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Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion.
J Hematol Oncol 2015; 8:100JH

Abstract

Herein, we describe the establishment and characterization of the first mixed-phenotype acute leukemia cell line (JIH-5). The JIH-5 cell line was established from leukemia cells with B lymphoid/myeloid phenotype from a female mixed-phenotype acute leukemia patient. JIH-5 cells exhibit an immunophenotype comprised of myeloid and B lymphoid antigens. Whole-exome sequencing revealed somatic mutations in nine genes in JIH-5 cells. Transcriptional sequencing of JIH-5 cells identified EP300-ZNF384 fusion transcript, which is a recurrent alteration in B cell acute lymphoblastic leukemia. Our results suggest that the JIH-5 cell line may serve as a tool for the study of mixed-phenotype acute leukemia or EP300-ZNF384.

Authors+Show Affiliations

Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. ping.chengcheng@163.com. Collaborative Innovation Center of Hematology, Soochow University, Suzhou, People's Republic of China. ping.chengcheng@163.com.Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. qiuhuiying@suda.edu.cn.Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. shirley-1214@163.com.Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. daihaiping8@126.com.Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. changgengruan@hotmail.com. Collaborative Innovation Center of Hematology, Soochow University, Suzhou, People's Republic of China. changgengruan@hotmail.com.DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. Stefan.Ehrentraut@dsmz.de.DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. hdr@dsmz.de.DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany. rml@dsmz.de.Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. chensuning@suda.edu.cn. Collaborative Innovation Center of Hematology, Soochow University, Suzhou, People's Republic of China. chensuning@suda.edu.cn.

Pub Type(s)

Letter
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26293203

Citation

Ping, Nana, et al. "Establishment and Genetic Characterization of a Novel Mixed-phenotype Acute Leukemia Cell Line With EP300-ZNF384 Fusion." Journal of Hematology & Oncology, vol. 8, 2015, p. 100.
Ping N, Qiu H, Wang Q, et al. Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion. J Hematol Oncol. 2015;8:100.
Ping, N., Qiu, H., Wang, Q., Dai, H., Ruan, C., Ehrentraut, S., ... Chen, S. (2015). Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion. Journal of Hematology & Oncology, 8, p. 100. doi:10.1186/s13045-015-0197-2.
Ping N, et al. Establishment and Genetic Characterization of a Novel Mixed-phenotype Acute Leukemia Cell Line With EP300-ZNF384 Fusion. J Hematol Oncol. 2015 Aug 21;8:100. PubMed PMID: 26293203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion. AU - Ping,Nana, AU - Qiu,Huiying, AU - Wang,Qian, AU - Dai,Haiping, AU - Ruan,Changgeng, AU - Ehrentraut,Stefan, AU - Drexler,Hans G, AU - MacLeod,Roderick A F, AU - Chen,Suning, Y1 - 2015/08/21/ PY - 2015/07/15/received PY - 2015/08/11/accepted PY - 2015/8/22/entrez PY - 2015/8/22/pubmed PY - 2016/4/29/medline SP - 100 EP - 100 JF - Journal of hematology & oncology JO - J Hematol Oncol VL - 8 N2 - Herein, we describe the establishment and characterization of the first mixed-phenotype acute leukemia cell line (JIH-5). The JIH-5 cell line was established from leukemia cells with B lymphoid/myeloid phenotype from a female mixed-phenotype acute leukemia patient. JIH-5 cells exhibit an immunophenotype comprised of myeloid and B lymphoid antigens. Whole-exome sequencing revealed somatic mutations in nine genes in JIH-5 cells. Transcriptional sequencing of JIH-5 cells identified EP300-ZNF384 fusion transcript, which is a recurrent alteration in B cell acute lymphoblastic leukemia. Our results suggest that the JIH-5 cell line may serve as a tool for the study of mixed-phenotype acute leukemia or EP300-ZNF384. SN - 1756-8722 UR - https://www.unboundmedicine.com/medline/citation/26293203/Establishment_and_genetic_characterization_of_a_novel_mixed_phenotype_acute_leukemia_cell_line_with_EP300_ZNF384_fusion_ L2 - https://jhoonline.biomedcentral.com/articles/10.1186/s13045-015-0197-2 DB - PRIME DP - Unbound Medicine ER -