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The Diversity and Geographical Structure of Orientia tsutsugamushi Strains from Scrub Typhus Patients in Laos.
PLoS Negl Trop Dis. 2015; 9(8):e0004024.PN

Abstract

Orientia tsutsugamushi is the causative agent of scrub typhus, a disease transmitted by Leptotrombidium mites which is responsible for a severe and under-reported public health burden throughout Southeast Asia. Here we use multilocus sequence typing (MLST) to characterize 74 clinical isolates from three geographic locations in the Lao PDR (Laos), and compare them with isolates described from Udon Thani, northeast Thailand. The data confirm high levels of diversity and recombination within the natural O. tsutsugamushi population, and a rate of mixed infection of ~8%. We compared the relationships and geographical structuring of the strains and populations using allele based approaches (eBURST), phylogenetic approaches, and by calculating F-statistics (FST). These analyses all point towards low levels of population differentiation between isolates from Vientiane and Udon Thani, cities which straddle the Mekong River which defines the Lao/Thai border, but with a very distinct population in Salavan, southern Laos. These data highlight how land use, as well as the movement of hosts and vectors, may impact on the epidemiology of zoonotic infections.

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Authors+Show Affiliations

Phetsouvanh R
Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos PDR.
Sonthayanon P
Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Pukrittayakamee S
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Paris DH
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Centre for Tropical Medicine & Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom.
Newton PN
Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Laos PDR.
Feil EJ
Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom.
Day NP
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Centre for Tropical Medicine & Global Health, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom.

MeSH

AnimalsGenetic VariationHumansLaosMolecular Sequence DataMultilocus Sequence TypingOrientia tsutsugamushiPhylogenyProspective StudiesScrub TyphusThailand

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26317624

Citation

Phetsouvanh, Rattanaphone, et al. "The Diversity and Geographical Structure of Orientia Tsutsugamushi Strains From Scrub Typhus Patients in Laos." PLoS Neglected Tropical Diseases, vol. 9, no. 8, 2015, pp. e0004024.
Phetsouvanh R, Sonthayanon P, Pukrittayakamee S, et al. The Diversity and Geographical Structure of Orientia tsutsugamushi Strains from Scrub Typhus Patients in Laos. PLoS Negl Trop Dis. 2015;9(8):e0004024.
Phetsouvanh, R., Sonthayanon, P., Pukrittayakamee, S., Paris, D. H., Newton, P. N., Feil, E. J., & Day, N. P. (2015). The Diversity and Geographical Structure of Orientia tsutsugamushi Strains from Scrub Typhus Patients in Laos. PLoS Neglected Tropical Diseases, 9(8), e0004024. https://doi.org/10.1371/journal.pntd.0004024
Phetsouvanh R, et al. The Diversity and Geographical Structure of Orientia Tsutsugamushi Strains From Scrub Typhus Patients in Laos. PLoS Negl Trop Dis. 2015;9(8):e0004024. PubMed PMID: 26317624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Diversity and Geographical Structure of Orientia tsutsugamushi Strains from Scrub Typhus Patients in Laos. AU - Phetsouvanh,Rattanaphone, AU - Sonthayanon,Piengchan, AU - Pukrittayakamee,Sasithon, AU - Paris,Daniel H, AU - Newton,Paul N, AU - Feil,Edward J, AU - Day,Nicholas P J, Y1 - 2015/08/28/ PY - 2015/04/04/received PY - 2015/08/02/accepted PY - 2015/8/29/entrez PY - 2015/9/1/pubmed PY - 2016/4/23/medline SP - e0004024 EP - e0004024 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 9 IS - 8 N2 - Orientia tsutsugamushi is the causative agent of scrub typhus, a disease transmitted by Leptotrombidium mites which is responsible for a severe and under-reported public health burden throughout Southeast Asia. Here we use multilocus sequence typing (MLST) to characterize 74 clinical isolates from three geographic locations in the Lao PDR (Laos), and compare them with isolates described from Udon Thani, northeast Thailand. The data confirm high levels of diversity and recombination within the natural O. tsutsugamushi population, and a rate of mixed infection of ~8%. We compared the relationships and geographical structuring of the strains and populations using allele based approaches (eBURST), phylogenetic approaches, and by calculating F-statistics (FST). These analyses all point towards low levels of population differentiation between isolates from Vientiane and Udon Thani, cities which straddle the Mekong River which defines the Lao/Thai border, but with a very distinct population in Salavan, southern Laos. These data highlight how land use, as well as the movement of hosts and vectors, may impact on the epidemiology of zoonotic infections. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/26317624/The_Diversity_and_Geographical_Structure_of_Orientia_tsutsugamushi_Strains_from_Scrub_Typhus_Patients_in_Laos_ DB - PRIME DP - Unbound Medicine ER -