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Piperine mediates LPS induced inflammatory and catabolic effects in rat intervertebral disc.
Int J Clin Exp Pathol. 2015; 8(6):6203-13.IJ

Abstract

Piperine is an exact of the active phenolic component from Black pepper. It has been reported to have many biological activities including anti-oxidant, anti-inflammatory and anti-tumor effects. Intervertebral disc degeneration (IDD) is a degenerative disease closely relate to inflammation of nucleus pulposus (NP) cells. This study aimed to assess the anti-inflammatory and anti-catabolic effects of piperine in rat intervertebral disc using in vitro and ex vivo analyzes. We demonstrated that piperine could inhibit LPS induced expression and production of inflammatory factors and catabolic proteases in NP cells culture model. It significantly inhibited multiple inflammatory factors and oxidative stress-associated genes (IL-1β, TNF-α, IL-6, iNOS), MMPs (MMP-3, MMP-13), ADAMTS (ADAMTS-4, ADAMTS-5) mRNA expression and NO production in a concentration-dependent manner. Moreover, piperine could reverse the LPS-induced inhibition of gene expression of aggrecan and collagen-II. Histologic and dimethylmethylene blue analysis indicated piperine could also against LPS induced proteoglycan (PG) depletion in a rat intervertebral disc culture model. Western blot results showed that piperine inhibited the LPS-mediated phosphorylation of JNK and activation of NF-κB. Finally, our results demonstrated the ability of piperine to antagonize LPS-mediated inflammation of NP cells and suppression of PG in rat intervertebral disc, suggesting a potential agent for treatment of IDD in future.

Authors+Show Affiliations

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine 639 Zhizaoju Road, Shanghai 200011, China.Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine 639 Zhizaoju Road, Shanghai 200011, China.Zhejiang Chinese Medical University 548 Binwen Road, Hangzhou 310053, Zhejiang, China.Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine 639 Zhizaoju Road, Shanghai 200011, China.Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine 639 Zhizaoju Road, Shanghai 200011, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26261497

Citation

Li, Yan, et al. "Piperine Mediates LPS Induced Inflammatory and Catabolic Effects in Rat Intervertebral Disc." International Journal of Clinical and Experimental Pathology, vol. 8, no. 6, 2015, pp. 6203-13.
Li Y, Li K, Hu Y, et al. Piperine mediates LPS induced inflammatory and catabolic effects in rat intervertebral disc. Int J Clin Exp Pathol. 2015;8(6):6203-13.
Li, Y., Li, K., Hu, Y., Xu, B., & Zhao, J. (2015). Piperine mediates LPS induced inflammatory and catabolic effects in rat intervertebral disc. International Journal of Clinical and Experimental Pathology, 8(6), 6203-13.
Li Y, et al. Piperine Mediates LPS Induced Inflammatory and Catabolic Effects in Rat Intervertebral Disc. Int J Clin Exp Pathol. 2015;8(6):6203-13. PubMed PMID: 26261497.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Piperine mediates LPS induced inflammatory and catabolic effects in rat intervertebral disc. AU - Li,Yan, AU - Li,Kang, AU - Hu,Yiqin, AU - Xu,Bo, AU - Zhao,Jie, Y1 - 2015/05/19/ PY - 2015/04/02/received PY - 2015/05/19/accepted PY - 2015/8/12/entrez PY - 2015/8/12/pubmed PY - 2016/5/25/medline KW - Piperine KW - inflammation KW - intervertebral disc degeneration KW - nucleus pulposus cells SP - 6203 EP - 13 JF - International journal of clinical and experimental pathology JO - Int J Clin Exp Pathol VL - 8 IS - 6 N2 - Piperine is an exact of the active phenolic component from Black pepper. It has been reported to have many biological activities including anti-oxidant, anti-inflammatory and anti-tumor effects. Intervertebral disc degeneration (IDD) is a degenerative disease closely relate to inflammation of nucleus pulposus (NP) cells. This study aimed to assess the anti-inflammatory and anti-catabolic effects of piperine in rat intervertebral disc using in vitro and ex vivo analyzes. We demonstrated that piperine could inhibit LPS induced expression and production of inflammatory factors and catabolic proteases in NP cells culture model. It significantly inhibited multiple inflammatory factors and oxidative stress-associated genes (IL-1β, TNF-α, IL-6, iNOS), MMPs (MMP-3, MMP-13), ADAMTS (ADAMTS-4, ADAMTS-5) mRNA expression and NO production in a concentration-dependent manner. Moreover, piperine could reverse the LPS-induced inhibition of gene expression of aggrecan and collagen-II. Histologic and dimethylmethylene blue analysis indicated piperine could also against LPS induced proteoglycan (PG) depletion in a rat intervertebral disc culture model. Western blot results showed that piperine inhibited the LPS-mediated phosphorylation of JNK and activation of NF-κB. Finally, our results demonstrated the ability of piperine to antagonize LPS-mediated inflammation of NP cells and suppression of PG in rat intervertebral disc, suggesting a potential agent for treatment of IDD in future. SN - 1936-2625 UR - https://www.unboundmedicine.com/medline/citation/26261497/Piperine_mediates_LPS_induced_inflammatory_and_catabolic_effects_in_rat_intervertebral_disc_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26261497/ DB - PRIME DP - Unbound Medicine ER -