Citation
Chen, Shan-Wen, et al. "Protective Effect of Hydrogen Sulfide On TNF-α and IFN-γ-induced Injury of Intestinal Epithelial Barrier Function in Caco-2 Monolayers." Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], vol. 64, no. 10, 2015, pp. 789-97.
Chen SW, Zhu J, Zuo S, et al. Protective effect of hydrogen sulfide on TNF-α and IFN-γ-induced injury of intestinal epithelial barrier function in Caco-2 monolayers. Inflamm Res. 2015;64(10):789-97.
Chen, S. W., Zhu, J., Zuo, S., Zhang, J. L., Chen, Z. Y., Chen, G. W., Wang, X., Pan, Y. S., Liu, Y. C., & Wang, P. Y. (2015). Protective effect of hydrogen sulfide on TNF-α and IFN-γ-induced injury of intestinal epithelial barrier function in Caco-2 monolayers. Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], 64(10), 789-97. https://doi.org/10.1007/s00011-015-0862-5
Chen SW, et al. Protective Effect of Hydrogen Sulfide On TNF-α and IFN-γ-induced Injury of Intestinal Epithelial Barrier Function in Caco-2 Monolayers. Inflamm Res. 2015;64(10):789-97. PubMed PMID: 26249853.
TY - JOUR
T1 - Protective effect of hydrogen sulfide on TNF-α and IFN-γ-induced injury of intestinal epithelial barrier function in Caco-2 monolayers.
AU - Chen,Shan-Wen,
AU - Zhu,Jing,
AU - Zuo,Shuai,
AU - Zhang,Jun-Ling,
AU - Chen,Zi-Yi,
AU - Chen,Guo-Wei,
AU - Wang,Xin,
AU - Pan,Yi-Sheng,
AU - Liu,Yu-Cun,
AU - Wang,Peng-Yuan,
Y1 - 2015/08/07/
PY - 2015/06/02/received
PY - 2015/07/30/accepted
PY - 2015/07/28/revised
PY - 2015/8/8/entrez
PY - 2015/8/8/pubmed
PY - 2016/6/15/medline
KW - H2S
KW - MLCK
KW - NF-kB
KW - Tight junction
KW - ZO-1
SP - 789
EP - 97
JF - Inflammation research : official journal of the European Histamine Research Society ... [et al.]
JO - Inflamm Res
VL - 64
IS - 10
N2 - BACKGROUND AND AIM: Studies have verified the protective effect of Hydrogen Sulfide (H2S) on gastric ulcer and ulcerative colitis, but the mechanisms are not fully illustrated. In this study, the possible protective effect of H2S on TNF-α/IFN-γ induced barrier dysfunction was investigated in Caco-2 cell monolayers. METHOD: The barrier function of Caco-2 monolayers was evaluated by measuring trans-epithelial electrical resistance (TEER) and FITC-Dextran 4 kDa (FD-4) trans-membrane flux. ZO-1 and Occludin were chosen as markers of the localization of tight junction (TJ) proteins for immunofluorescence. The expression of MLCK and phosphorylation level of myosin light chain (MLC) were measured by immunoblotting. The activation of NF-kB p65 was analyzed by EMSA and immunofluorescence. RESULTS: NaHS at 500 uM significantly attenuated TNF-α/IFN-γ-indueced Caco-2 monolayer barrier injury. The increased expression of MLCK and increased phosphorylation level of MLC induced by TNF-α/IFN-γ was also inhibited significantly by NaHS. Additionally, NaHS inhibited TNF-α/IFN-γ induced activation and nuclear translocation of NF-kB p65. CONCLUSION: The present study reveals the protective effect of H2S on TNF-α and IFN-γ-induced injury of intestinal epithelial barrier function in Caco-2 monolayers and suggests that the suppression of MLCK-P-MLC signaling mediated by NF-kB P65 might be one of the mechanisms underlying the protective effect of H2S.
SN - 1420-908X
UR - https://www.unboundmedicine.com/medline/citation/26249853/Protective_effect_of_hydrogen_sulfide_on_TNF_α_and_IFN_γ_induced_injury_of_intestinal_epithelial_barrier_function_in_Caco_2_monolayers_
L2 - https://dx.doi.org/10.1007/s00011-015-0862-5
DB - PRIME
DP - Unbound Medicine
ER -
