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Kiwifruit fibre level influences the predicted production and absorption of SCFA in the hindgut of growing pigs using a combined in vivo-in vitro digestion methodology.
Br J Nutr. 2016 04; 115(8):1317-24.BJ

Abstract

Combined in vivo (ileal cannulated pig) and in vitro (faecal inoculum-based fermentation) digestion methodologies were used to predict the production and absorption of SCFA in the hindgut of growing pigs. Ileal and faecal samples were collected from animals (n 7) fed diets containing either 25 or 50 g/kg DM of kiwifruit fibre from added kiwifruit for 14 d. Ileal and faecal SCFA concentrations normalised for food DM intake (DMI) and nutrient digestibility were determined. Ileal digesta were collected and fermented for 38 h using a fresh pig faecal inoculum to predict SCFA production. The predicted hindgut SCFA production along with the determined ileal and faecal SCFA were then used to predict SCFA absorption in the hindgut and total tract organic matter digestibility. The determined ileal and faecal SCFA concentrations (e.g. 8·5 and 4·4 mmol/kg DMI, respectively, for acetic acid for the low-fibre diet) represented only 0·2-3·2 % of the predicted hindgut SCFA production (e.g. 270 mmol/kg DMI for acetic acid). Predicted production and absorption of acetic, butyric and propionic acids were the highest for the high-fibre diet (P0·05). In conclusion, determined ileal and faecal SCFA concentrations represent only a small fraction of total SCFA production, and may therefore be misleading in relation to the effect of diets on SCFA production and absorption. Considerable quantities of SCFA are produced and absorbed in the hindgut of the pig by the fermentation of kiwifruit.

Authors+Show Affiliations

Riddet Institute,Massey Institute of Food Science and Technology,Massey University,Palmerston North,New Zealand.Riddet Institute,Massey Institute of Food Science and Technology,Massey University,Palmerston North,New Zealand.Riddet Institute,Massey Institute of Food Science and Technology,Massey University,Palmerston North,New Zealand.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26277926

Citation

Montoya, Carlos A., et al. "Kiwifruit Fibre Level Influences the Predicted Production and Absorption of SCFA in the Hindgut of Growing Pigs Using a Combined in Vivo-in Vitro Digestion Methodology." The British Journal of Nutrition, vol. 115, no. 8, 2016, pp. 1317-24.
Montoya CA, Rutherfurd SM, Moughan PJ. Kiwifruit fibre level influences the predicted production and absorption of SCFA in the hindgut of growing pigs using a combined in vivo-in vitro digestion methodology. Br J Nutr. 2016;115(8):1317-24.
Montoya, C. A., Rutherfurd, S. M., & Moughan, P. J. (2016). Kiwifruit fibre level influences the predicted production and absorption of SCFA in the hindgut of growing pigs using a combined in vivo-in vitro digestion methodology. The British Journal of Nutrition, 115(8), 1317-24. https://doi.org/10.1017/S0007114515002883
Montoya CA, Rutherfurd SM, Moughan PJ. Kiwifruit Fibre Level Influences the Predicted Production and Absorption of SCFA in the Hindgut of Growing Pigs Using a Combined in Vivo-in Vitro Digestion Methodology. Br J Nutr. 2016;115(8):1317-24. PubMed PMID: 26277926.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kiwifruit fibre level influences the predicted production and absorption of SCFA in the hindgut of growing pigs using a combined in vivo-in vitro digestion methodology. AU - Montoya,Carlos A, AU - Rutherfurd,Shane M, AU - Moughan,Paul J, Y1 - 2015/08/17/ PY - 2015/8/18/entrez PY - 2015/8/19/pubmed PY - 2017/5/6/medline KW - Absorption KW - DMI DM intake KW - GIT gastrointestinal tract KW - Growing pigs KW - In vitro fermentation KW - In vivo digestion KW - Kiwifruit dietary fibre KW - OM organic matter KW - SCFA SP - 1317 EP - 24 JF - The British journal of nutrition JO - Br J Nutr VL - 115 IS - 8 N2 - Combined in vivo (ileal cannulated pig) and in vitro (faecal inoculum-based fermentation) digestion methodologies were used to predict the production and absorption of SCFA in the hindgut of growing pigs. Ileal and faecal samples were collected from animals (n 7) fed diets containing either 25 or 50 g/kg DM of kiwifruit fibre from added kiwifruit for 14 d. Ileal and faecal SCFA concentrations normalised for food DM intake (DMI) and nutrient digestibility were determined. Ileal digesta were collected and fermented for 38 h using a fresh pig faecal inoculum to predict SCFA production. The predicted hindgut SCFA production along with the determined ileal and faecal SCFA were then used to predict SCFA absorption in the hindgut and total tract organic matter digestibility. The determined ileal and faecal SCFA concentrations (e.g. 8·5 and 4·4 mmol/kg DMI, respectively, for acetic acid for the low-fibre diet) represented only 0·2-3·2 % of the predicted hindgut SCFA production (e.g. 270 mmol/kg DMI for acetic acid). Predicted production and absorption of acetic, butyric and propionic acids were the highest for the high-fibre diet (P0·05). In conclusion, determined ileal and faecal SCFA concentrations represent only a small fraction of total SCFA production, and may therefore be misleading in relation to the effect of diets on SCFA production and absorption. Considerable quantities of SCFA are produced and absorbed in the hindgut of the pig by the fermentation of kiwifruit. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/26277926/Kiwifruit_fibre_level_influences_the_predicted_production_and_absorption_of_SCFA_in_the_hindgut_of_growing_pigs_using_a_combined_in_vivo_in_vitro_digestion_methodology_ L2 - https://www.cambridge.org/core/product/identifier/S0007114515002883/type/journal_article DB - PRIME DP - Unbound Medicine ER -