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[Effect of curcumin on oligomer formation and mitochondrial ATP-sensitive potassium channels induced by overexpression or mutation of α-synuclein].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2015 Aug; 32(4):462-7.ZY

Abstract

OBJECTIVE

To investigate the effect of curcumin on oligomer formation and mitochondrial ATP-sensitive potassium channels (mitoKATP) induced by overexpression or mutation of α-synuclein.

METHODS

Recombinant plasmids α-synuclein-pEGFP-A53T and α-synuclein-pEGFP-WT were transfected into PC12 cells by lipofectamin method, and intervened by application of curcumin (20 μmol/L) and 5-hydroxydecanoate (5-HD). Oligomer formation in the cultured cells was identified by Western blotting and Dot blotting. Cytotoxicity and apoptosis of the PC12 cells were measured by lactate dehydrogenase (LDH) and JC-1 assays. mitoKATP were identified by Western blotting and whole cell patch clamp.

RESULTS

Curcumin has significantly reduced the oligomer formation induced by overexpression or mutation of α-synuclein in the cultured cells. LDH has decreased by 36.3% and 23.5%, and red/green fluorescence ratio of JC-1 was increased respectively by 48.46% and 50.33% after application of curcumin (P<0.05). Protein expression of Kir6.2 has decreased and mitoKATP channel current has significantly increased (P<0.05).

CONCLUSION

Curcumin can inhibit α-synuclein gene overexpression or mutation induced α-synuclein oligomers formation. It may block apoptosis induced by wild-type overexpression or mutation of α-synuclein. By stabilizing mitochondrial membrane potential. Opening of mitoKATP channel may have been the initiating protective mechanism of apoptosis induced by wild-type overexpression or mutation of α-synuclein. Curcumin may antagonize above cytotoxicity through further opening the mitoKATP channel.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Chen T
Department of Neurology, Hainan Provincial People's Hospital, Haikou, Hainan 570311, P. R. China. ctxwyc@163.com.
Deng Y
No affiliation info available
Liao X
No affiliation info available
Zhao J
No affiliation info available
Wen G
No affiliation info available
Weng G
No affiliation info available
Ma F
No affiliation info available
Zheng Y
No affiliation info available

MeSH

AnimalsApoptosisCell LineCurcuminHumansKATP ChannelsMitochondriaMutationPC12 CellsParkinson DiseaseRatsalpha-Synuclein

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26252085

Citation

Chen, Tao, et al. "[Effect of Curcumin On Oligomer Formation and Mitochondrial ATP-sensitive Potassium Channels Induced By Overexpression or Mutation of Α-synuclein]." Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics, vol. 32, no. 4, 2015, pp. 462-7.
Chen T, Deng Y, Liao X, et al. [Effect of curcumin on oligomer formation and mitochondrial ATP-sensitive potassium channels induced by overexpression or mutation of α-synuclein]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2015;32(4):462-7.
Chen, T., Deng, Y., Liao, X., Zhao, J., Wen, G., Weng, G., Ma, F., & Zheng, Y. (2015). [Effect of curcumin on oligomer formation and mitochondrial ATP-sensitive potassium channels induced by overexpression or mutation of α-synuclein]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics, 32(4), 462-7. https://doi.org/10.3760/cma.j.issn.1003-9406.2015.04.002
Chen T, et al. [Effect of Curcumin On Oligomer Formation and Mitochondrial ATP-sensitive Potassium Channels Induced By Overexpression or Mutation of Α-synuclein]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2015;32(4):462-7. PubMed PMID: 26252085.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of curcumin on oligomer formation and mitochondrial ATP-sensitive potassium channels induced by overexpression or mutation of α-synuclein]. AU - Chen,Tao, AU - Deng,Yidong, AU - Liao,Xiaoping, AU - Zhao,Jiannong, AU - Wen,Guoqiang, AU - Weng,Guohu, AU - Ma,Fei, AU - Zheng,Yingying, PY - 2015/8/8/entrez PY - 2015/8/8/pubmed PY - 2015/11/17/medline SP - 462 EP - 7 JF - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics JO - Zhonghua Yi Xue Yi Chuan Xue Za Zhi VL - 32 IS - 4 N2 - OBJECTIVE: To investigate the effect of curcumin on oligomer formation and mitochondrial ATP-sensitive potassium channels (mitoKATP) induced by overexpression or mutation of α-synuclein. METHODS: Recombinant plasmids α-synuclein-pEGFP-A53T and α-synuclein-pEGFP-WT were transfected into PC12 cells by lipofectamin method, and intervened by application of curcumin (20 μmol/L) and 5-hydroxydecanoate (5-HD). Oligomer formation in the cultured cells was identified by Western blotting and Dot blotting. Cytotoxicity and apoptosis of the PC12 cells were measured by lactate dehydrogenase (LDH) and JC-1 assays. mitoKATP were identified by Western blotting and whole cell patch clamp. RESULTS: Curcumin has significantly reduced the oligomer formation induced by overexpression or mutation of α-synuclein in the cultured cells. LDH has decreased by 36.3% and 23.5%, and red/green fluorescence ratio of JC-1 was increased respectively by 48.46% and 50.33% after application of curcumin (P<0.05). Protein expression of Kir6.2 has decreased and mitoKATP channel current has significantly increased (P<0.05). CONCLUSION: Curcumin can inhibit α-synuclein gene overexpression or mutation induced α-synuclein oligomers formation. It may block apoptosis induced by wild-type overexpression or mutation of α-synuclein. By stabilizing mitochondrial membrane potential. Opening of mitoKATP channel may have been the initiating protective mechanism of apoptosis induced by wild-type overexpression or mutation of α-synuclein. Curcumin may antagonize above cytotoxicity through further opening the mitoKATP channel. SN - 1003-9406 UR - https://www.unboundmedicine.com/medline/citation/26252085/[Effect_of_curcumin_on_oligomer_formation_and_mitochondrial_ATP_sensitive_potassium_channels_induced_by_overexpression_or_mutation_of_α_synuclein]_ DB - PRIME DP - Unbound Medicine ER -