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Long-term engraftment of myogenic progenitors from adipose-derived stem cells and muscle regeneration in dystrophic mice.
Hum Mol Genet. 2015 Nov 01; 24(21):6029-40.HM

Abstract

Stem cell therapy is a promising approach for treating Duchenne muscular dystrophy (DMD); however, its application is hindered by poor cell engraftment. There have been no reports to date describing the efficient generation of myogenic progenitors from adipose-derived stem cells (ADSCs) that can contribute to muscle regeneration. In this study, we examined the in vivo myogenic potential of progenitors differentiated from ADSCs using forskolin, basic fibroblast growth factor, the glycogen synthase kinase 3β inhibitor 6-bromoindirubin-3'-oxime as well as the supernatant of ADSC cultures. The results indicate that a proliferative population of myogenic progenitors can be derived from ADSCs that have characteristics similar to muscle satellite cells and are capable of terminal differentiation into multinucleated myotubes. When transplanted into DMD model mdx mice either by intramuscular injection or systemic delivery, progenitors were successfully engrafted in skeletal muscle for up to 12 weeks, and generated new muscle fibers, restored dystrophin expression and contributed to the satellite cell compartment. These findings highlight the potential application of myogenic progenitors derived from ADSCs to the treatment of muscular dystrophy.

Authors+Show Affiliations

Department of Neurology, The First Affiliated Hospital, and.Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-Sen University, 510080, Guangzhou, Guangdong Province, People's Republic of China.Department of Neurology, The First Affiliated Hospital, and.Department of Neurology, The First Affiliated Hospital, and.Department of Neurology, The First Affiliated Hospital, and.Department of Neurology, The First Affiliated Hospital, and.Department of Neurology, The First Affiliated Hospital, and.Department of Neurology, The First Affiliated Hospital, and chengzhang100@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26264578

Citation

Zhang, Yu, et al. "Long-term Engraftment of Myogenic Progenitors From Adipose-derived Stem Cells and Muscle Regeneration in Dystrophic Mice." Human Molecular Genetics, vol. 24, no. 21, 2015, pp. 6029-40.
Zhang Y, Zhu Y, Li Y, et al. Long-term engraftment of myogenic progenitors from adipose-derived stem cells and muscle regeneration in dystrophic mice. Hum Mol Genet. 2015;24(21):6029-40.
Zhang, Y., Zhu, Y., Li, Y., Cao, J., Zhang, H., Chen, M., Wang, L., & Zhang, C. (2015). Long-term engraftment of myogenic progenitors from adipose-derived stem cells and muscle regeneration in dystrophic mice. Human Molecular Genetics, 24(21), 6029-40. https://doi.org/10.1093/hmg/ddv316
Zhang Y, et al. Long-term Engraftment of Myogenic Progenitors From Adipose-derived Stem Cells and Muscle Regeneration in Dystrophic Mice. Hum Mol Genet. 2015 Nov 1;24(21):6029-40. PubMed PMID: 26264578.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term engraftment of myogenic progenitors from adipose-derived stem cells and muscle regeneration in dystrophic mice. AU - Zhang,Yu, AU - Zhu,Yuling, AU - Li,Yaqin, AU - Cao,Jiqing, AU - Zhang,Huili, AU - Chen,Menglong, AU - Wang,Liang, AU - Zhang,Cheng, Y1 - 2015/08/11/ PY - 2015/03/20/received PY - 2015/07/31/accepted PY - 2015/8/13/entrez PY - 2015/8/13/pubmed PY - 2016/8/3/medline SP - 6029 EP - 40 JF - Human molecular genetics JO - Hum. Mol. Genet. VL - 24 IS - 21 N2 - Stem cell therapy is a promising approach for treating Duchenne muscular dystrophy (DMD); however, its application is hindered by poor cell engraftment. There have been no reports to date describing the efficient generation of myogenic progenitors from adipose-derived stem cells (ADSCs) that can contribute to muscle regeneration. In this study, we examined the in vivo myogenic potential of progenitors differentiated from ADSCs using forskolin, basic fibroblast growth factor, the glycogen synthase kinase 3β inhibitor 6-bromoindirubin-3'-oxime as well as the supernatant of ADSC cultures. The results indicate that a proliferative population of myogenic progenitors can be derived from ADSCs that have characteristics similar to muscle satellite cells and are capable of terminal differentiation into multinucleated myotubes. When transplanted into DMD model mdx mice either by intramuscular injection or systemic delivery, progenitors were successfully engrafted in skeletal muscle for up to 12 weeks, and generated new muscle fibers, restored dystrophin expression and contributed to the satellite cell compartment. These findings highlight the potential application of myogenic progenitors derived from ADSCs to the treatment of muscular dystrophy. SN - 1460-2083 UR - https://www.unboundmedicine.com/medline/citation/26264578/Long_term_engraftment_of_myogenic_progenitors_from_adipose_derived_stem_cells_and_muscle_regeneration_in_dystrophic_mice_ L2 - https://academic.oup.com/hmg/article-lookup/doi/10.1093/hmg/ddv316 DB - PRIME DP - Unbound Medicine ER -