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Maternal and neonatal dietary intake of balanced n-6/n-3 fatty acids modulates experimental colitis in young adult rats.
Eur J Nutr. 2016 Aug; 55(5):1875-90.EJ

Abstract

BACKGROUND

The imbalance of n-6 and n-3 polyunsaturated fatty acids in the maternal diet impairs intestinal barrier development and sensitizes the colon response to inflammatory insults in the young rats. With a view to overcoming this issue, we designed this study to investigate the effect of maternal and neonatal intake of different proportions of n-6/n-3 fatty acids on colon inflammation in the young adult rats.

METHODS

Female Wistar rats were assigned into four groups, and each group fed one of four semisynthetic diets, namely n-6, low n-3, n-6/n-3 and n-3 fatty acids for 8 weeks prior to mating, during gestation and lactation periods. At weaning, the pups were separated from the dams and fed diet similar to the mothers. Colitis was induced on postnatal day 35, by administering 2 % dextran sulfate sodium in drinking water for 10 days. Colitis was assessed based on the clinical and inflammatory markers in the colon. Fatty acid analysis was done in liver, RBC, colon and spleen.

RESULTS

A balanced n-6/n-3 PUFA diet significantly improved the body weight loss, rectal bleeding and mortality in rats. This was associated with lower myeloperoxidase activity, nitric oxide, prostaglandin E2, TNF-α and IL-6, IL-8, COX-2 and iNOS levels in the colon tissues. Fatty acid analysis has shown that the arachidonic acid/docosahexaenoic acid ratio was significantly lower in liver, RBC, colon and spleen in n-6/n-3 and n-3 diet groups.

CONCLUSION

We demonstrate that balanced n-6/n-3 PUFA supplementation in maternal and neonatal diet alters systemic AA/DHA ratio and attenuates colon inflammation in the young adult rats.

Authors+Show Affiliations

Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, Mysore, 570020, India.Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, Mysore, 570020, India. aknaidu@cftri.res.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26246200

Citation

Reddy, K Vijay Kumar, and K Akhilender Naidu. "Maternal and Neonatal Dietary Intake of Balanced N-6/n-3 Fatty Acids Modulates Experimental Colitis in Young Adult Rats." European Journal of Nutrition, vol. 55, no. 5, 2016, pp. 1875-90.
Reddy KV, Naidu KA. Maternal and neonatal dietary intake of balanced n-6/n-3 fatty acids modulates experimental colitis in young adult rats. Eur J Nutr. 2016;55(5):1875-90.
Reddy, K. V., & Naidu, K. A. (2016). Maternal and neonatal dietary intake of balanced n-6/n-3 fatty acids modulates experimental colitis in young adult rats. European Journal of Nutrition, 55(5), 1875-90. https://doi.org/10.1007/s00394-015-1004-0
Reddy KV, Naidu KA. Maternal and Neonatal Dietary Intake of Balanced N-6/n-3 Fatty Acids Modulates Experimental Colitis in Young Adult Rats. Eur J Nutr. 2016;55(5):1875-90. PubMed PMID: 26246200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maternal and neonatal dietary intake of balanced n-6/n-3 fatty acids modulates experimental colitis in young adult rats. AU - Reddy,K Vijay Kumar, AU - Naidu,K Akhilender, Y1 - 2015/08/06/ PY - 2015/03/20/received PY - 2015/07/21/accepted PY - 2015/8/7/entrez PY - 2015/8/8/pubmed PY - 2017/4/1/medline KW - Garden cress oil KW - Inflammatory mediators KW - Maternal and neonatal diet KW - Ulcerative colitis SP - 1875 EP - 90 JF - European journal of nutrition JO - Eur J Nutr VL - 55 IS - 5 N2 - BACKGROUND: The imbalance of n-6 and n-3 polyunsaturated fatty acids in the maternal diet impairs intestinal barrier development and sensitizes the colon response to inflammatory insults in the young rats. With a view to overcoming this issue, we designed this study to investigate the effect of maternal and neonatal intake of different proportions of n-6/n-3 fatty acids on colon inflammation in the young adult rats. METHODS: Female Wistar rats were assigned into four groups, and each group fed one of four semisynthetic diets, namely n-6, low n-3, n-6/n-3 and n-3 fatty acids for 8 weeks prior to mating, during gestation and lactation periods. At weaning, the pups were separated from the dams and fed diet similar to the mothers. Colitis was induced on postnatal day 35, by administering 2 % dextran sulfate sodium in drinking water for 10 days. Colitis was assessed based on the clinical and inflammatory markers in the colon. Fatty acid analysis was done in liver, RBC, colon and spleen. RESULTS: A balanced n-6/n-3 PUFA diet significantly improved the body weight loss, rectal bleeding and mortality in rats. This was associated with lower myeloperoxidase activity, nitric oxide, prostaglandin E2, TNF-α and IL-6, IL-8, COX-2 and iNOS levels in the colon tissues. Fatty acid analysis has shown that the arachidonic acid/docosahexaenoic acid ratio was significantly lower in liver, RBC, colon and spleen in n-6/n-3 and n-3 diet groups. CONCLUSION: We demonstrate that balanced n-6/n-3 PUFA supplementation in maternal and neonatal diet alters systemic AA/DHA ratio and attenuates colon inflammation in the young adult rats. SN - 1436-6215 UR - https://www.unboundmedicine.com/medline/citation/26246200/Maternal_and_neonatal_dietary_intake_of_balanced_n_6/n_3_fatty_acids_modulates_experimental_colitis_in_young_adult_rats_ L2 - https://dx.doi.org/10.1007/s00394-015-1004-0 DB - PRIME DP - Unbound Medicine ER -