Tags

Type your tag names separated by a space and hit enter

A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis.
Nat Commun. 2015 Aug 28; 6:8041.NC

Abstract

YUCCA (YUC) proteins constitute a family of flavin monooxygenases (FMOs), with an important role in auxin (IAA) biosynthesis. Here we report that Arabidopsis plants overexpressing YUC6 display enhanced IAA-related phenotypes and exhibit improved drought stress tolerance, low rate of water loss and controlled ROS accumulation under drought and oxidative stresses. Co-overexpression of an IAA-conjugating enzyme reduces IAA levels but drought stress tolerance is unaffected, indicating that the stress-related phenotype is not based on IAA overproduction. YUC6 contains a previously unrecognized FAD- and NADPH-dependent thiol-reductase activity (TR) that overlaps with the FMO domain involved in IAA biosynthesis. Mutation of a conserved cysteine residue (Cys-85) preserves FMO but suppresses TR activity and stress tolerance, whereas mutating the FAD- and NADPH-binding sites, that are common to TR and FMO domains, abolishes all outputs. We provide a paradigm for a single protein playing a dual role, regulating plant development and conveying stress defence responses.

Links

PMC Free PDF

Authors+Show Affiliations

Cha JY
Division of Applied Life Science (BK21Plus), PMBBRC &IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Kim WY
Division of Applied Life Science (BK21Plus), PMBBRC &IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Kang SB
Division of Applied Life Science (BK21Plus), PMBBRC &IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Kim JI
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Baek D
Division of Applied Life Science (BK21Plus), PMBBRC &IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Jung IJ
Division of Applied Life Science (BK21Plus), PMBBRC &IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Kim MR
Division of Applied Life Science (BK21Plus), PMBBRC &IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Li N
Division of Applied Life Science (BK21Plus), PMBBRC &IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Kim HJ
Division of Applied Life Science (BK21Plus), PMBBRC &IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Nakajima M
Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi, Saitama 332-0012, Japan.
Asami T
Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi, Saitama 332-0012, Japan. Department of Biochemistry, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia.
Sabir JS
Biotechnology Research Group, Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia.
Park HC
Department of Ecological Adaptation, National Institute of Ecology, Seocheon 325-813, Republic of Korea.
Lee SY
Division of Applied Life Science (BK21Plus), PMBBRC &IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Bohnert HJ
Biotechnology Research Group, Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia. Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Bressan RA
Biotechnology Research Group, Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah 21589, Kingdom of Saudi Arabia. Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907, USA.
Pardo JM
Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Cientificas, Sevilla 41012, Spain.
Yun DJ
Division of Applied Life Science (BK21Plus), PMBBRC &IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.

MeSH

Adaptation, PhysiologicalArabidopsisArabidopsis ProteinsDroughtsGene Expression Regulation, PlantIndoleacetic AcidsMixed Function OxygenasesMutationOxidative StressOxidoreductasesPhenotypeReactive Oxygen SpeciesStress, PhysiologicalSulfhydryl Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26314500

Citation

Cha, Joon-Yung, et al. "A Novel Thiol-reductase Activity of Arabidopsis YUC6 Confers Drought Tolerance Independently of Auxin Biosynthesis." Nature Communications, vol. 6, 2015, p. 8041.
Cha JY, Kim WY, Kang SB, et al. A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis. Nat Commun. 2015;6:8041.
Cha, J. Y., Kim, W. Y., Kang, S. B., Kim, J. I., Baek, D., Jung, I. J., Kim, M. R., Li, N., Kim, H. J., Nakajima, M., Asami, T., Sabir, J. S., Park, H. C., Lee, S. Y., Bohnert, H. J., Bressan, R. A., Pardo, J. M., & Yun, D. J. (2015). A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis. Nature Communications, 6, 8041. https://doi.org/10.1038/ncomms9041
Cha JY, et al. A Novel Thiol-reductase Activity of Arabidopsis YUC6 Confers Drought Tolerance Independently of Auxin Biosynthesis. Nat Commun. 2015 Aug 28;6:8041. PubMed PMID: 26314500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis. AU - Cha,Joon-Yung, AU - Kim,Woe-Yeon, AU - Kang,Sun Bin, AU - Kim,Jeong Im, AU - Baek,Dongwon, AU - Jung,In Jung, AU - Kim,Mi Ri, AU - Li,Ning, AU - Kim,Hyun-Jin, AU - Nakajima,Masatoshi, AU - Asami,Tadao, AU - Sabir,Jamal S M, AU - Park,Hyeong Cheol, AU - Lee,Sang Yeol, AU - Bohnert,Hans J, AU - Bressan,Ray A, AU - Pardo,Jose M, AU - Yun,Dae-Jin, Y1 - 2015/08/28/ PY - 2015/02/27/received PY - 2015/07/11/accepted PY - 2015/8/29/entrez PY - 2015/9/1/pubmed PY - 2016/4/27/medline SP - 8041 EP - 8041 JF - Nature communications JO - Nat Commun VL - 6 N2 - YUCCA (YUC) proteins constitute a family of flavin monooxygenases (FMOs), with an important role in auxin (IAA) biosynthesis. Here we report that Arabidopsis plants overexpressing YUC6 display enhanced IAA-related phenotypes and exhibit improved drought stress tolerance, low rate of water loss and controlled ROS accumulation under drought and oxidative stresses. Co-overexpression of an IAA-conjugating enzyme reduces IAA levels but drought stress tolerance is unaffected, indicating that the stress-related phenotype is not based on IAA overproduction. YUC6 contains a previously unrecognized FAD- and NADPH-dependent thiol-reductase activity (TR) that overlaps with the FMO domain involved in IAA biosynthesis. Mutation of a conserved cysteine residue (Cys-85) preserves FMO but suppresses TR activity and stress tolerance, whereas mutating the FAD- and NADPH-binding sites, that are common to TR and FMO domains, abolishes all outputs. We provide a paradigm for a single protein playing a dual role, regulating plant development and conveying stress defence responses. SN - 2041-1723 UR - https://www.unboundmedicine.com/medline/citation/26314500/A_novel_thiol_reductase_activity_of_Arabidopsis_YUC6_confers_drought_tolerance_independently_of_auxin_biosynthesis_ DB - PRIME DP - Unbound Medicine ER -