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Effect of cellular products of potential probiotic bacteria on the immune response of Labeo rohita and susceptibility to Aeromonas hydrophila infection.
Fish Shellfish Immunol. 2015 Oct; 46(2):716-22.FS

Abstract

In the present study, the immunological efficacy of cellular components from the potential probiotic bacteria Bacillus subtilis VSG1, Pseudomonas aeruginosa VSG2, and Lactobacillus plantarum VSG3 was evaluated in Labeo rohita fingerlings. Fish were immunized intraperitoneally with 0.1 mL phosphate-buffer solution (PBS) containing 0.1 mg of any of the following cellular components: intercellular products (ICPs) of VSG1 (BS-ICPs), heat-killed whole cell products of VSG2 (PA-HKWCPs), or ICPs of VSG3 (LP-ICPs). Fish injected with 0.1 mL PBS served as the control. Various immunological parameters, including the expression of immune-related genes, were measured 14 and 21 days post-immunization. The fish were challenged with Aeromonas hydrophila and mortality was recorded up to 21 days post-infection. The results revealed that administration of cellular components significantly increased the activity of serum lysozyme and the alternative complement pathway, phagocytosis, and respiratory bursts throughout the experimental period. Total serum protein, albumin, and globulin levels were significantly higher in experimental groups than in the control up to 14 days post-immunization, and decreased thereafter. With respect to immune-related genes, IL-1β, COX-2, iNOS, and IL-10 were highly (P < 0.05) up-regulated in fish immunized with cellular components, compared to the control. The expression of TNF-α and NF-κB was up-regulated in immunized fish up to 14 days post-immunization. Interestingly, fish immunized with LP-ICPs exhibited a significantly higher post-challenge relative percent survival (83.32%), followed by PA-HKWCPs (66.66%), and BS-ICPs (50%). These results indicate that cellular components of probiotic bacteria can influence immune responses, enhance disease protection, and stimulate immune-related gene expression in rohu. Hence, these cellular components may be useful as adjuvants for vaccines in aquaculture.

Authors+Show Affiliations

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, South Korea. Electronic address: giribiotek@gmail.com.School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address: shibsankar.iicb@gmail.com.Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, South Korea. Electronic address: Chicheng0421@126.com.Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, South Korea. Electronic address: hjoong1@nate.com.Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, South Korea. Electronic address: arseidon@naver.com.Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151742, South Korea. Electronic address: parksec@snu.ac.kr.Dept. of Biotechnology, Periyar Maniammai University, Thanjavur 613403, Tamil Nadu, India. Electronic address: drvsukumar@gmail.com.

Pub Type(s)

Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

26282681

Citation

Giri, Sib Sankar, et al. "Effect of Cellular Products of Potential Probiotic Bacteria On the Immune Response of Labeo Rohita and Susceptibility to Aeromonas Hydrophila Infection." Fish & Shellfish Immunology, vol. 46, no. 2, 2015, pp. 716-22.
Giri SS, Sen SS, Chi C, et al. Effect of cellular products of potential probiotic bacteria on the immune response of Labeo rohita and susceptibility to Aeromonas hydrophila infection. Fish Shellfish Immunol. 2015;46(2):716-22.
Giri, S. S., Sen, S. S., Chi, C., Kim, H. J., Yun, S., Park, S. C., & Sukumaran, V. (2015). Effect of cellular products of potential probiotic bacteria on the immune response of Labeo rohita and susceptibility to Aeromonas hydrophila infection. Fish & Shellfish Immunology, 46(2), 716-22. https://doi.org/10.1016/j.fsi.2015.08.012
Giri SS, et al. Effect of Cellular Products of Potential Probiotic Bacteria On the Immune Response of Labeo Rohita and Susceptibility to Aeromonas Hydrophila Infection. Fish Shellfish Immunol. 2015;46(2):716-22. PubMed PMID: 26282681.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of cellular products of potential probiotic bacteria on the immune response of Labeo rohita and susceptibility to Aeromonas hydrophila infection. AU - Giri,Sib Sankar, AU - Sen,Shib Sankar, AU - Chi,Cheng, AU - Kim,Hyoun Joong, AU - Yun,Saekil, AU - Park,Se Chang, AU - Sukumaran,V, Y1 - 2015/08/14/ PY - 2015/06/19/received PY - 2015/08/10/revised PY - 2015/08/12/accepted PY - 2015/8/19/entrez PY - 2015/8/19/pubmed PY - 2016/7/15/medline KW - Aeromonas hydrophila KW - Cellular components KW - Disease protection KW - Gene expression KW - Immune response KW - Labeo rohita KW - Probiotic bacteria SP - 716 EP - 22 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 46 IS - 2 N2 - In the present study, the immunological efficacy of cellular components from the potential probiotic bacteria Bacillus subtilis VSG1, Pseudomonas aeruginosa VSG2, and Lactobacillus plantarum VSG3 was evaluated in Labeo rohita fingerlings. Fish were immunized intraperitoneally with 0.1 mL phosphate-buffer solution (PBS) containing 0.1 mg of any of the following cellular components: intercellular products (ICPs) of VSG1 (BS-ICPs), heat-killed whole cell products of VSG2 (PA-HKWCPs), or ICPs of VSG3 (LP-ICPs). Fish injected with 0.1 mL PBS served as the control. Various immunological parameters, including the expression of immune-related genes, were measured 14 and 21 days post-immunization. The fish were challenged with Aeromonas hydrophila and mortality was recorded up to 21 days post-infection. The results revealed that administration of cellular components significantly increased the activity of serum lysozyme and the alternative complement pathway, phagocytosis, and respiratory bursts throughout the experimental period. Total serum protein, albumin, and globulin levels were significantly higher in experimental groups than in the control up to 14 days post-immunization, and decreased thereafter. With respect to immune-related genes, IL-1β, COX-2, iNOS, and IL-10 were highly (P < 0.05) up-regulated in fish immunized with cellular components, compared to the control. The expression of TNF-α and NF-κB was up-regulated in immunized fish up to 14 days post-immunization. Interestingly, fish immunized with LP-ICPs exhibited a significantly higher post-challenge relative percent survival (83.32%), followed by PA-HKWCPs (66.66%), and BS-ICPs (50%). These results indicate that cellular components of probiotic bacteria can influence immune responses, enhance disease protection, and stimulate immune-related gene expression in rohu. Hence, these cellular components may be useful as adjuvants for vaccines in aquaculture. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/26282681/Effect_of_cellular_products_of_potential_probiotic_bacteria_on_the_immune_response_of_Labeo_rohita_and_susceptibility_to_Aeromonas_hydrophila_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(15)30102-9 DB - PRIME DP - Unbound Medicine ER -