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Exposure to welding fumes and lower airway infection with Streptococcus pneumoniae.
J Allergy Clin Immunol. 2016 Feb; 137(2):527-534.e7.JA

Abstract

BACKGROUND

Welders are at increased risk of pneumococcal pneumonia. The mechanism for this association is not known. The capacity of pneumococci to adhere to and infect lower airway cells is mediated by host-expressed platelet-activating factor receptor (PAFR).

OBJECTIVE

We sought to assess the effect of mild steel welding fumes (MS-WF) on PAFR-dependent pneumococcal adhesion and infection to human airway cells in vitro and on pneumococcal airway infection in a mouse model.

METHODS

The oxidative potential of MS-WF was assessed by their capacity to reduce antioxidants in vitro. Pneumococcal adhesion and infection of A549, BEAS-2B, and primary human bronchial airway cells were assessed by means of quantitative bacterial culture and expressed as colony-forming units (CFU). After intranasal instillation of MS-WF, mice were infected with Streptococcus pneumoniae, and bronchoalveolar lavage fluid (BALF) and lung CFU values were determined. PAFR protein levels were assessed by using immunofluorescence and immunohistochemistry, and PAFR mRNA expression was assessed by using quantitative PCR. PAFR was blocked by CV-3988, and oxidative stress was attenuated by N-acetylcysteine.

RESULTS

MS-WF exhibited high oxidative potential. In A549 and BEAS-2B cells MS-WF increased pneumococcal adhesion and infection and PAFR protein expression. Both CV-3988 and N-acetylcysteine reduced MS-WF-stimulated pneumococcal adhesion and infection of airway cells. MS-WF increased mouse lung PAFR mRNA expression and increased BALF and lung pneumococcal CFU values. In MS-WF-exposed mice CV-3988 reduced BALF CFU values.

CONCLUSIONS

Hypersusceptibility of welders to pneumococcal pneumonia is in part mediated by the capacity of welding fumes to increase PAFR-dependent pneumococcal adhesion and infection of lower airway cells.

Links

PMC Free PDF

Authors+Show Affiliations

Suri R
Blizard Institute, Queen Mary University of London, London, United Kingdom.
Periselneris J
Centre for Inflammation and Tissue Repair, Department of Medicine, Royal Free and University College Medical School, Rayne Institute, London, United Kingdom.
Lanone S
Inserm U955 Équipe 4, Faculté de Médecine, Créteil, France.
Zeidler-Erdely PC
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV.
Melton G
Welding Institute, Cambridge, United Kingdom.
Palmer KT
MRC Lifecourse Epidemiology Unit, University of Southampton, Southampton, United Kingdom.
Andujar P
Inserm U955 Équipe 4, Faculté de Médecine, Créteil, France.
Antonini JM
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV.
Cohignac V
Inserm U955 Équipe 4, Faculté de Médecine, Créteil, France.
Erdely A
Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV.
Jose RJ
Centre for Inflammation and Tissue Repair, Department of Medicine, Royal Free and University College Medical School, Rayne Institute, London, United Kingdom.
Mudway I
MRC-PHE Centre for Environment and Health, Analytical & Environmental Sciences Division, Faculty of Life Sciences & Medicine, King's College London, London, United Kingdom.
Brown J
Centre for Inflammation and Tissue Repair, Department of Medicine, Royal Free and University College Medical School, Rayne Institute, London, United Kingdom.
Grigg J
Blizard Institute, Queen Mary University of London, London, United Kingdom. Electronic address: j.grigg@qmul.ac.uk.

MeSH

AnimalsBacterial AdhesionBacterial LoadBronchoalveolar Lavage FluidCell LineDisease Models, AnimalDisease SusceptibilityFemaleGene ExpressionHeavy Metal PoisoningHumansMiceOccupational ExposureOxidative StressPlatelet Membrane GlycoproteinsPneumonia, PneumococcalPoisoningRNA, MessengerReceptors, G-Protein-CoupledRespiratory MucosaStreptococcus pneumoniaeWelding

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26277596

Citation

Suri, Reetika, et al. "Exposure to Welding Fumes and Lower Airway Infection With Streptococcus Pneumoniae." The Journal of Allergy and Clinical Immunology, vol. 137, no. 2, 2016, pp. 527-534.e7.
Suri R, Periselneris J, Lanone S, et al. Exposure to welding fumes and lower airway infection with Streptococcus pneumoniae. J Allergy Clin Immunol. 2016;137(2):527-534.e7.
Suri, R., Periselneris, J., Lanone, S., Zeidler-Erdely, P. C., Melton, G., Palmer, K. T., Andujar, P., Antonini, J. M., Cohignac, V., Erdely, A., Jose, R. J., Mudway, I., Brown, J., & Grigg, J. (2016). Exposure to welding fumes and lower airway infection with Streptococcus pneumoniae. The Journal of Allergy and Clinical Immunology, 137(2), 527-e7. https://doi.org/10.1016/j.jaci.2015.06.033
Suri R, et al. Exposure to Welding Fumes and Lower Airway Infection With Streptococcus Pneumoniae. J Allergy Clin Immunol. 2016;137(2):527-534.e7. PubMed PMID: 26277596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exposure to welding fumes and lower airway infection with Streptococcus pneumoniae. AU - Suri,Reetika, AU - Periselneris,Jimstan, AU - Lanone,Sophie, AU - Zeidler-Erdely,Patti C, AU - Melton,Geoffrey, AU - Palmer,Keith T, AU - Andujar,Pascal, AU - Antonini,James M, AU - Cohignac,Vanessa, AU - Erdely,Aaron, AU - Jose,Ricardo J, AU - Mudway,Ian, AU - Brown,Jeremy, AU - Grigg,Jonathan, Y1 - 2015/08/12/ PY - 2015/02/11/received PY - 2015/05/29/revised PY - 2015/06/29/accepted PY - 2015/8/17/entrez PY - 2015/8/19/pubmed PY - 2016/7/1/medline KW - Occupational disease KW - Streptococcus pneumoniae KW - bacterial adhesion and infection KW - platelet-activating factor receptor KW - pneumonia KW - welding fumes SP - 527 EP - 534.e7 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 137 IS - 2 N2 - BACKGROUND: Welders are at increased risk of pneumococcal pneumonia. The mechanism for this association is not known. The capacity of pneumococci to adhere to and infect lower airway cells is mediated by host-expressed platelet-activating factor receptor (PAFR). OBJECTIVE: We sought to assess the effect of mild steel welding fumes (MS-WF) on PAFR-dependent pneumococcal adhesion and infection to human airway cells in vitro and on pneumococcal airway infection in a mouse model. METHODS: The oxidative potential of MS-WF was assessed by their capacity to reduce antioxidants in vitro. Pneumococcal adhesion and infection of A549, BEAS-2B, and primary human bronchial airway cells were assessed by means of quantitative bacterial culture and expressed as colony-forming units (CFU). After intranasal instillation of MS-WF, mice were infected with Streptococcus pneumoniae, and bronchoalveolar lavage fluid (BALF) and lung CFU values were determined. PAFR protein levels were assessed by using immunofluorescence and immunohistochemistry, and PAFR mRNA expression was assessed by using quantitative PCR. PAFR was blocked by CV-3988, and oxidative stress was attenuated by N-acetylcysteine. RESULTS: MS-WF exhibited high oxidative potential. In A549 and BEAS-2B cells MS-WF increased pneumococcal adhesion and infection and PAFR protein expression. Both CV-3988 and N-acetylcysteine reduced MS-WF-stimulated pneumococcal adhesion and infection of airway cells. MS-WF increased mouse lung PAFR mRNA expression and increased BALF and lung pneumococcal CFU values. In MS-WF-exposed mice CV-3988 reduced BALF CFU values. CONCLUSIONS: Hypersusceptibility of welders to pneumococcal pneumonia is in part mediated by the capacity of welding fumes to increase PAFR-dependent pneumococcal adhesion and infection of lower airway cells. SN - 1097-6825 UR - https://www.unboundmedicine.com/medline/citation/26277596/Exposure_to_welding_fumes_and_lower_airway_infection_with_Streptococcus_pneumoniae_ DB - PRIME DP - Unbound Medicine ER -