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Comparison of Scolicidal Effects of Amphotricin B, Silver Nanoparticles,_and Foeniculum vulgare Mill on Hydatid Cysts Protoscoleces.

Abstract

BACKGROUND

Surgery is the preferred treatment for hydatid cyst (cystic echinococcosis, CE). At present, various scolicidal agents have been used for inactivation of protoscoleces during surgery, but they are associated with adverse side effects. The aim of the present study was to evaluate the scolicidal effects of amphotricin B, Silver nano particles, Foeniculum vulgare Mill, essential oil and hypertonic saline against protoscoleces of hydatid cyst on an in vitro model.

METHODS

Protoscoleces were aseptically aspirated from the naturally infected livers of sheep and goats. Various concentrations of AmB (2.5-20 mg/ml), Ag-NPs (0.5-4 mg/ml), F. vulgare essential oil (0.125-1 mg/ml) and hypertonic saline (10-20%) were used for 5-60 min. Eosin exclusion test was used to determine the viability of protoscoleces.

RESULTS

Maximum protoscolicidal effect of AmB and Ag-NPs was found at concentrations of 20 and 4 mg/mL, resulting in only 82.3% and 71.6% of the protoscoleces after 60 min of incubation, respectively. In contrast, F. vulgare essential oil at concentration of 1 mg/ml and hypertonic saline 20% killed 100% protoscoleces after 5 and 10 min of exposure, respectively.

CONCLUSION

The results indicated weak scolicidal activity of AmB and Ag-NPs; whereas F. vulgare essential oil had potent scolicidal activity against protoscoleces of hydatid cyst that revealed the potential of F. vulgare as a natural source for the production of new scolicidal agent for use in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking the essential oil and its active component in the in vivo model.

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  • Authors+Show Affiliations

    ,

    Dept. of Surgery and the Research Center for Tropical and Infectious diseases, Kerman University of Medical Sciences, Kerman, Iran.

    ,

    Dept. of Surgery and the Research Center for Tropical and Infectious diseases, Kerman University of Medical Sciences, Kerman, Iran.

    ,

    Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.

    Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    26246818

    Citation

    Lashkarizadeh, Mohammad Reza, et al. "Comparison of Scolicidal Effects of Amphotricin B, Silver Nanoparticles,_and Foeniculum Vulgare Mill On Hydatid Cysts Protoscoleces." Iranian Journal of Parasitology, vol. 10, no. 2, 2015, pp. 206-12.
    Lashkarizadeh MR, Asgaripour K, Saedi Dezaki E, et al. Comparison of Scolicidal Effects of Amphotricin B, Silver Nanoparticles,_and Foeniculum vulgare Mill on Hydatid Cysts Protoscoleces. Iran J Parasitol. 2015;10(2):206-12.
    Lashkarizadeh, M. R., Asgaripour, K., Saedi Dezaki, E., & Fasihi Harandi, M. (2015). Comparison of Scolicidal Effects of Amphotricin B, Silver Nanoparticles,_and Foeniculum vulgare Mill on Hydatid Cysts Protoscoleces. Iranian Journal of Parasitology, 10(2), pp. 206-12.
    Lashkarizadeh MR, et al. Comparison of Scolicidal Effects of Amphotricin B, Silver Nanoparticles,_and Foeniculum Vulgare Mill On Hydatid Cysts Protoscoleces. Iran J Parasitol. 2015;10(2):206-12. PubMed PMID: 26246818.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Comparison of Scolicidal Effects of Amphotricin B, Silver Nanoparticles,_and Foeniculum vulgare Mill on Hydatid Cysts Protoscoleces. AU - Lashkarizadeh,Mohammad Reza, AU - Asgaripour,Keivan, AU - Saedi Dezaki,Ebrahim, AU - Fasihi Harandi,Majid, PY - 2014/11/05/received PY - 2015/02/23/accepted PY - 2015/8/7/entrez PY - 2015/8/8/pubmed PY - 2015/8/8/medline KW - Ag-Nps KW - Cystic echinococcosis KW - Fennel KW - Fungizone KW - Hypertonic saline SP - 206 EP - 12 JF - Iranian journal of parasitology JO - Iran J Parasitol VL - 10 IS - 2 N2 - BACKGROUND: Surgery is the preferred treatment for hydatid cyst (cystic echinococcosis, CE). At present, various scolicidal agents have been used for inactivation of protoscoleces during surgery, but they are associated with adverse side effects. The aim of the present study was to evaluate the scolicidal effects of amphotricin B, Silver nano particles, Foeniculum vulgare Mill, essential oil and hypertonic saline against protoscoleces of hydatid cyst on an in vitro model. METHODS: Protoscoleces were aseptically aspirated from the naturally infected livers of sheep and goats. Various concentrations of AmB (2.5-20 mg/ml), Ag-NPs (0.5-4 mg/ml), F. vulgare essential oil (0.125-1 mg/ml) and hypertonic saline (10-20%) were used for 5-60 min. Eosin exclusion test was used to determine the viability of protoscoleces. RESULTS: Maximum protoscolicidal effect of AmB and Ag-NPs was found at concentrations of 20 and 4 mg/mL, resulting in only 82.3% and 71.6% of the protoscoleces after 60 min of incubation, respectively. In contrast, F. vulgare essential oil at concentration of 1 mg/ml and hypertonic saline 20% killed 100% protoscoleces after 5 and 10 min of exposure, respectively. CONCLUSION: The results indicated weak scolicidal activity of AmB and Ag-NPs; whereas F. vulgare essential oil had potent scolicidal activity against protoscoleces of hydatid cyst that revealed the potential of F. vulgare as a natural source for the production of new scolicidal agent for use in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking the essential oil and its active component in the in vivo model. SN - 1735-7020 UR - https://www.unboundmedicine.com/medline/citation/26246818/Comparison_of_Scolicidal_Effects_of_Amphotricin_B_Silver_Nanoparticles_and_Foeniculum_vulgare_Mill_on_Hydatid_Cysts_Protoscoleces_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26246818/ DB - PRIME DP - Unbound Medicine ER -