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Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain from Agroindustrial Residues of Ananas comosus.
Planta Med 2015; 81(18):1719-26PM

Abstract

This works reports the purification of bromelain extracted from Ananas comosus industrial residues by ethanol purification, its partial characterization from the crude extract as well as the ethanol purified enzyme, and its application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels. Bromelain was recovered within the 30-70 % ethanol fraction, which achieved a purification factor of 3.12-fold, and yielded more than 90 % of its initial activity. The resulting purified bromelain contained more than 360 U · mg(-1), with a maximum working temperature of 60 °C and pH of 8.0. Poly(N-isopropylacrylamide)-co-acrylamide hydrogels presented a swelling rate of 125 %, which was capable of loading 56 % of bromelain from the solution, and was able to release up to 91 % of the retained bromelain. Ethanol precipitation is suitable for bromelain recovery and application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels based on its processing time and the applied ethanol prices.

Authors+Show Affiliations

Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil.Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil.Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil.No affiliation info availableSchool of Chemical Engineering, Unicamp, Campinas, SP, Brazil.Faculty of Pharmaceutical Sciences, Unicamp, Campinas, SP, Brazil.Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil.School of Pharmaceutical Sciences, USP, São Paulo, SP, Brazil.School of Chemical Engineering, Unicamp, Campinas, SP, Brazil.Genetics and Biochemistry Institute, UFU, Uberlândia, MG, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26287766

Citation

Croisfelt, Fernanda, et al. "Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain From Agroindustrial Residues of Ananas Comosus." Planta Medica, vol. 81, no. 18, 2015, pp. 1719-26.
Croisfelt F, Martins BC, Rescolino R, et al. Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain from Agroindustrial Residues of Ananas comosus. Planta Med. 2015;81(18):1719-26.
Croisfelt, F., Martins, B. C., Rescolino, R., Coelho, D. F., Zanchetta, B., Mazzola, P. G., ... Silveira, E. (2015). Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain from Agroindustrial Residues of Ananas comosus. Planta Medica, 81(18), pp. 1719-26. doi:10.1055/s-0035-1557867.
Croisfelt F, et al. Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain From Agroindustrial Residues of Ananas Comosus. Planta Med. 2015;81(18):1719-26. PubMed PMID: 26287766.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Poly(N-Isopropylacrylamide)-co-Acrylamide Hydrogels for the Controlled Release of Bromelain from Agroindustrial Residues of Ananas comosus. AU - Croisfelt,Fernanda, AU - Martins,Bianca C, AU - Rescolino,Robson, AU - Coelho,Diego F, AU - Zanchetta,Beatriz, AU - Mazzola,Priscila G, AU - Goulart,Luis Ricardo, AU - Pessoa,Adalberto,Jr AU - Tambourgi,Elias B, AU - Silveira,Edgar, Y1 - 2015/08/19/ PY - 2015/8/20/entrez PY - 2015/8/20/pubmed PY - 2016/8/23/medline SP - 1719 EP - 26 JF - Planta medica JO - Planta Med. VL - 81 IS - 18 N2 - This works reports the purification of bromelain extracted from Ananas comosus industrial residues by ethanol purification, its partial characterization from the crude extract as well as the ethanol purified enzyme, and its application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels. Bromelain was recovered within the 30-70 % ethanol fraction, which achieved a purification factor of 3.12-fold, and yielded more than 90 % of its initial activity. The resulting purified bromelain contained more than 360 U · mg(-1), with a maximum working temperature of 60 °C and pH of 8.0. Poly(N-isopropylacrylamide)-co-acrylamide hydrogels presented a swelling rate of 125 %, which was capable of loading 56 % of bromelain from the solution, and was able to release up to 91 % of the retained bromelain. Ethanol precipitation is suitable for bromelain recovery and application onto poly(N-isopropylacrylamide)-co-acrylamide hydrogels based on its processing time and the applied ethanol prices. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/26287766/Poly_N_Isopropylacrylamide__co_Acrylamide_Hydrogels_for_the_Controlled_Release_of_Bromelain_from_Agroindustrial_Residues_of_Ananas_comosus_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0035-1557867 DB - PRIME DP - Unbound Medicine ER -