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[Application effect of Oncomelania hupensis snail crushers in batch detection].

Abstract

OBJECTIVE

To evaluate the application effect of Oncomelania hupensis snail crusher in batch detection.

METHODS

The O. hupensis snail crushers and triangular flasks for cercariae shedding were made. The uninfected snails were divided into 4 groups with the snail number of 50, 100, 200, 300, respectively, and then they were put in 40 triangular flasks, 10 flasks each group. Three flasks in each group were randomly selected, and 12 schistosome infected O. hupensis snails were put into the flasks (1 snail per flask). The 12 flasks with infected O. hupensis snails were put back to each group and were numbered uniformly. The O. hupensis snails in each group were crushed by the O. hupensis snail crushers, and the crushing rate of snails, and average crushing time and duration were recorded. When all the O. hupensis snails were crushed, water was added into the flasks, and water films from each flask were taken and observed under microscopes, the number of cercariae was recorded and the detection rates of each group were calculated. Meanwhile, the O. hupensis snails in the field were collected and detected by the crushing method and crushing and shedding method with O. hupensis snail crushers, and the coincidence rate of the two methods was calculated.

RESULTS

The crushing rate of snails in all the 4 groups were 100% , the average crushing times of groups with 50, 100, 200, 300 snails were 15.70, 23.20, 32.20, 39.20, respectively, and the average duration was 1.01, 1.70, 2.00, 3.00 min, respectively. Both the crushing time and duration were positively correlated with the number of snails detected (r = 0.68, 0.73, both P <0.01). The detection rates of cercariae in all the 4 groups were 100%. In the field application, 109 snails were detected by the crushing method, 620 ones were detected by the crushing and shedding method with O. hupensis snail crushers, and no infected snails were found, the coincidence rate of the two methods were 100%.

CONCLUSION

The O. hupensis snail crusher with the advances of easy operation and labor-saving is valuable of field promotion.

Authors

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Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26263787

Citation

He, Liang-cai, et al. "[Application Effect of Oncomelania Hupensis Snail Crushers in Batch Detection]." Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi = Chinese Journal of Schistosomiasis Control, vol. 27, no. 2, 2015, pp. 195-6, 205.
He LC, Wang JS, Li HZ, et al. [Application effect of Oncomelania hupensis snail crushers in batch detection]. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2015;27(2):195-6, 205.
He, L. C., Wang, J. S., Li, H. Z., Yuan, M. Z., & Tian, K. Q. (2015). [Application effect of Oncomelania hupensis snail crushers in batch detection]. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi = Chinese Journal of Schistosomiasis Control, 27(2), pp. 195-6, 205.
He LC, et al. [Application Effect of Oncomelania Hupensis Snail Crushers in Batch Detection]. Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2015;27(2):195-6, 205. PubMed PMID: 26263787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Application effect of Oncomelania hupensis snail crushers in batch detection]. AU - He,Liang-cai, AU - Wang,Jia-song, AU - Li,Hua-zhong, AU - Yuan,Mei-zhi, AU - Tian,Ke-qing, PY - 2015/8/13/entrez PY - 2015/8/13/pubmed PY - 2015/9/9/medline SP - 195-6, 205 JF - Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control JO - Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi VL - 27 IS - 2 N2 - OBJECTIVE: To evaluate the application effect of Oncomelania hupensis snail crusher in batch detection. METHODS: The O. hupensis snail crushers and triangular flasks for cercariae shedding were made. The uninfected snails were divided into 4 groups with the snail number of 50, 100, 200, 300, respectively, and then they were put in 40 triangular flasks, 10 flasks each group. Three flasks in each group were randomly selected, and 12 schistosome infected O. hupensis snails were put into the flasks (1 snail per flask). The 12 flasks with infected O. hupensis snails were put back to each group and were numbered uniformly. The O. hupensis snails in each group were crushed by the O. hupensis snail crushers, and the crushing rate of snails, and average crushing time and duration were recorded. When all the O. hupensis snails were crushed, water was added into the flasks, and water films from each flask were taken and observed under microscopes, the number of cercariae was recorded and the detection rates of each group were calculated. Meanwhile, the O. hupensis snails in the field were collected and detected by the crushing method and crushing and shedding method with O. hupensis snail crushers, and the coincidence rate of the two methods was calculated. RESULTS: The crushing rate of snails in all the 4 groups were 100% , the average crushing times of groups with 50, 100, 200, 300 snails were 15.70, 23.20, 32.20, 39.20, respectively, and the average duration was 1.01, 1.70, 2.00, 3.00 min, respectively. Both the crushing time and duration were positively correlated with the number of snails detected (r = 0.68, 0.73, both P <0.01). The detection rates of cercariae in all the 4 groups were 100%. In the field application, 109 snails were detected by the crushing method, 620 ones were detected by the crushing and shedding method with O. hupensis snail crushers, and no infected snails were found, the coincidence rate of the two methods were 100%. CONCLUSION: The O. hupensis snail crusher with the advances of easy operation and labor-saving is valuable of field promotion. SN - 1005-6661 UR - https://www.unboundmedicine.com/medline/citation/26263787/[Application_effect_of_Oncomelania_hupensis_snail_crushers_in_batch_detection]_ DB - PRIME DP - Unbound Medicine ER -