Tags

Type your tag names separated by a space and hit enter

More than just one: multiplicity of Hirudins and Hirudin-like Factors in the Medicinal Leech, Hirudo medicinalis.
Mol Genet Genomics. 2016 Feb; 291(1):227-40.MG

Abstract

Blood-sucking leeches like the medicinal leech, Hirudo medicinalis, have been used for medical purposes since ancient times. During feeding, medicinal leeches transfer a broad range of bioactive substances into the host's wound to prevent premature hemostasis and blood coagulation. Hirudin is probably the best known of these substances. Despite its long history of investigation, recombinant production and clinical use, there still exist conflicting data regarding the primary structure of hirudin. Entirely unclear is the potential biological significance of three different subtypes and many isoforms of hirudins that have been characterized so far. Furthermore, there is only incomplete information on their cDNA sequences and no information at all on gene structures and DNA sequences are available in the databases. Our efforts to fill these gaps revealed the presence of multiple hirudin-encoding genes in the genome of Hirudo medicinalis. We have strong evidence for the expression of all three subtypes of hirudin within individual leeches and for the expression of additional hirudins or hirudin-like factors that may have different biological functions and may be promising candidates for new drugs.

Authors+Show Affiliations

Animal Physiology and Biochemistry, Zoological Institute and Museum, Ernst Moritz Arndt-University, Greifswald, Germany. christian.mueller@uni-greifswald.de.Animal Physiology and Biochemistry, Zoological Institute and Museum, Ernst Moritz Arndt-University, Greifswald, Germany.Animal Physiology and Biochemistry, Zoological Institute and Museum, Ernst Moritz Arndt-University, Greifswald, Germany.Institute of Clinical Chemistry and Laboratory Medicine, University Medicine, Greifswald, Germany.Animal Physiology and Biochemistry, Zoological Institute and Museum, Ernst Moritz Arndt-University, Greifswald, Germany.Animal Physiology and Biochemistry, Zoological Institute and Museum, Ernst Moritz Arndt-University, Greifswald, Germany.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26267058

Citation

Müller, Christian, et al. "More Than Just One: Multiplicity of Hirudins and Hirudin-like Factors in the Medicinal Leech, Hirudo Medicinalis." Molecular Genetics and Genomics : MGG, vol. 291, no. 1, 2016, pp. 227-40.
Müller C, Mescke K, Liebig S, et al. More than just one: multiplicity of Hirudins and Hirudin-like Factors in the Medicinal Leech, Hirudo medicinalis. Mol Genet Genomics. 2016;291(1):227-40.
Müller, C., Mescke, K., Liebig, S., Mahfoud, H., Lemke, S., & Hildebrandt, J. P. (2016). More than just one: multiplicity of Hirudins and Hirudin-like Factors in the Medicinal Leech, Hirudo medicinalis. Molecular Genetics and Genomics : MGG, 291(1), 227-40. https://doi.org/10.1007/s00438-015-1100-0
Müller C, et al. More Than Just One: Multiplicity of Hirudins and Hirudin-like Factors in the Medicinal Leech, Hirudo Medicinalis. Mol Genet Genomics. 2016;291(1):227-40. PubMed PMID: 26267058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - More than just one: multiplicity of Hirudins and Hirudin-like Factors in the Medicinal Leech, Hirudo medicinalis. AU - Müller,Christian, AU - Mescke,Katharina, AU - Liebig,Stephanie, AU - Mahfoud,Hala, AU - Lemke,Sarah, AU - Hildebrandt,Jan-Peter, Y1 - 2015/08/13/ PY - 2015/05/15/received PY - 2015/07/23/accepted PY - 2015/8/13/entrez PY - 2015/8/13/pubmed PY - 2016/6/1/medline KW - Blood coagulation KW - Gene structure KW - Hirudin KW - Hirudo medicinalis SP - 227 EP - 40 JF - Molecular genetics and genomics : MGG JO - Mol. Genet. Genomics VL - 291 IS - 1 N2 - Blood-sucking leeches like the medicinal leech, Hirudo medicinalis, have been used for medical purposes since ancient times. During feeding, medicinal leeches transfer a broad range of bioactive substances into the host's wound to prevent premature hemostasis and blood coagulation. Hirudin is probably the best known of these substances. Despite its long history of investigation, recombinant production and clinical use, there still exist conflicting data regarding the primary structure of hirudin. Entirely unclear is the potential biological significance of three different subtypes and many isoforms of hirudins that have been characterized so far. Furthermore, there is only incomplete information on their cDNA sequences and no information at all on gene structures and DNA sequences are available in the databases. Our efforts to fill these gaps revealed the presence of multiple hirudin-encoding genes in the genome of Hirudo medicinalis. We have strong evidence for the expression of all three subtypes of hirudin within individual leeches and for the expression of additional hirudins or hirudin-like factors that may have different biological functions and may be promising candidates for new drugs. SN - 1617-4623 UR - https://www.unboundmedicine.com/medline/citation/26267058/More_than_just_one:_multiplicity_of_Hirudins_and_Hirudin_like_Factors_in_the_Medicinal_Leech_Hirudo_medicinalis_ L2 - https://dx.doi.org/10.1007/s00438-015-1100-0 DB - PRIME DP - Unbound Medicine ER -