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Development of a diagnostic co-dominant marker for stem rust resistance gene Sr47 introgressed from Aegilops speltoides into durum wheat.
Theor Appl Genet. 2015 Dec; 128(12):2367-74.TA

Abstract

KEY MESSAGE

A robust and diagnostic STS marker for stem rust resistance gene Sr47 was developed and validated for marker-assisted selection. Stem rust (caused by Puccinia graminis f. sp. tritici, Pgt) resistance gene Sr47, originally transferred from Aegilops speltoides to durum wheat (Triticum turgidum subsp. durum) line DAS15, confers a high level of resistance to Pgt race TTKSK (Ug99). Recently, the durum Rusty 5D(5B) substitution line was used to reduce the Ae. speltoides segment, and the resulting lines had Sr47 on small Ae. speltoides segments on wheat chromosome arm 2BL. The objective of this study was to develop a robust marker for marker-assisted selection of Sr47. A 200-kb segment of the Brachypodium distachyon genome syntenic with the Sr47 region was used to identify wheat expressed sequence tags (ESTs) homologous to the B. distachyon genes. The wheat EST sequences were then used to develop sequence-tagged site (STS) markers. By analyzing the markers for polymorphism between Rusty and DAS15, we identified a co-dominant STS marker, designated as Xrwgs38, which amplified 175 and 187 bp fragments from wheat chromosome 2B and Ae. speltoides chromosome 2S segments, respectively. The marker co-segregated with the Ae. speltoides segments carrying Sr47 in the families from four BC2F1 plants, including the parent plants for durum lines RWG35 and RWG36 with the pedigree of Rusty/3/Rusty 5D(5B)/DAS15//47-1 5D(5B). Analysis of 62 durum and common wheat cultivars/lines lacking the Sr47 segment indicated that they all possessed the 175-bp allele of Xrwgs38, indicating that it was diagnostic for the small Ae. speltoides segment carrying Sr47. This study demonstrated that Xrwgs38 will facilitate the selection of Sr47 in durum and common wheat breeding.

Authors+Show Affiliations

Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA.Cereal Crops Research Unit, Red River Valley Agricultural Research Center, USDA-ARS, 1605 Albrecht Blvd. North, Fargo, ND, 58102-2765, USA.Cereal Crops Research Unit, Red River Valley Agricultural Research Center, USDA-ARS, 1605 Albrecht Blvd. North, Fargo, ND, 58102-2765, USA.Cereal Crops Research Unit, Red River Valley Agricultural Research Center, USDA-ARS, 1605 Albrecht Blvd. North, Fargo, ND, 58102-2765, USA.Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA.Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA.Cereal Crops Research Unit, Red River Valley Agricultural Research Center, USDA-ARS, 1605 Albrecht Blvd. North, Fargo, ND, 58102-2765, USA. steven.xu@ars.usda.gov.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26260850

Citation

Yu, Guotai, et al. "Development of a Diagnostic Co-dominant Marker for Stem Rust Resistance Gene Sr47 Introgressed From Aegilops Speltoides Into Durum Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 128, no. 12, 2015, pp. 2367-74.
Yu G, Klindworth DL, Friesen TL, et al. Development of a diagnostic co-dominant marker for stem rust resistance gene Sr47 introgressed from Aegilops speltoides into durum wheat. Theor Appl Genet. 2015;128(12):2367-74.
Yu, G., Klindworth, D. L., Friesen, T. L., Faris, J. D., Zhong, S., Rasmussen, J. B., & Xu, S. S. (2015). Development of a diagnostic co-dominant marker for stem rust resistance gene Sr47 introgressed from Aegilops speltoides into durum wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 128(12), 2367-74. https://doi.org/10.1007/s00122-015-2590-1
Yu G, et al. Development of a Diagnostic Co-dominant Marker for Stem Rust Resistance Gene Sr47 Introgressed From Aegilops Speltoides Into Durum Wheat. Theor Appl Genet. 2015;128(12):2367-74. PubMed PMID: 26260850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a diagnostic co-dominant marker for stem rust resistance gene Sr47 introgressed from Aegilops speltoides into durum wheat. AU - Yu,Guotai, AU - Klindworth,Daryl L, AU - Friesen,Timothy L, AU - Faris,Justin D, AU - Zhong,Shaobin, AU - Rasmussen,Jack B, AU - Xu,Steven S, Y1 - 2015/08/11/ PY - 2015/02/16/received PY - 2015/07/23/accepted PY - 2015/8/12/entrez PY - 2015/8/12/pubmed PY - 2016/3/2/medline SP - 2367 EP - 74 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 128 IS - 12 N2 - KEY MESSAGE: A robust and diagnostic STS marker for stem rust resistance gene Sr47 was developed and validated for marker-assisted selection. Stem rust (caused by Puccinia graminis f. sp. tritici, Pgt) resistance gene Sr47, originally transferred from Aegilops speltoides to durum wheat (Triticum turgidum subsp. durum) line DAS15, confers a high level of resistance to Pgt race TTKSK (Ug99). Recently, the durum Rusty 5D(5B) substitution line was used to reduce the Ae. speltoides segment, and the resulting lines had Sr47 on small Ae. speltoides segments on wheat chromosome arm 2BL. The objective of this study was to develop a robust marker for marker-assisted selection of Sr47. A 200-kb segment of the Brachypodium distachyon genome syntenic with the Sr47 region was used to identify wheat expressed sequence tags (ESTs) homologous to the B. distachyon genes. The wheat EST sequences were then used to develop sequence-tagged site (STS) markers. By analyzing the markers for polymorphism between Rusty and DAS15, we identified a co-dominant STS marker, designated as Xrwgs38, which amplified 175 and 187 bp fragments from wheat chromosome 2B and Ae. speltoides chromosome 2S segments, respectively. The marker co-segregated with the Ae. speltoides segments carrying Sr47 in the families from four BC2F1 plants, including the parent plants for durum lines RWG35 and RWG36 with the pedigree of Rusty/3/Rusty 5D(5B)/DAS15//47-1 5D(5B). Analysis of 62 durum and common wheat cultivars/lines lacking the Sr47 segment indicated that they all possessed the 175-bp allele of Xrwgs38, indicating that it was diagnostic for the small Ae. speltoides segment carrying Sr47. This study demonstrated that Xrwgs38 will facilitate the selection of Sr47 in durum and common wheat breeding. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/26260850/Development_of_a_diagnostic_co_dominant_marker_for_stem_rust_resistance_gene_Sr47_introgressed_from_Aegilops_speltoides_into_durum_wheat_ L2 - https://dx.doi.org/10.1007/s00122-015-2590-1 DB - PRIME DP - Unbound Medicine ER -