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[PECULIARITIES OF GLUCOSE AND GLYCEROL METABOLISM IN Nocardia vaccinii IMB B-7405].
Ukr Biochem J. 2015 Mar-Apr; 87(2):66-75.UB

Abstract

It has been established that in cells of Nocardia vaccinii IMB B-7405 (surfactant producer) glucose catabolism is performed through pentose phosphate cycle as well as through gluconate (activity of NAD+-dependent glucose-6-phosphate dehydrogenase and FAD+-dependent glucose dehydrogenase 835 ± 41 and 698 ± 35 nmol.min-1.mg-1 of protein respectively). 6-Phosphogluconate formed in the gluconokinase reaction is involved in the pentose phosphate cycle (activity of constitutive NADP+-dependent 6-phosphogluconate dehydrogenase 357 ± 17 nmol.min-1.mg-1 of protein). Glycerol catabolism to dihydroxyacetonephosphate (the intermediate of glycolysis) may be performed in two ways: through glycerol-3-phosphate (glycerol kinase activity 244 ± 12 nmol.min-1.mg-1 of protein) and through dihydroxyacetone. Replenishment of the C4-dicarboxylic acids pool in N. vaccinii IMV B-7405 grown on glucose and glycerol occurs in the phosphoenolpyruvate(PEP)carboxylase reaction (714-803 nmol.min-1.mg-1 of protein). 2-Oxoglutarate was involved in tricarboxylic acid cycle by alternate pathway with the participation of 2-oxoglutarate synthase. The observed activity of both key enzymes of gluconeogenesis (PEP-carboxykinase and PEP-synthase), trehalose phosphate synthase and NADP+-dependent glutamate dehydrogenase confirmed the ability of IMV B-7405 strain to the synthesis of surface active glycoand aminolipids, respectively.

Authors

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Pub Type(s)

Journal Article

Language

ukr

PubMed ID

26255340

Citation

Pirog, T P., et al. "[PECULIARITIES of GLUCOSE and GLYCEROL METABOLISM in Nocardia Vaccinii IMB B-7405]." Ukrainian Biochemical Journal, vol. 87, no. 2, 2015, pp. 66-75.
Pirog TP, Shevchuk TA, Beregova KA, et al. [PECULIARITIES OF GLUCOSE AND GLYCEROL METABOLISM IN Nocardia vaccinii IMB B-7405]. Ukr Biochem J. 2015;87(2):66-75.
Pirog, T. P., Shevchuk, T. A., Beregova, K. A., & Kudrya, N. V. (2015). [PECULIARITIES OF GLUCOSE AND GLYCEROL METABOLISM IN Nocardia vaccinii IMB B-7405]. Ukrainian Biochemical Journal, 87(2), 66-75.
Pirog TP, et al. [PECULIARITIES of GLUCOSE and GLYCEROL METABOLISM in Nocardia Vaccinii IMB B-7405]. Ukr Biochem J. 2015 Mar-Apr;87(2):66-75. PubMed PMID: 26255340.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [PECULIARITIES OF GLUCOSE AND GLYCEROL METABOLISM IN Nocardia vaccinii IMB B-7405]. AU - Pirog,T P, AU - Shevchuk,T A, AU - Beregova,K A, AU - Kudrya,N V, PY - 2015/8/11/entrez PY - 2015/8/11/pubmed PY - 2015/8/26/medline SP - 66 EP - 75 JF - Ukrainian biochemical journal JO - Ukr Biochem J VL - 87 IS - 2 N2 - It has been established that in cells of Nocardia vaccinii IMB B-7405 (surfactant producer) glucose catabolism is performed through pentose phosphate cycle as well as through gluconate (activity of NAD+-dependent glucose-6-phosphate dehydrogenase and FAD+-dependent glucose dehydrogenase 835 ± 41 and 698 ± 35 nmol.min-1.mg-1 of protein respectively). 6-Phosphogluconate formed in the gluconokinase reaction is involved in the pentose phosphate cycle (activity of constitutive NADP+-dependent 6-phosphogluconate dehydrogenase 357 ± 17 nmol.min-1.mg-1 of protein). Glycerol catabolism to dihydroxyacetonephosphate (the intermediate of glycolysis) may be performed in two ways: through glycerol-3-phosphate (glycerol kinase activity 244 ± 12 nmol.min-1.mg-1 of protein) and through dihydroxyacetone. Replenishment of the C4-dicarboxylic acids pool in N. vaccinii IMV B-7405 grown on glucose and glycerol occurs in the phosphoenolpyruvate(PEP)carboxylase reaction (714-803 nmol.min-1.mg-1 of protein). 2-Oxoglutarate was involved in tricarboxylic acid cycle by alternate pathway with the participation of 2-oxoglutarate synthase. The observed activity of both key enzymes of gluconeogenesis (PEP-carboxykinase and PEP-synthase), trehalose phosphate synthase and NADP+-dependent glutamate dehydrogenase confirmed the ability of IMV B-7405 strain to the synthesis of surface active glycoand aminolipids, respectively. SN - 2409-4943 UR - https://www.unboundmedicine.com/medline/citation/26255340/[PECULIARITIES_OF_GLUCOSE_AND_GLYCEROL_METABOLISM_IN_Nocardia_vaccinii_IMB_B_7405]_ DB - PRIME DP - Unbound Medicine ER -