycosis (CM), a serious life-threatening fungal infection endemic to arid regions of the western United States and Mexico, can be challenging to diagnose in a timely manner. Commercially developed enzyme immunoassays (EIAs) (from Meridian Biosciences and Immuno-Mycologics [IMMY]) have provided faster, simpler means for serodiagnosis; however, independent evaluations have questioned EIA specificity, particularly IgM-positive/IgG-negative results. This study was conducted to evaluate EIA specificity among persons residing in Puerto Rico (n = 534), where CM is not endemic (who were not likely to have been exposed to Coccidioides spp.), compared to blood bank donors residing in Arizona (n = 1,218), where CM is endemic. Upon comparing serum reactivity between Puerto Rico and Arizona, the Meridian EIA showed a significant difference in IgG reactivity (0.37% versus 3.6%; P < 0.001) but not IgM reactivity (3.4% versus 2.4%; P = 0.31). No IgM-/IgG-reactive sera were detected among sera from Puerto Rico, compared to 7 (0.57%) sera from Arizona. Similar results were observed using the IMMY EIA, although significantly (P = 0.03) fewer IgM-reactive sera from Arizona were observed, compared to the Meridian EIA. EIA-reactive sera were also evaluated by immunodiffusion before and after 3- to 4-fold concentration of the sera. These results demonstrate that elevated IgG EIA reactivity is present in sera from healthy individuals in regions of endemicity and that IgM EIA reactivity observed in sera from individuals residing outside regions of endemicity is most likely nonspecific. Other criteria, including clinical and microbiological evaluations, should be taken into account when interpreting results from surveillance studies and other reporting measures. SN - 1556-679X UR - https://www.unboundmedicine.com/medline/citation/26245352/Evaluation_of_the_Specificity_of_Two_Enzyme_Immunoassays_for_Coccidioidomycosis_by_Using_Sera_from_a_Region_of_Endemicity_and_a_Region_of_Nonendemicity_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&amp;pmid=26245352 DB - PRIME DP - Unbound Medicine ER -