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The effects of upaB deletion and the double/triple deletion of upaB, aatA, and aatB genes on pathogenicity of avian pathogenic Escherichia coli.
Appl Microbiol Biotechnol 2015; 99(24):10639-54AM

Abstract

Autotransporters (ATs) are associated with pathogenesis of Avian Pathogenic Escherichia coli (APEC). The molecular characterization of APEC ATs can provide insights about their relevance to APEC pathogenesis. Here, we characterized a conventional autotransporter UpaB in APEC DE205B genome. The upaB existed in 41.9 % of 236 APEC isolates and was predominantly associated with ECOR B2 and D. Our studies showed that UpaB mediates the DE205B adhesion in DF-1 cells, and enhances autoaggregation and biofilm formation of fimbria-negative E. coli AAEC189 (MG1655Δfim) in vitro. Deletion of upaB of DE205B attenuates the virulence in duck model and early colonization in the duck lungs during APEC systemic infection. Furthermore, double and triple deletion of upaB, aatA, and aatB genes cumulatively attenuated DE205B adhesion in DF-1 cells, accompanying with decreased 50 % lethal dose (LD50) in duck model and the early colonization in the duck lungs. However, DE205BΔupaB/ΔaatA/ΔaatB might "compensate" the influence of gene deletion by upregulating the expression of fimbrial adhesin genes yqiL, yadN, and vacuolating autotransporter vat during early colonization of APEC. Finally, we demonstrated that vaccination with recombinant UpaB, AatA, and AatB proteins conferred protection against colisepticemia caused by DE205B infection in duck model.

Authors+Show Affiliations

Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. zgxiangkai@163.com.Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. 2013207013@njau.edu.cn.Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. 850421558@qq.com.Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China. xmao@njau.edu.cn.Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. 2013807115@njau.edu.cn.Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241, China. 1196820085@qq.com.Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. 1091517919@qq.com.Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. lucp@njau.edu.cn.Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. fhj@njau.edu.cn.Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural, University, No.1 Weigang road, Nanjing, Jiangsu Province, 210095, China. daijianjun@njau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26278540

Citation

Zhu-Ge, Xiang-Kai, et al. "The Effects of upaB Deletion and the Double/triple Deletion of upaB, aatA, and aatB Genes On Pathogenicity of Avian Pathogenic Escherichia Coli." Applied Microbiology and Biotechnology, vol. 99, no. 24, 2015, pp. 10639-54.
Zhu-Ge XK, Pan ZH, Tang F, et al. The effects of upaB deletion and the double/triple deletion of upaB, aatA, and aatB genes on pathogenicity of avian pathogenic Escherichia coli. Appl Microbiol Biotechnol. 2015;99(24):10639-54.
Zhu-Ge, X. K., Pan, Z. H., Tang, F., Mao, X., Hu, L., Wang, S. H., ... Dai, J. J. (2015). The effects of upaB deletion and the double/triple deletion of upaB, aatA, and aatB genes on pathogenicity of avian pathogenic Escherichia coli. Applied Microbiology and Biotechnology, 99(24), pp. 10639-54. doi:10.1007/s00253-015-6925-2.
Zhu-Ge XK, et al. The Effects of upaB Deletion and the Double/triple Deletion of upaB, aatA, and aatB Genes On Pathogenicity of Avian Pathogenic Escherichia Coli. Appl Microbiol Biotechnol. 2015;99(24):10639-54. PubMed PMID: 26278540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of upaB deletion and the double/triple deletion of upaB, aatA, and aatB genes on pathogenicity of avian pathogenic Escherichia coli. AU - Zhu-Ge,Xiang-Kai, AU - Pan,Zi-Hao, AU - Tang,Fang, AU - Mao,Xiang, AU - Hu,Lin, AU - Wang,Shao-Hui, AU - Xu,Bin, AU - Lu,Cheng-Ping, AU - Fan,Hong-Jie, AU - Dai,Jian-Jun, Y1 - 2015/08/18/ PY - 2015/05/26/received PY - 2015/08/05/accepted PY - 2015/07/30/revised PY - 2015/8/18/entrez PY - 2015/8/19/pubmed PY - 2016/9/8/medline KW - APEC KW - Autotransporter UPAB KW - Double/triple deletion KW - Pathogenicity KW - Vaccination SP - 10639 EP - 54 JF - Applied microbiology and biotechnology JO - Appl. Microbiol. Biotechnol. VL - 99 IS - 24 N2 - Autotransporters (ATs) are associated with pathogenesis of Avian Pathogenic Escherichia coli (APEC). The molecular characterization of APEC ATs can provide insights about their relevance to APEC pathogenesis. Here, we characterized a conventional autotransporter UpaB in APEC DE205B genome. The upaB existed in 41.9 % of 236 APEC isolates and was predominantly associated with ECOR B2 and D. Our studies showed that UpaB mediates the DE205B adhesion in DF-1 cells, and enhances autoaggregation and biofilm formation of fimbria-negative E. coli AAEC189 (MG1655Δfim) in vitro. Deletion of upaB of DE205B attenuates the virulence in duck model and early colonization in the duck lungs during APEC systemic infection. Furthermore, double and triple deletion of upaB, aatA, and aatB genes cumulatively attenuated DE205B adhesion in DF-1 cells, accompanying with decreased 50 % lethal dose (LD50) in duck model and the early colonization in the duck lungs. However, DE205BΔupaB/ΔaatA/ΔaatB might "compensate" the influence of gene deletion by upregulating the expression of fimbrial adhesin genes yqiL, yadN, and vacuolating autotransporter vat during early colonization of APEC. Finally, we demonstrated that vaccination with recombinant UpaB, AatA, and AatB proteins conferred protection against colisepticemia caused by DE205B infection in duck model. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/26278540/The_effects_of_upaB_deletion_and_the_double/triple_deletion_of_upaB,_aatA,_and_aatB_genes_on_pathogenicity_of_avian_pathogenic_Escherichia_coli L2 - https://dx.doi.org/10.1007/s00253-015-6925-2 DB - PRIME DP - Unbound Medicine ER -