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Dermatophyte and non dermatophyte fungi in Riyadh City, Saudi Arabia.
Saudi J Biol Sci. 2015 Sep; 22(5):604-9.SJ

Abstract

BACKGROUND

Dermatophytes are a scientific label for a group of three genera (Microsporum, Epidermophyton and Trichophyton) of fungus that causes skin disease in animals and humans. Conventional methods for identification of these fungi are rapid and simple but are not accurate comparing to molecular methods.

OBJECTIVE

This study aimed to isolate human pathogenic dermatophytes which cause dermatophytosis in Riyadh City, Saudi Arabia and to identify these fungi by using conventional and molecular methods.

METHODS

The study was conducted in Medical Complex, Riyadh and King Saud University. Samples of infected skin, hairs and nails were collected from 112 patients. Diagnosis of skin infections, direct microscopic test, isolation and identification of dermatophytes by conventional and molecular methods were carried out.

RESULTS

The results indicated that the tinea capitis infection had the highest prevalence among the patients (22.3%) while Tinea barbae had the lowest. In this study the identified dermatophyte isolates belong to nine species as Trichophyton violaceum, Trichophyton verrucosum, Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton schoenleinii, Trichophyton concentricum, Microsporum canis, Microsporum audouinii and Epidermophyton floccosum which cause skin infections were isolated during this study. Non dermatophyte isolates included 5 isolates from Aspergillus spp. 4 isolates from Acremonium potronii and 15 isolates from Candida spp. M. canis were the most common species (25% of isolated dermatophytes). Out of the 52 dermatophyte isolates identified by conventional methods, there were 45 isolates identified by the molecular method.

CONCLUSIONS

The results concluded that approximately M. canis caused a quarter of dermatophyte cases, tinea capitis infection was prevalent and the molecular method was more accurate than conventional methods.

Authors+Show Affiliations

Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia ; Department of Biotechnology and Food Technology, Thamar University, Yemen.Agriculture and Veterinary Medicine, Department of Veterinary Medicine, Thamar University, Yemen.Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26288566

Citation

Khaled, Jamal M., et al. "Dermatophyte and Non Dermatophyte Fungi in Riyadh City, Saudi Arabia." Saudi Journal of Biological Sciences, vol. 22, no. 5, 2015, pp. 604-9.
Khaled JM, Golah HA, Khalel AS, et al. Dermatophyte and non dermatophyte fungi in Riyadh City, Saudi Arabia. Saudi J Biol Sci. 2015;22(5):604-9.
Khaled, J. M., Golah, H. A., Khalel, A. S., Alharbi, N. S., & Mothana, R. A. (2015). Dermatophyte and non dermatophyte fungi in Riyadh City, Saudi Arabia. Saudi Journal of Biological Sciences, 22(5), 604-9. https://doi.org/10.1016/j.sjbs.2014.12.006
Khaled JM, et al. Dermatophyte and Non Dermatophyte Fungi in Riyadh City, Saudi Arabia. Saudi J Biol Sci. 2015;22(5):604-9. PubMed PMID: 26288566.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dermatophyte and non dermatophyte fungi in Riyadh City, Saudi Arabia. AU - Khaled,Jamal M, AU - Golah,Hammed A, AU - Khalel,Abdulla S, AU - Alharbi,Naiyf S, AU - Mothana,Ramzi A, Y1 - 2015/01/05/ PY - 2014/09/02/received PY - 2014/11/24/revised PY - 2014/12/15/accepted PY - 2015/8/20/entrez PY - 2015/8/20/pubmed PY - 2015/8/20/medline KW - Dermatophytes KW - Epidermophyton KW - Microsporum KW - Tinea KW - Trichophyton SP - 604 EP - 9 JF - Saudi journal of biological sciences JO - Saudi J Biol Sci VL - 22 IS - 5 N2 - BACKGROUND: Dermatophytes are a scientific label for a group of three genera (Microsporum, Epidermophyton and Trichophyton) of fungus that causes skin disease in animals and humans. Conventional methods for identification of these fungi are rapid and simple but are not accurate comparing to molecular methods. OBJECTIVE: This study aimed to isolate human pathogenic dermatophytes which cause dermatophytosis in Riyadh City, Saudi Arabia and to identify these fungi by using conventional and molecular methods. METHODS: The study was conducted in Medical Complex, Riyadh and King Saud University. Samples of infected skin, hairs and nails were collected from 112 patients. Diagnosis of skin infections, direct microscopic test, isolation and identification of dermatophytes by conventional and molecular methods were carried out. RESULTS: The results indicated that the tinea capitis infection had the highest prevalence among the patients (22.3%) while Tinea barbae had the lowest. In this study the identified dermatophyte isolates belong to nine species as Trichophyton violaceum, Trichophyton verrucosum, Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton schoenleinii, Trichophyton concentricum, Microsporum canis, Microsporum audouinii and Epidermophyton floccosum which cause skin infections were isolated during this study. Non dermatophyte isolates included 5 isolates from Aspergillus spp. 4 isolates from Acremonium potronii and 15 isolates from Candida spp. M. canis were the most common species (25% of isolated dermatophytes). Out of the 52 dermatophyte isolates identified by conventional methods, there were 45 isolates identified by the molecular method. CONCLUSIONS: The results concluded that approximately M. canis caused a quarter of dermatophyte cases, tinea capitis infection was prevalent and the molecular method was more accurate than conventional methods. SN - 1319-562X UR - https://www.unboundmedicine.com/medline/citation/26288566/Dermatophyte_and_non_dermatophyte_fungi_in_Riyadh_City_Saudi_Arabia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1319-562X(14)00192-2 DB - PRIME DP - Unbound Medicine ER -
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