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Thymic stromal lymphopoietin-induced interleukin-17A is involved in the development of IgE-mediated atopic dermatitis-like skin lesions in mice.
Immunology 2015; 146(4):568-81I

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with elevated levels of allergen-specific IgE. Although thymic stromal lymphopoietin (TSLP) and interleukin-17A (IL-17A) have been considered as important factors in allergic diseases, their relationships in AD have not been fully defined. Here, we show the contribution of TSLP-induced IL-17A responses to IgE-mediated AD-like skin lesions. BALB/c mice passively sensitized by intraperitoneal injections of ovalbumin (OVA)-specific IgE monoclonal antibody (mAb) were challenged with OVA applied to the skin six times. Treatment with anti-TSLP mAb during the second to sixth challenges inhibited IgE-mediated AD-like skin lesions and IL-17A production in lymph nodes. Furthermore, the increased number of IL-17A-producing CD4(+) and γδ T cells in lymph nodes and neutrophilic inflammation in the skin were reduced by anti-TSLP mAb. These findings prompted us to examine the roles of IL-17A. Treatment with anti-IL-17A mAb suppressed the AD-like skin lesions and neutrophilic inflammation; anti-Gr-1 mAb also inhibited them. Furthermore, treatment with CXCR2 antagonist reduced the AD-like skin lesions and neutrophilic inflammation accompanied by the reduction of IL-17A production; the increased CXCR2 expression in the epidermal cells was suppressed by anti-TSLP mAb. Meanwhile, these treatments, except for anti-Gr-1 mAb, inhibited the increased mast cell accumulation in the skin. Collectively, the mechanism of IgE mediating IL-17A-producing CD4(+) and γδ T cells through TSLP by repeated antigen challenges is involved in AD-like skin lesions associated with skin inflammation, such as neutrophil and mast cell accumulation; TSLP may regulate CXCR2 signalling-induced IL-17A production.

Authors+Show Affiliations

Department of Pharmacology, Kobe Pharmaceutical University, Higashinada, Kobe, Japan.Department of Pharmacology, Kobe Pharmaceutical University, Higashinada, Kobe, Japan. Nutraceutical and Functional Food Research and Development Centre, Prince of Songkla University, Hat-Yai, Songkhla, Thailand.Department of Pharmacology, Kobe Pharmaceutical University, Higashinada, Kobe, Japan.Department of Pharmacology, Kyoto Pharmaceutical University, Yamashina, Kyoto, Japan. Department of Toxicology, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan.Department of Pharmacology, Kobe Pharmaceutical University, Higashinada, Kobe, Japan.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26310839

Citation

Mizutani, Nobuaki, et al. "Thymic Stromal Lymphopoietin-induced interleukin-17A Is Involved in the Development of IgE-mediated Atopic Dermatitis-like Skin Lesions in Mice." Immunology, vol. 146, no. 4, 2015, pp. 568-81.
Mizutani N, Sae-Wong C, Kangsanant S, et al. Thymic stromal lymphopoietin-induced interleukin-17A is involved in the development of IgE-mediated atopic dermatitis-like skin lesions in mice. Immunology. 2015;146(4):568-81.
Mizutani, N., Sae-Wong, C., Kangsanant, S., Nabe, T., & Yoshino, S. (2015). Thymic stromal lymphopoietin-induced interleukin-17A is involved in the development of IgE-mediated atopic dermatitis-like skin lesions in mice. Immunology, 146(4), pp. 568-81. doi:10.1111/imm.12528.
Mizutani N, et al. Thymic Stromal Lymphopoietin-induced interleukin-17A Is Involved in the Development of IgE-mediated Atopic Dermatitis-like Skin Lesions in Mice. Immunology. 2015;146(4):568-81. PubMed PMID: 26310839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thymic stromal lymphopoietin-induced interleukin-17A is involved in the development of IgE-mediated atopic dermatitis-like skin lesions in mice. AU - Mizutani,Nobuaki, AU - Sae-Wong,Chutha, AU - Kangsanant,Sureeporn, AU - Nabe,Takeshi, AU - Yoshino,Shin, Y1 - 2015/09/24/ PY - 2015/05/07/received PY - 2015/08/05/revised PY - 2015/08/14/accepted PY - 2015/8/28/entrez PY - 2015/8/28/pubmed PY - 2016/8/4/medline KW - IgE KW - atopic dermatitis KW - interleukin-17A KW - mast cells KW - neutrophils KW - thymic stromal lymphopoietin SP - 568 EP - 81 JF - Immunology JO - Immunology VL - 146 IS - 4 N2 - Atopic dermatitis (AD) is a chronic inflammatory skin disease associated with elevated levels of allergen-specific IgE. Although thymic stromal lymphopoietin (TSLP) and interleukin-17A (IL-17A) have been considered as important factors in allergic diseases, their relationships in AD have not been fully defined. Here, we show the contribution of TSLP-induced IL-17A responses to IgE-mediated AD-like skin lesions. BALB/c mice passively sensitized by intraperitoneal injections of ovalbumin (OVA)-specific IgE monoclonal antibody (mAb) were challenged with OVA applied to the skin six times. Treatment with anti-TSLP mAb during the second to sixth challenges inhibited IgE-mediated AD-like skin lesions and IL-17A production in lymph nodes. Furthermore, the increased number of IL-17A-producing CD4(+) and γδ T cells in lymph nodes and neutrophilic inflammation in the skin were reduced by anti-TSLP mAb. These findings prompted us to examine the roles of IL-17A. Treatment with anti-IL-17A mAb suppressed the AD-like skin lesions and neutrophilic inflammation; anti-Gr-1 mAb also inhibited them. Furthermore, treatment with CXCR2 antagonist reduced the AD-like skin lesions and neutrophilic inflammation accompanied by the reduction of IL-17A production; the increased CXCR2 expression in the epidermal cells was suppressed by anti-TSLP mAb. Meanwhile, these treatments, except for anti-Gr-1 mAb, inhibited the increased mast cell accumulation in the skin. Collectively, the mechanism of IgE mediating IL-17A-producing CD4(+) and γδ T cells through TSLP by repeated antigen challenges is involved in AD-like skin lesions associated with skin inflammation, such as neutrophil and mast cell accumulation; TSLP may regulate CXCR2 signalling-induced IL-17A production. SN - 1365-2567 UR - https://www.unboundmedicine.com/medline/citation/26310839/Thymic_stromal_lymphopoietin_induced_interleukin_17A_is_involved_in_the_development_of_IgE_mediated_atopic_dermatitis_like_skin_lesions_in_mice_ L2 - https://doi.org/10.1111/imm.12528 DB - PRIME DP - Unbound Medicine ER -