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Chemical and biological characterisation of Machaerium hirtum (Vell.) Stellfeld: absence of cytotoxicity and mutagenicity and possible chemopreventive potential.
Mutagenesis. 2016 Mar; 31(2):147-60.M

Abstract

Machaerium hirtum (Vell.) Stellfeld (M.hirtum) is a plant known as 'jacarandá-bico-de-pato' whose bark is commonly used against diarrhea, cough and cancer. The aim of this study was to phytochemically characterise the hydroethanolic extract of this plant, investigate its antimutagenic activities using the Ames test and evaluate its effects on cell viability, genomic instability, gene expression and cell protection in human hepatocellular carcinoma cells (HepG2). Antimutagenic activity was assessed by simultaneous pre- and post-treatment with direct and indirect mutagens, such as 4-nitro-o-phenylenediamine (NPD), mitomycin C (MMC), benzo[a]pyrene (B[a]P) and aflatoxin B1 (AFB1), using the Ames test, cytokinesis blocking micronucleus and apoptosis assays. Only 3 of the 10 concentrations evaluated in the MTT assay were cytotoxic in HepG2 cells. Micronucleated or apoptotic cells were not observed with any of the tested concentrations, and there were no mutagenic effects in the bacterial system. However, the Nuclear Division Index and flow cytometry data showed a decrease in cell proliferation. The extract showed an inhibitory effect against direct (NPD) and indirect mutagens (B[a]P and AFB1). Furthermore, pre- and post-treated cells showed significant reduction in the number of apoptotic and micronucleated cells. This effect is not likely to be associated with the modulation of antioxidant genes, as shown by the RT-qPCR results. Six known flavonoids were identified in the hydroethanolic extract of Machaerium hirtum leaves, and their structures were elucidated by spectroscopic and spectrophotometric methods. The presence of the antioxidants apigenin and luteolin may explain these protective effects, because these components can inhibit the formation of reactive species and prevent apoptosis and DNA damage. In conclusion, the M.hirtum extract showed chemopreventive potential and was not hazardous at the tested concentrations in the experiments presented here. Moreover, this extract should be investigated further as a chemopreventive agent.

Authors+Show Affiliations

Department of General Biology, Center of Biologic Sciences, State University of Londrina - UEL, Londrina, PR, Brazil.Department of General Biology, Center of Biologic Sciences, State University of Londrina - UEL, Londrina, PR, Brazil.Department of General Biology, Center of Biologic Sciences, State University of Londrina - UEL, Londrina, PR, Brazil.Department of General Biology, Center of Biologic Sciences, State University of Londrina - UEL, Londrina, PR, Brazil, Department of Biological Sciences, Faculty of Pharmaceutical Sciences of Araraquara, São Paulo State University - UNESP, Araraquara, SP, Brazil.Department of General Biology, Center of Biologic Sciences, State University of Londrina - UEL, Londrina, PR, Brazil.Coastal Campus, São Paulo State University - UNESP, São Vicente, SP, Brazil.Coastal Campus, São Paulo State University - UNESP, São Vicente, SP, Brazil.Department of Biological Sciences, Faculty of Pharmaceutical Sciences of Araraquara, São Paulo State University - UNESP, Araraquara, SP, Brazil.Department of Biological Sciences, Faculty of Pharmaceutical Sciences of Araraquara, São Paulo State University - UNESP, Araraquara, SP, Brazil.Department of Biological Sciences, Faculty of Pharmaceutical Sciences of Araraquara, São Paulo State University - UNESP, Araraquara, SP, Brazil.Institute of Biological Research Clemente Estable, Montevideo, Uruguay and.School of Arts, Science and Humanities, São Paulo University-USP, São Paulo, SP, Brazil.Department of General Biology, Center of Biologic Sciences, State University of Londrina - UEL, Londrina, PR, Brazil, ilcecolus@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26314304

Citation

Ribeiro, Diego Luis, et al. "Chemical and Biological Characterisation of Machaerium Hirtum (Vell.) Stellfeld: Absence of Cytotoxicity and Mutagenicity and Possible Chemopreventive Potential." Mutagenesis, vol. 31, no. 2, 2016, pp. 147-60.
Ribeiro DL, Cilião HL, Specian AF, et al. Chemical and biological characterisation of Machaerium hirtum (Vell.) Stellfeld: absence of cytotoxicity and mutagenicity and possible chemopreventive potential. Mutagenesis. 2016;31(2):147-60.
Ribeiro, D. L., Cilião, H. L., Specian, A. F., Serpeloni, J. M., de Souza, M. F., Tangerina, M. M., Vilegas, W., Boldrin, P. K., Resende, F. A., Varanda, E. A., Martínez-López, W., Sannomiya, M., & Cólus, I. M. (2016). Chemical and biological characterisation of Machaerium hirtum (Vell.) Stellfeld: absence of cytotoxicity and mutagenicity and possible chemopreventive potential. Mutagenesis, 31(2), 147-60. https://doi.org/10.1093/mutage/gev066
Ribeiro DL, et al. Chemical and Biological Characterisation of Machaerium Hirtum (Vell.) Stellfeld: Absence of Cytotoxicity and Mutagenicity and Possible Chemopreventive Potential. Mutagenesis. 2016;31(2):147-60. PubMed PMID: 26314304.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical and biological characterisation of Machaerium hirtum (Vell.) Stellfeld: absence of cytotoxicity and mutagenicity and possible chemopreventive potential. AU - Ribeiro,Diego Luis, AU - Cilião,Heloísa Lizotti, AU - Specian,Ana Flávia Leal, AU - Serpeloni,Juliana Mara, AU - de Souza,Marilesia Ferreira, AU - Tangerina,Marcelo Marucci Pereira, AU - Vilegas,Wagner, AU - Boldrin,Paula Karina, AU - Resende,Flávia Aparecida, AU - Varanda,Eliana Aparecida, AU - Martínez-López,Wilner, AU - Sannomiya,Miriam, AU - Cólus,Ilce Mara Syllos, Y1 - 2015/08/27/ PY - 2015/8/29/entrez PY - 2015/9/1/pubmed PY - 2016/11/5/medline SP - 147 EP - 60 JF - Mutagenesis JO - Mutagenesis VL - 31 IS - 2 N2 - Machaerium hirtum (Vell.) Stellfeld (M.hirtum) is a plant known as 'jacarandá-bico-de-pato' whose bark is commonly used against diarrhea, cough and cancer. The aim of this study was to phytochemically characterise the hydroethanolic extract of this plant, investigate its antimutagenic activities using the Ames test and evaluate its effects on cell viability, genomic instability, gene expression and cell protection in human hepatocellular carcinoma cells (HepG2). Antimutagenic activity was assessed by simultaneous pre- and post-treatment with direct and indirect mutagens, such as 4-nitro-o-phenylenediamine (NPD), mitomycin C (MMC), benzo[a]pyrene (B[a]P) and aflatoxin B1 (AFB1), using the Ames test, cytokinesis blocking micronucleus and apoptosis assays. Only 3 of the 10 concentrations evaluated in the MTT assay were cytotoxic in HepG2 cells. Micronucleated or apoptotic cells were not observed with any of the tested concentrations, and there were no mutagenic effects in the bacterial system. However, the Nuclear Division Index and flow cytometry data showed a decrease in cell proliferation. The extract showed an inhibitory effect against direct (NPD) and indirect mutagens (B[a]P and AFB1). Furthermore, pre- and post-treated cells showed significant reduction in the number of apoptotic and micronucleated cells. This effect is not likely to be associated with the modulation of antioxidant genes, as shown by the RT-qPCR results. Six known flavonoids were identified in the hydroethanolic extract of Machaerium hirtum leaves, and their structures were elucidated by spectroscopic and spectrophotometric methods. The presence of the antioxidants apigenin and luteolin may explain these protective effects, because these components can inhibit the formation of reactive species and prevent apoptosis and DNA damage. In conclusion, the M.hirtum extract showed chemopreventive potential and was not hazardous at the tested concentrations in the experiments presented here. Moreover, this extract should be investigated further as a chemopreventive agent. SN - 1464-3804 UR - https://www.unboundmedicine.com/medline/citation/26314304/Chemical_and_biological_characterisation_of_Machaerium_hirtum__Vell___Stellfeld:_absence_of_cytotoxicity_and_mutagenicity_and_possible_chemopreventive_potential_ L2 - https://academic.oup.com/mutage/article-lookup/doi/10.1093/mutage/gev066 DB - PRIME DP - Unbound Medicine ER -