Tags

Type your tag names separated by a space and hit enter

Validation of ethnomedicinal potential of Tinospora cordifolia for anticancer and immunomodulatory activities and quantification of bioactive molecules by HPTLC.
J Ethnopharmacol. 2015 Dec 04; 175:131-7.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Tinospora cordifolia (Willd.) Miers ex Hook. f. & Thomas. (Menispermaceae) is one of the most widely used plants in various traditional medicinal systems including "Ayurveda". The plant is used for the treatment of jaundice, rheumatism, urinary disorder, skin diseases, diabetes and anemia. The phytoconstituents present in the plant belongs to different class of compounds such as alkaloids, diterpenoids lactones, glycosides, steroids, phenol, aliphatic compounds and polysaccharides.

AIM OF THE STUDY

The aim of present study was the isolation, structure elucidation, quantification and pharmacological evaluation of secondary metabolites from T. cordifolia for anticancer and immunomodulatory activities.

MATERIALS AND METHODS

Different extracts and fractions were prepared from the stem of T. cordifolia. Pure molecules were isolated using normal phase chromatography and characterized on the basis of NMR and mass spectroscopic techniques. The anti-cancer and immunomodulatory activities of different extracts, fractions and isolated compounds were evaluated against four different human cancer cell lines, KB (human oral squamous carcinoma), CHOK-1 (hamster ovary), HT-29 (human colon cancer) and SiHa (human cervical cancer) and murine primary cells respectively. A simple, normal phase HPTLC method was also developed for the quantification of three bioactive compounds i.e N-formylannonain (1), 11-hydroxymustakone (5) and yangambin (8) in the stem of T. cordifolia hosted on fifteen different plants.

RESULTS

Chromatographic purification of different fractions led to the isolation of eight pure molecules i.e N-formylannonain (1), magnoflorine (2), jatrorrhizine (3) palmatine (4), 11-hydroxymustakone (5), cordifolioside A (6), tinocordiside (7) and yangambin (8). All extracts and fractions were active against KB and CHOK-1 cells whereas among the pure molecules palmatine (4) was found to be active against KB and HT-29; tinocordiside (7) against KB and CHOK-1; yangambin (8) against KB cells however N-formylannonain (1) and 11-hydroxymustakone (5), was found active for immunomodulatory activity. HPTLC quantification of three active molecules i.e N-formylannonain (1), 11-hydroxymustakone (5), and yangambin (8) were found in highest quantity in the stem of T. cordifolia hosted on Mangifera indica, however, other two active molecules were not quantified due to their insufficient quantity.

CONCLUSION

Eight compounds have been isolated and characterized belonging to different classes. The pharmacological evaluation of extract, fractions and pure molecules revealed the ethnomedicinal value of T. cordifolia for anticancer and immunomodulatory activities.

Authors+Show Affiliations

Academy of Scientific and Innovative Research, New Delhi, India; Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176 061, India.Regulatory Research Centre, Department of Biotechnology, CSIR-Institute of Himalayan Bioresource, Palampur, Himachal Pradesh, 176 061, India.Academy of Scientific and Innovative Research, New Delhi, India; Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176 061, India.Academy of Scientific and Innovative Research, New Delhi, India; Natural Product Chemistry and Process Development Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176 061, India. Electronic address: bikram_npp@rediffmail.com.Regulatory Research Centre, Department of Biotechnology, CSIR-Institute of Himalayan Bioresource, Palampur, Himachal Pradesh, 176 061, India. Electronic address: yogendra@ihbt.res.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26253577

Citation

Bala, Manju, et al. "Validation of Ethnomedicinal Potential of Tinospora Cordifolia for Anticancer and Immunomodulatory Activities and Quantification of Bioactive Molecules By HPTLC." Journal of Ethnopharmacology, vol. 175, 2015, pp. 131-7.
Bala M, Pratap K, Verma PK, et al. Validation of ethnomedicinal potential of Tinospora cordifolia for anticancer and immunomodulatory activities and quantification of bioactive molecules by HPTLC. J Ethnopharmacol. 2015;175:131-7.
Bala, M., Pratap, K., Verma, P. K., Singh, B., & Padwad, Y. (2015). Validation of ethnomedicinal potential of Tinospora cordifolia for anticancer and immunomodulatory activities and quantification of bioactive molecules by HPTLC. Journal of Ethnopharmacology, 175, 131-7. https://doi.org/10.1016/j.jep.2015.08.001
Bala M, et al. Validation of Ethnomedicinal Potential of Tinospora Cordifolia for Anticancer and Immunomodulatory Activities and Quantification of Bioactive Molecules By HPTLC. J Ethnopharmacol. 2015 Dec 4;175:131-7. PubMed PMID: 26253577.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validation of ethnomedicinal potential of Tinospora cordifolia for anticancer and immunomodulatory activities and quantification of bioactive molecules by HPTLC. AU - Bala,Manju, AU - Pratap,Kunal, AU - Verma,Praveen Kumar, AU - Singh,Bikram, AU - Padwad,Yogendra, Y1 - 2015/08/05/ PY - 2015/03/04/received PY - 2015/06/26/revised PY - 2015/08/01/accepted PY - 2015/8/9/entrez PY - 2015/8/9/pubmed PY - 2016/10/7/medline KW - Anticancer KW - HPTLC KW - Immunomodulatory KW - Tinospora cordifolia SP - 131 EP - 7 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 175 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Tinospora cordifolia (Willd.) Miers ex Hook. f. & Thomas. (Menispermaceae) is one of the most widely used plants in various traditional medicinal systems including "Ayurveda". The plant is used for the treatment of jaundice, rheumatism, urinary disorder, skin diseases, diabetes and anemia. The phytoconstituents present in the plant belongs to different class of compounds such as alkaloids, diterpenoids lactones, glycosides, steroids, phenol, aliphatic compounds and polysaccharides. AIM OF THE STUDY: The aim of present study was the isolation, structure elucidation, quantification and pharmacological evaluation of secondary metabolites from T. cordifolia for anticancer and immunomodulatory activities. MATERIALS AND METHODS: Different extracts and fractions were prepared from the stem of T. cordifolia. Pure molecules were isolated using normal phase chromatography and characterized on the basis of NMR and mass spectroscopic techniques. The anti-cancer and immunomodulatory activities of different extracts, fractions and isolated compounds were evaluated against four different human cancer cell lines, KB (human oral squamous carcinoma), CHOK-1 (hamster ovary), HT-29 (human colon cancer) and SiHa (human cervical cancer) and murine primary cells respectively. A simple, normal phase HPTLC method was also developed for the quantification of three bioactive compounds i.e N-formylannonain (1), 11-hydroxymustakone (5) and yangambin (8) in the stem of T. cordifolia hosted on fifteen different plants. RESULTS: Chromatographic purification of different fractions led to the isolation of eight pure molecules i.e N-formylannonain (1), magnoflorine (2), jatrorrhizine (3) palmatine (4), 11-hydroxymustakone (5), cordifolioside A (6), tinocordiside (7) and yangambin (8). All extracts and fractions were active against KB and CHOK-1 cells whereas among the pure molecules palmatine (4) was found to be active against KB and HT-29; tinocordiside (7) against KB and CHOK-1; yangambin (8) against KB cells however N-formylannonain (1) and 11-hydroxymustakone (5), was found active for immunomodulatory activity. HPTLC quantification of three active molecules i.e N-formylannonain (1), 11-hydroxymustakone (5), and yangambin (8) were found in highest quantity in the stem of T. cordifolia hosted on Mangifera indica, however, other two active molecules were not quantified due to their insufficient quantity. CONCLUSION: Eight compounds have been isolated and characterized belonging to different classes. The pharmacological evaluation of extract, fractions and pure molecules revealed the ethnomedicinal value of T. cordifolia for anticancer and immunomodulatory activities. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26253577/Validation_of_ethnomedicinal_potential_of_Tinospora_cordifolia_for_anticancer_and_immunomodulatory_activities_and_quantification_of_bioactive_molecules_by_HPTLC_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)30068-4 DB - PRIME DP - Unbound Medicine ER -