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The Pattern of Fatty Acids Displaced by EPA and DHA Following 12 Months Supplementation Varies between Blood Cell and Plasma Fractions.
Nutrients. 2015 Aug 03; 7(8):6281-93.N

Abstract

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are increased in plasma lipids and blood cell membranes in response to supplementation. Whilst arachidonic acid (AA) is correspondingly decreased, the effect on other fatty acids (FA) is less well described and there may be site-specific differences. In response to 12 months EPA + DHA supplementation in doses equivalent to 0-4 portions of oily fish/week (1 portion: 3.27 g EPA+DHA) multinomial regression analysis was used to identify important FA changes for plasma phosphatidylcholine (PC), cholesteryl ester (CE) and triglyceride (TAG) and for blood mononuclear cells (MNC), red blood cells (RBC) and platelets (PLAT). Dose-dependent increases in EPA + DHA were matched by decreases in several n-6 polyunsaturated fatty acids (PUFA) in PC, CE, RBC and PLAT, but were predominantly compensated for by oleic acid in TAG. Changes were observed for all FA classes in MNC. Consequently the n-6:n-3 PUFA ratio was reduced in a dose-dependent manner in all pools after 12 months (37%-64% of placebo in the four portions group). We conclude that the profile of the FA decreased in exchange for the increase in EPA + DHA following supplementation differs by FA pool with implications for understanding the impact of n-3 PUFA on blood lipid and blood cell biology.

Authors+Show Affiliations

MRC Human Nutrition Research, Elsie Widdowson Laboratory, Fulbourn Road, Cambridge CB1 9NL, UK. celia.walker@mrc-hnr.cam.ac.uk.Human Development and Health Academic Unit, Faculty of Medicine, University of Southampton, Tremona Road, Southampton SO16 6YD, UK. A.West@soton.ac.uk.MRC Human Nutrition Research, Elsie Widdowson Laboratory, Fulbourn Road, Cambridge CB1 9NL, UK. lmbrowning@btinternet.com.Human Development and Health Academic Unit, Faculty of Medicine, University of Southampton, Tremona Road, Southampton SO16 6YD, UK. jm24@soton.ac.uk.MRC Human Nutrition Research, Elsie Widdowson Laboratory, Fulbourn Road, Cambridge CB1 9NL, UK. j.m.gambell@gmail.com.MRC Human Nutrition Research, Elsie Widdowson Laboratory, Fulbourn Road, Cambridge CB1 9NL, UK. susan.jebb@phc.ox.ac.uk. Nuffield Department of Primary Care Health Sciences, University of Oxford, Woodstock Road, Oxford OX2 6GG, UK. susan.jebb@phc.ox.ac.uk.Human Development and Health Academic Unit, Faculty of Medicine, University of Southampton, Tremona Road, Southampton SO16 6YD, UK. pcc@soton.ac.uk. NIHR Southampton Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust, Tremona Road, Southampton SO16 6YD, UK. pcc@soton.ac.uk. Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia. pcc@soton.ac.uk.

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26247960

Citation

Walker, Celia G., et al. "The Pattern of Fatty Acids Displaced By EPA and DHA Following 12 Months Supplementation Varies Between Blood Cell and Plasma Fractions." Nutrients, vol. 7, no. 8, 2015, pp. 6281-93.
Walker CG, West AL, Browning LM, et al. The Pattern of Fatty Acids Displaced by EPA and DHA Following 12 Months Supplementation Varies between Blood Cell and Plasma Fractions. Nutrients. 2015;7(8):6281-93.
Walker, C. G., West, A. L., Browning, L. M., Madden, J., Gambell, J. M., Jebb, S. A., & Calder, P. C. (2015). The Pattern of Fatty Acids Displaced by EPA and DHA Following 12 Months Supplementation Varies between Blood Cell and Plasma Fractions. Nutrients, 7(8), 6281-93. https://doi.org/10.3390/nu7085285
Walker CG, et al. The Pattern of Fatty Acids Displaced By EPA and DHA Following 12 Months Supplementation Varies Between Blood Cell and Plasma Fractions. Nutrients. 2015 Aug 3;7(8):6281-93. PubMed PMID: 26247960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Pattern of Fatty Acids Displaced by EPA and DHA Following 12 Months Supplementation Varies between Blood Cell and Plasma Fractions. AU - Walker,Celia G, AU - West,Annette L, AU - Browning,Lucy M, AU - Madden,Jackie, AU - Gambell,Joanna M, AU - Jebb,Susan A, AU - Calder,Philip C, Y1 - 2015/08/03/ PY - 2015/05/26/received PY - 2015/05/26/revised PY - 2015/07/27/accepted PY - 2015/8/7/entrez PY - 2015/8/8/pubmed PY - 2016/5/3/medline KW - EPA and DHA supplementation KW - blood cell fatty acids KW - fatty acid displacement KW - n-3 fatty acid KW - n-6 fatty acid KW - plasma fatty acid fractions SP - 6281 EP - 93 JF - Nutrients JO - Nutrients VL - 7 IS - 8 N2 - Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are increased in plasma lipids and blood cell membranes in response to supplementation. Whilst arachidonic acid (AA) is correspondingly decreased, the effect on other fatty acids (FA) is less well described and there may be site-specific differences. In response to 12 months EPA + DHA supplementation in doses equivalent to 0-4 portions of oily fish/week (1 portion: 3.27 g EPA+DHA) multinomial regression analysis was used to identify important FA changes for plasma phosphatidylcholine (PC), cholesteryl ester (CE) and triglyceride (TAG) and for blood mononuclear cells (MNC), red blood cells (RBC) and platelets (PLAT). Dose-dependent increases in EPA + DHA were matched by decreases in several n-6 polyunsaturated fatty acids (PUFA) in PC, CE, RBC and PLAT, but were predominantly compensated for by oleic acid in TAG. Changes were observed for all FA classes in MNC. Consequently the n-6:n-3 PUFA ratio was reduced in a dose-dependent manner in all pools after 12 months (37%-64% of placebo in the four portions group). We conclude that the profile of the FA decreased in exchange for the increase in EPA + DHA following supplementation differs by FA pool with implications for understanding the impact of n-3 PUFA on blood lipid and blood cell biology. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/26247960/The_Pattern_of_Fatty_Acids_Displaced_by_EPA_and_DHA_Following_12_Months_Supplementation_Varies_between_Blood_Cell_and_Plasma_Fractions_ L2 - https://www.mdpi.com/resolver?pii=nu7085285 DB - PRIME DP - Unbound Medicine ER -