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Effects of dietary fiber on cecal short-chain fatty acid and cecal microbiota of broiler and laying-hen chicks.
Poult Sci 2015; 94(10):2351-9PS

Abstract

This experiment was conducted to evaluate the effects of feeding dietary fiber on cecal short-chain fatty acid (SCFA) concentration and cecal microbiota of broiler and laying-hen chicks. The lower fiber diet was based on corn-soybean meal (SBM) and the higher fiber diet was formulated using corn-SBM-dried distillers grains with solubles (DDGS) and wheat bran to contain 60.0 g/kg of both DDGS and wheat bran from 1 to 12 d and 80.0 g/kg of both DDGS and wheat bran from 13 to 21 d. Diets were formulated to meet or exceed NRC nutrient requirements. Broiler and laying-hen chicks were randomly assigned to the high and low fiber diets with 11 replicates of 8 chicks for each of the 4 treatments. One cecum from 3 chicks was collected from each replicate: one cecum underwent SCFA concentration analysis, one underwent bacterial DNA isolation for terminal restriction fragment length polymorphism (TRFLP), and the third cecum was used for metagenomics analyses. There were interactions between bird line and dietary fiber for acetic acid (P = 0.04) and total SCFA (P = 0.04) concentration. There was higher concentration of acetic acid (P = 0.02) and propionic acid (P < 0.01) in broiler chicks compared to laying-hen chicks. TRFLP analysis showed that cecal microbiota varied due to diet (P = 0.02) and chicken line (P = 0.03). Metagenomics analyses identified differences in the relative abundance of Helicobacter pullorum and Megamonas hypermegale and the genera Enterobacteriaceae, Campylobacter, Faecalibacterium, and Bacteroides in different treatment groups. These results provide insights into the effect of dietary fiber on SCFA concentration and modulation of cecal microbiota in broiler and laying-hen chicks.

Authors+Show Affiliations

Department of Animal Science, Iowa State University, Ames, IA 50011, United States.Department of Animal Science, Iowa State University, Ames, IA 50011, United States.Department of Biomedical Sciences, Iowa State University, Ames, IA 50011, United States.Department of Animal Science, Iowa State University, Ames, IA 50011, United States.Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, United States.Department of Animal Science, Iowa State University, Ames, IA 50011, United States mfrothsc@iastate.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26316341

Citation

Walugembe, M, et al. "Effects of Dietary Fiber On Cecal Short-chain Fatty Acid and Cecal Microbiota of Broiler and Laying-hen Chicks." Poultry Science, vol. 94, no. 10, 2015, pp. 2351-9.
Walugembe M, Hsieh JC, Koszewski NJ, et al. Effects of dietary fiber on cecal short-chain fatty acid and cecal microbiota of broiler and laying-hen chicks. Poult Sci. 2015;94(10):2351-9.
Walugembe, M., Hsieh, J. C., Koszewski, N. J., Lamont, S. J., Persia, M. E., & Rothschild, M. F. (2015). Effects of dietary fiber on cecal short-chain fatty acid and cecal microbiota of broiler and laying-hen chicks. Poultry Science, 94(10), pp. 2351-9. doi:10.3382/ps/pev242.
Walugembe M, et al. Effects of Dietary Fiber On Cecal Short-chain Fatty Acid and Cecal Microbiota of Broiler and Laying-hen Chicks. Poult Sci. 2015;94(10):2351-9. PubMed PMID: 26316341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary fiber on cecal short-chain fatty acid and cecal microbiota of broiler and laying-hen chicks. AU - Walugembe,M, AU - Hsieh,J C F, AU - Koszewski,N J, AU - Lamont,S J, AU - Persia,M E, AU - Rothschild,M F, Y1 - 2015/08/27/ PY - 2015/07/05/accepted PY - 2015/05/11/received PY - 2015/8/29/entrez PY - 2015/9/1/pubmed PY - 2015/11/11/medline KW - broiler KW - ceca microbiota KW - layer KW - short-chain fatty acid SP - 2351 EP - 9 JF - Poultry science JO - Poult. Sci. VL - 94 IS - 10 N2 - This experiment was conducted to evaluate the effects of feeding dietary fiber on cecal short-chain fatty acid (SCFA) concentration and cecal microbiota of broiler and laying-hen chicks. The lower fiber diet was based on corn-soybean meal (SBM) and the higher fiber diet was formulated using corn-SBM-dried distillers grains with solubles (DDGS) and wheat bran to contain 60.0 g/kg of both DDGS and wheat bran from 1 to 12 d and 80.0 g/kg of both DDGS and wheat bran from 13 to 21 d. Diets were formulated to meet or exceed NRC nutrient requirements. Broiler and laying-hen chicks were randomly assigned to the high and low fiber diets with 11 replicates of 8 chicks for each of the 4 treatments. One cecum from 3 chicks was collected from each replicate: one cecum underwent SCFA concentration analysis, one underwent bacterial DNA isolation for terminal restriction fragment length polymorphism (TRFLP), and the third cecum was used for metagenomics analyses. There were interactions between bird line and dietary fiber for acetic acid (P = 0.04) and total SCFA (P = 0.04) concentration. There was higher concentration of acetic acid (P = 0.02) and propionic acid (P < 0.01) in broiler chicks compared to laying-hen chicks. TRFLP analysis showed that cecal microbiota varied due to diet (P = 0.02) and chicken line (P = 0.03). Metagenomics analyses identified differences in the relative abundance of Helicobacter pullorum and Megamonas hypermegale and the genera Enterobacteriaceae, Campylobacter, Faecalibacterium, and Bacteroides in different treatment groups. These results provide insights into the effect of dietary fiber on SCFA concentration and modulation of cecal microbiota in broiler and laying-hen chicks. SN - 0032-5791 UR - https://www.unboundmedicine.com/medline/citation/26316341/Effects_of_dietary_fiber_on_cecal_short_chain_fatty_acid_and_cecal_microbiota_of_broiler_and_laying_hen_chicks_ L2 - https://academic.oup.com/ps/article-lookup/doi/10.3382/ps/pev242 DB - PRIME DP - Unbound Medicine ER -