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Long-chain polyunsaturated fatty acids attenuate the IL-1β-induced proinflammatory response in human fetal intestinal epithelial cells.
Pediatr Res. 2015 Dec; 78(6):626-33.PR

Abstract

BACKGROUND

Evidence suggests that excessive inflammation of the immature intestine may predispose premature infants to necrotizing enterocolitis (NEC). We investigated the anti-inflammatory effects of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and arachidonic acid (ARA) in human fetal and adult intestinal epithelial cells (IEC) in primary culture.

METHODS

Human fetal IEC in culture were derived from a healthy fetal small intestine (H4) or resected small intestine of a neonate with NEC (NEC-IEC). Intestinal cell lines Caco2 and NCM460 in culture were used as models for mature IEC. IEC in culture were pretreated with 100 µmol/l palmitic acid (PAL), DHA, EPA, ARA, or ARA+DHA for 48 h and then stimulated with proinflammatory IL-1β.

RESULTS

DHA significantly attenuated IL-1β induced proinflammatory IL-8 and IL-6 protein and mRNA in fetal H4, NEC-IEC, and mature Caco2, NCM460 IEC, compared to control and PAL treatment. DHA downregulated IL-1R1 (IL-1β receptor) and NFk β1 mRNA expression in fetal and adult IEC. ARA had potent anti-inflammatory effects with lower IL-8 and IL-6 (protein and mRNA) in fetal H4 but not in NEC-IEC or adult IEC.

CONCLUSION

The present study provides evidence that DHA and ARA may have important anti-inflammatory functions for prevention of NEC in premature infants.

Authors+Show Affiliations

Mucosal Immunology and Biology Research Center, Massachusetts General Hospital for Children, Boston, Massachusetts.Division of Nutritional Sciences, Cornell University, Ithaca, New York.Division of Nutritional Sciences, Cornell University, Ithaca, New York.Mucosal Immunology and Biology Research Center, Massachusetts General Hospital for Children, Boston, Massachusetts.Mucosal Immunology and Biology Research Center, Massachusetts General Hospital for Children, Boston, Massachusetts.Mucosal Immunology and Biology Research Center, Massachusetts General Hospital for Children, Boston, Massachusetts.Division of Nutritional Sciences, Cornell University, Ithaca, New York.Liverpool School of Tropical Medicine, Liverpool, UK.Department of Pediatrics, University of British Columbia, Vancouver, British Columbia, Canada.Mucosal Immunology and Biology Research Center, Massachusetts General Hospital for Children, Boston, Massachusetts.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

26270575

Citation

Wijendran, Vasuki, et al. "Long-chain Polyunsaturated Fatty Acids Attenuate the IL-1β-induced Proinflammatory Response in Human Fetal Intestinal Epithelial Cells." Pediatric Research, vol. 78, no. 6, 2015, pp. 626-33.
Wijendran V, Brenna JT, Wang DH, et al. Long-chain polyunsaturated fatty acids attenuate the IL-1β-induced proinflammatory response in human fetal intestinal epithelial cells. Pediatr Res. 2015;78(6):626-33.
Wijendran, V., Brenna, J. T., Wang, D. H., Zhu, W., Meng, D., Ganguli, K., Kothapalli, K. S., Requena, P., Innis, S., & Walker, W. A. (2015). Long-chain polyunsaturated fatty acids attenuate the IL-1β-induced proinflammatory response in human fetal intestinal epithelial cells. Pediatric Research, 78(6), 626-33. https://doi.org/10.1038/pr.2015.154
Wijendran V, et al. Long-chain Polyunsaturated Fatty Acids Attenuate the IL-1β-induced Proinflammatory Response in Human Fetal Intestinal Epithelial Cells. Pediatr Res. 2015;78(6):626-33. PubMed PMID: 26270575.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-chain polyunsaturated fatty acids attenuate the IL-1β-induced proinflammatory response in human fetal intestinal epithelial cells. AU - Wijendran,Vasuki, AU - Brenna,J T, AU - Wang,Dong Hao, AU - Zhu,Weishu, AU - Meng,Di, AU - Ganguli,Kriston, AU - Kothapalli,Kumar S D, AU - Requena,Pilar, AU - Innis,Sheila, AU - Walker,W Allan, Y1 - 2015/08/13/ PY - 2015/03/09/received PY - 2015/05/13/accepted PY - 2015/8/14/entrez PY - 2015/8/14/pubmed PY - 2016/9/28/medline SP - 626 EP - 33 JF - Pediatric research JO - Pediatr Res VL - 78 IS - 6 N2 - BACKGROUND: Evidence suggests that excessive inflammation of the immature intestine may predispose premature infants to necrotizing enterocolitis (NEC). We investigated the anti-inflammatory effects of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and arachidonic acid (ARA) in human fetal and adult intestinal epithelial cells (IEC) in primary culture. METHODS: Human fetal IEC in culture were derived from a healthy fetal small intestine (H4) or resected small intestine of a neonate with NEC (NEC-IEC). Intestinal cell lines Caco2 and NCM460 in culture were used as models for mature IEC. IEC in culture were pretreated with 100 µmol/l palmitic acid (PAL), DHA, EPA, ARA, or ARA+DHA for 48 h and then stimulated with proinflammatory IL-1β. RESULTS: DHA significantly attenuated IL-1β induced proinflammatory IL-8 and IL-6 protein and mRNA in fetal H4, NEC-IEC, and mature Caco2, NCM460 IEC, compared to control and PAL treatment. DHA downregulated IL-1R1 (IL-1β receptor) and NFk β1 mRNA expression in fetal and adult IEC. ARA had potent anti-inflammatory effects with lower IL-8 and IL-6 (protein and mRNA) in fetal H4 but not in NEC-IEC or adult IEC. CONCLUSION: The present study provides evidence that DHA and ARA may have important anti-inflammatory functions for prevention of NEC in premature infants. SN - 1530-0447 UR - https://www.unboundmedicine.com/medline/citation/26270575/Long_chain_polyunsaturated_fatty_acids_attenuate_the_IL_1β_induced_proinflammatory_response_in_human_fetal_intestinal_epithelial_cells_ L2 - https://doi.org/10.1038/pr.2015.154 DB - PRIME DP - Unbound Medicine ER -