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Response of biochemical biomarkers in the aquatic crustacean Daphnia magna exposed to silver nanoparticles.
Environ Sci Pollut Res Int. 2015 Dec; 22(24):19990-9.ES

Abstract

The proliferation of silver nanoparticle (AgNP) production and use owing to their antimicrobial properties justifies the need to examine the resulting environmental impacts. The discharge of biocidal nanoparticles to water bodies may pose a threat to aquatic species. This study evaluated the effects of citrate-coated AgNPs on the standardized test organism Daphnia magna Straus clone MBP996 by means of biochemical biomarker response. AgNP toxicity was compared against the toxic effect of Ag(+). The toxicity endpoints were calculated based upon measured Ag concentrations in exposure media. For AgNPs, the NOAEC and LOAEC values at 48 h were 5 and 7 μg Ag/L, respectively, while these values were 0.5 and 1 μg Ag/L, respectively, for Ag(+). The EC50 at 48 h was computed to be 12.4 ± 0.6 and 2.6 ± 0.1 μg Ag/L for AgNPs and Ag(+), respectively, with 95 % confidence intervals of 12.1-12.8 and 2.3-2.8 μg Ag/L, respectively. These results indicate significant less toxicity of AgNP compared to free Ag(+) ions. Five biomarkers were evaluated in Daphnia magna neonates after acute exposure to Ag(+) or AgNPs, including glutathione (GSH) level, reactive oxygen species (ROS) content, and catalase (CAT), acetylcholinesterase (AChE), and superoxide dismutase (SOD) activity. AgNPs induced toxicity and oxidative stress responses in D. magna neonates at tenfold higher concentrations than Ag. Biochemical methods revealed a clear increase in AChE activity, decreased ROS level, increased GSH level and CAT activity, but no significant changes in SOD activity. As Ag(+) may dissolve from AgNPs, these two types of Ag could act synergistically and produce a greater toxic response. The observed remarkably high toxicity of AgNPs (in the parts-per-billion range) to crustaceans indicates that these organisms are a vulnerable link in the aquatic food chain with regard to contamination by nanosilver. Graphical Abstract ᅟ.

Authors+Show Affiliations

Andrija Štampar Teaching Institute of Public Health, Mirogojska 16, 10 000, Zagreb, Croatia.Andrija Štampar Teaching Institute of Public Health, Mirogojska 16, 10 000, Zagreb, Croatia.Ruđer Bošković Institute, Bijenička cesta 54, 10 000, Zagreb, Croatia.Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10 000, Zagreb, Croatia.Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10 000, Zagreb, Croatia.Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10 000, Zagreb, Croatia.Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10 000, Zagreb, Croatia.Andrija Štampar Teaching Institute of Public Health, Mirogojska 16, 10 000, Zagreb, Croatia.Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10 000, Zagreb, Croatia. ivinkovic@imi.hr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26296504

Citation

Ulm, Lea, et al. "Response of Biochemical Biomarkers in the Aquatic Crustacean Daphnia Magna Exposed to Silver Nanoparticles." Environmental Science and Pollution Research International, vol. 22, no. 24, 2015, pp. 19990-9.
Ulm L, Krivohlavek A, Jurašin D, et al. Response of biochemical biomarkers in the aquatic crustacean Daphnia magna exposed to silver nanoparticles. Environ Sci Pollut Res Int. 2015;22(24):19990-9.
Ulm, L., Krivohlavek, A., Jurašin, D., Ljubojević, M., Šinko, G., Crnković, T., Žuntar, I., Šikić, S., & Vinković Vrček, I. (2015). Response of biochemical biomarkers in the aquatic crustacean Daphnia magna exposed to silver nanoparticles. Environmental Science and Pollution Research International, 22(24), 19990-9. https://doi.org/10.1007/s11356-015-5201-4
Ulm L, et al. Response of Biochemical Biomarkers in the Aquatic Crustacean Daphnia Magna Exposed to Silver Nanoparticles. Environ Sci Pollut Res Int. 2015;22(24):19990-9. PubMed PMID: 26296504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Response of biochemical biomarkers in the aquatic crustacean Daphnia magna exposed to silver nanoparticles. AU - Ulm,Lea, AU - Krivohlavek,Adela, AU - Jurašin,Darija, AU - Ljubojević,Marija, AU - Šinko,Goran, AU - Crnković,Tea, AU - Žuntar,Irena, AU - Šikić,Sandra, AU - Vinković Vrček,Ivana, Y1 - 2015/08/23/ PY - 2015/06/16/received PY - 2015/08/10/accepted PY - 2015/8/23/entrez PY - 2015/8/25/pubmed PY - 2016/7/29/medline KW - Biomarkers KW - Daphnia magna KW - Oxidative stress KW - Silver nanoparticles KW - Toxicity SP - 19990 EP - 9 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 22 IS - 24 N2 - The proliferation of silver nanoparticle (AgNP) production and use owing to their antimicrobial properties justifies the need to examine the resulting environmental impacts. The discharge of biocidal nanoparticles to water bodies may pose a threat to aquatic species. This study evaluated the effects of citrate-coated AgNPs on the standardized test organism Daphnia magna Straus clone MBP996 by means of biochemical biomarker response. AgNP toxicity was compared against the toxic effect of Ag(+). The toxicity endpoints were calculated based upon measured Ag concentrations in exposure media. For AgNPs, the NOAEC and LOAEC values at 48 h were 5 and 7 μg Ag/L, respectively, while these values were 0.5 and 1 μg Ag/L, respectively, for Ag(+). The EC50 at 48 h was computed to be 12.4 ± 0.6 and 2.6 ± 0.1 μg Ag/L for AgNPs and Ag(+), respectively, with 95 % confidence intervals of 12.1-12.8 and 2.3-2.8 μg Ag/L, respectively. These results indicate significant less toxicity of AgNP compared to free Ag(+) ions. Five biomarkers were evaluated in Daphnia magna neonates after acute exposure to Ag(+) or AgNPs, including glutathione (GSH) level, reactive oxygen species (ROS) content, and catalase (CAT), acetylcholinesterase (AChE), and superoxide dismutase (SOD) activity. AgNPs induced toxicity and oxidative stress responses in D. magna neonates at tenfold higher concentrations than Ag. Biochemical methods revealed a clear increase in AChE activity, decreased ROS level, increased GSH level and CAT activity, but no significant changes in SOD activity. As Ag(+) may dissolve from AgNPs, these two types of Ag could act synergistically and produce a greater toxic response. The observed remarkably high toxicity of AgNPs (in the parts-per-billion range) to crustaceans indicates that these organisms are a vulnerable link in the aquatic food chain with regard to contamination by nanosilver. Graphical Abstract ᅟ. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/26296504/Response_of_biochemical_biomarkers_in_the_aquatic_crustacean_Daphnia_magna_exposed_to_silver_nanoparticles_ L2 - https://dx.doi.org/10.1007/s11356-015-5201-4 DB - PRIME DP - Unbound Medicine ER -