Electrochemical Impedance Immunosensor Based on Self-Assembled Monolayers for Rapid Detection of Escherichia coli O157:H7 with Signal Amplification Using Lectin.
Sensors (Basel). 2015 Aug 05; 15(8):19212-24.S

Abstract

Escherichia coli O157:H7 is a predominant foodborne pathogen with severe pathogenicity, leading to increasing attention given to rapid and sensitive detection. Herein, we propose an impedance biosensor using new kinds of screen-printed interdigitated microelectrodes (SPIMs) and wheat germ agglutinin (WGA) for signal amplification to detect E. coli O157:H7 with high sensitivity and time-efficiency. The SPIMs integrate the high sensitivity and short response time of the interdigitated electrodes and the low cost of the screen-printed electrodes. Self-assembling of bi-functional 3-dithiobis-(sulfosuccinimidyl-propionate) (DTSP) on the SPIMs was investigated and was proved to be able to improve adsorption quantity and stability of biomaterials. WGA was further adopted to enhance the signal taking advantage of the abundant lectin-binding sites on the bacteria surface. The immunosensor exhibited a detection limit of 102 cfu·mL(-1), with a linear detection range from 10(2) to 10(7) cfu·mL(-1) (r2 = 0.98). The total detection time was less than 1 h, showing its comparable sensitivity and rapid response. Furthermore, the low cost of one SPIM significantly reduced the detection cost of the biosensor. The biosensor may have great promise in food safety analysis and lead to a portable biosensing system for routine monitoring of foodborne pathogens.

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Authors+Show Affiliations

Li Z

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. lizhanming1018@126.com.

Fu Y

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. yingchunfu@126.com.

Fang W

College of Animal Science, Zhejiang University, Hangzhou 310058, China. whfang@zju.deu.cn.

Li Y

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. yanbinli@zju.edu.cn. Department of Biological & Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA. yanbinli@zju.edu.cn.

MeSH

Biocompatible MaterialsBiosensing TechniquesComputer SimulationElectric ImpedanceElectrochemical TechniquesEscherichia coli O157MicroelectrodesSignal Processing, Computer-AssistedSuccinimidesWheat Germ Agglutinins

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26251911

Citation

Li, Zhanming, et al. "Electrochemical Impedance Immunosensor Based On Self-Assembled Monolayers for Rapid Detection of Escherichia Coli O157:H7 With Signal Amplification Using Lectin." Sensors (Basel, Switzerland), vol. 15, no. 8, 2015, pp. 19212-24.

Li Z, Fu Y, Fang W, et al. Electrochemical Impedance Immunosensor Based on Self-Assembled Monolayers for Rapid Detection of Escherichia coli O157:H7 with Signal Amplification Using Lectin. Sensors (Basel). 2015;15(8):19212-24.

Li, Z., Fu, Y., Fang, W., & Li, Y. (2015). Electrochemical Impedance Immunosensor Based on Self-Assembled Monolayers for Rapid Detection of Escherichia coli O157:H7 with Signal Amplification Using Lectin. Sensors (Basel, Switzerland), 15(8), 19212-24. https://doi.org/10.3390/s150819212

Li Z, et al. Electrochemical Impedance Immunosensor Based On Self-Assembled Monolayers for Rapid Detection of Escherichia Coli O157:H7 With Signal Amplification Using Lectin. Sensors (Basel). 2015 Aug 5;15(8):19212-24. PubMed PMID: 26251911.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Electrochemical Impedance Immunosensor Based on Self-Assembled Monolayers for Rapid Detection of Escherichia coli O157:H7 with Signal Amplification Using Lectin. AU - Li,Zhanming, AU - Fu,Yingchun, AU - Fang,Weihuan, AU - Li,Yanbin, Y1 - 2015/08/05/ PY - 2015/06/14/received PY - 2015/07/10/revised PY - 2015/07/14/accepted PY - 2015/8/8/entrez PY - 2015/8/8/pubmed PY - 2016/2/16/medline KW - Escherichia coli O157:H7 KW - ·impedance immunosensor KW - ·lectin KW - ·screen-printed interdigitated microelectrode SP - 19212 EP - 24 JF - Sensors (Basel, Switzerland) JO - Sensors (Basel) VL - 15 IS - 8 N2 - Escherichia coli O157:H7 is a predominant foodborne pathogen with severe pathogenicity, leading to increasing attention given to rapid and sensitive detection. Herein, we propose an impedance biosensor using new kinds of screen-printed interdigitated microelectrodes (SPIMs) and wheat germ agglutinin (WGA) for signal amplification to detect E. coli O157:H7 with high sensitivity and time-efficiency. The SPIMs integrate the high sensitivity and short response time of the interdigitated electrodes and the low cost of the screen-printed electrodes. Self-assembling of bi-functional 3-dithiobis-(sulfosuccinimidyl-propionate) (DTSP) on the SPIMs was investigated and was proved to be able to improve adsorption quantity and stability of biomaterials. WGA was further adopted to enhance the signal taking advantage of the abundant lectin-binding sites on the bacteria surface. The immunosensor exhibited a detection limit of 102 cfu·mL(-1), with a linear detection range from 10(2) to 10(7) cfu·mL(-1) (r2 = 0.98). The total detection time was less than 1 h, showing its comparable sensitivity and rapid response. Furthermore, the low cost of one SPIM significantly reduced the detection cost of the biosensor. The biosensor may have great promise in food safety analysis and lead to a portable biosensing system for routine monitoring of foodborne pathogens. SN - 1424-8220 UR - https://www.unboundmedicine.com/medline/citation/26251911/Electrochemical_Impedance_Immunosensor_Based_on_Self_Assembled_Monolayers_for_Rapid_Detection_of_Escherichia_coli_O157:H7_with_Signal_Amplification_Using_Lectin_ DB - PRIME DP - Unbound Medicine ER -

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Electrochemical Impedance Immunosensor Based on Self-Assembled Monolayers for Rapid Detection of Escherichia coli O157:H7 with Signal Amplification Using Lectin.Sensors (Basel). 2015 Aug 05; 15(8):19212-24.S