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Prevalence of ColV Plasmid-Linked Genes and In Vivo Pathogenicity of Avian Strains of Escherichia coli.
Foodborne Pathog Dis. 2015 Aug; 12(8):679-85.FP

Abstract

Avian pathogenic Escherichia coli (APEC) causes extraintestinal infections in birds, leading to an increase in the cost of poultry production. The ColV plasmid-linked genes iroN, ompT, hlyF, iss, and iutA have previously been suggested to be predictors of the virulence of APEC. In this research, we analyzed the frequencies of these genes in a Brazilian collection of E. coli isolated from birds with colibacillosis (APEC) and from apparently healthy birds (avian fecal [A(fecal)]), as well as from the litter of poultry houses of apparently healthy flocks (avian litter [A(litter)]). All the isolates that harbored ompT also harbored hlyF, so they were considered as one trait for statistical analysis. The relationship between in vivo virulence in 1-day-old chicks, expressed as a pathogenicity score, and the number of genes in each isolate showed that isolates with less than two of the four genes were rarely pathogenic, while most pathogenic isolates contained two or more genes. Nevertheless, about half of the nonpathogenic isolates also harbored two or more genes, in agreement with previous observations that commensal E. coli isolates from the birds' microbiota can serve as a reservoir of virulence genes. Thus, the pentaplex polymerase chain reaction can be used to indicate that a strain carrying none or only one gene would be nonpathogenic, but it cannot be used to indicate that a strain with two to four genes would be an APEC. Isolates allocated to phylogenetic group B2, which is frequently associated with extraintestinal infections, had the highest pathogenicity scores, while isolates allocated to group B1 had the lowest.

Authors+Show Affiliations

1 Departamento de Biofísica, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil . 2 Instituto de Pesquisas Veterinárias Desidério Finamor , Eldorado do Sul, Brazil .1 Departamento de Biofísica, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .2 Instituto de Pesquisas Veterinárias Desidério Finamor , Eldorado do Sul, Brazil .2 Instituto de Pesquisas Veterinárias Desidério Finamor , Eldorado do Sul, Brazil .1 Departamento de Biofísica, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .1 Departamento de Biofísica, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .1 Departamento de Biofísica, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .2 Instituto de Pesquisas Veterinárias Desidério Finamor , Eldorado do Sul, Brazil .2 Instituto de Pesquisas Veterinárias Desidério Finamor , Eldorado do Sul, Brazil .2 Instituto de Pesquisas Veterinárias Desidério Finamor , Eldorado do Sul, Brazil .1 Departamento de Biofísica, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26258262

Citation

de Oliveira, Aline Luísa, et al. "Prevalence of ColV Plasmid-Linked Genes and in Vivo Pathogenicity of Avian Strains of Escherichia Coli." Foodborne Pathogens and Disease, vol. 12, no. 8, 2015, pp. 679-85.
de Oliveira AL, Rocha DA, Finkler F, et al. Prevalence of ColV Plasmid-Linked Genes and In Vivo Pathogenicity of Avian Strains of Escherichia coli. Foodborne Pathog Dis. 2015;12(8):679-85.
de Oliveira, A. L., Rocha, D. A., Finkler, F., de Moraes, L. B., Barbieri, N. L., Pavanelo, D. B., Winkler, C., Grassotti, T. T., de Brito, K. C., de Brito, B. G., & Horn, F. (2015). Prevalence of ColV Plasmid-Linked Genes and In Vivo Pathogenicity of Avian Strains of Escherichia coli. Foodborne Pathogens and Disease, 12(8), 679-85. https://doi.org/10.1089/fpd.2014.1934
de Oliveira AL, et al. Prevalence of ColV Plasmid-Linked Genes and in Vivo Pathogenicity of Avian Strains of Escherichia Coli. Foodborne Pathog Dis. 2015;12(8):679-85. PubMed PMID: 26258262.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of ColV Plasmid-Linked Genes and In Vivo Pathogenicity of Avian Strains of Escherichia coli. AU - de Oliveira,Aline Luísa, AU - Rocha,Débora Assumpção, AU - Finkler,Fabrine, AU - de Moraes,Lucas Brunelli, AU - Barbieri,Nicolle Lima, AU - Pavanelo,Daniel Brisotto, AU - Winkler,Cristina, AU - Grassotti,Tiela Trapp, AU - de Brito,Kelly Cristina Tagliari, AU - de Brito,Benito Guimarães, AU - Horn,Fabiana, PY - 2015/8/11/entrez PY - 2015/8/11/pubmed PY - 2016/5/20/medline SP - 679 EP - 85 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 12 IS - 8 N2 - Avian pathogenic Escherichia coli (APEC) causes extraintestinal infections in birds, leading to an increase in the cost of poultry production. The ColV plasmid-linked genes iroN, ompT, hlyF, iss, and iutA have previously been suggested to be predictors of the virulence of APEC. In this research, we analyzed the frequencies of these genes in a Brazilian collection of E. coli isolated from birds with colibacillosis (APEC) and from apparently healthy birds (avian fecal [A(fecal)]), as well as from the litter of poultry houses of apparently healthy flocks (avian litter [A(litter)]). All the isolates that harbored ompT also harbored hlyF, so they were considered as one trait for statistical analysis. The relationship between in vivo virulence in 1-day-old chicks, expressed as a pathogenicity score, and the number of genes in each isolate showed that isolates with less than two of the four genes were rarely pathogenic, while most pathogenic isolates contained two or more genes. Nevertheless, about half of the nonpathogenic isolates also harbored two or more genes, in agreement with previous observations that commensal E. coli isolates from the birds' microbiota can serve as a reservoir of virulence genes. Thus, the pentaplex polymerase chain reaction can be used to indicate that a strain carrying none or only one gene would be nonpathogenic, but it cannot be used to indicate that a strain with two to four genes would be an APEC. Isolates allocated to phylogenetic group B2, which is frequently associated with extraintestinal infections, had the highest pathogenicity scores, while isolates allocated to group B1 had the lowest. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/26258262/Prevalence_of_ColV_Plasmid_Linked_Genes_and_In_Vivo_Pathogenicity_of_Avian_Strains_of_Escherichia_coli_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2014.1934?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -