Tags

Type your tag names separated by a space and hit enter

The fungistatic and fungicidal effects of volatiles from metathoracic glands of soybean-attacking stink bugs (Heteroptera: Pentatomidae) on the entomopathogen Beauveria bassiana.
J Invertebr Pathol. 2015 Nov; 132:77-85.JI

Abstract

This study was initially designed to evaluate the differential susceptibility of three soybean-attacking pentatomids to the entomopathogenic fungus Beauveria bassiana in standardized bioassays. Euschistus heros (Eh) was shown to be significantly less susceptible than Chinavia ubica (Cu), whereas Dichelops melacanthus (Dm) adults were highly susceptible to fungal infections. A deeper look at the mechanisms involved in the possible role of volatiles from metathoracic glands on fungal infections was undertaken, and gland extracts from Nezara viridula (Nv), a species known for its resilience to fungal infections, were also included in the assays. Atmospheres with volatiles from pentatomids with very low-susceptibility to B. bassiana infections (Eh and Nv) had a significant effect on speed of germination as shown in counts performed up to 22h post-inoculation, by which time 0.1 (control), 0.6 (Dm), 17.9 (Cu), 32.6 (Eh), and 43.4% (Nv) of conidia had not germinated. The fungistatic (inhibitory) and fungicidal (lethal) effects of Eh and Nv volatile-rich atmospheres were subsequently quantified in Petri dishes with either PDA or PDA medium amended with carbendazim, which allowed germination rates to be determined at 18 and 48h post-inoculation, respectively. As opposed to control, Eh volatile-rich atmosphere had a clear fungistatic effect, since germination rate was only 27.4% within 18h, but reached 99.4% at 48h post-inoculation. For Nv volatile-rich atmospheres, only 15.1% of conidia germinated within 18h, and by 48h post-inoculation, approx. 18% of conidia were unviable (neither germ tubes nor intumescence), whereas in the control treatment rates were >99% at both reading times. Therefore, the gaseous phase of defensive secretions from fungus-resilient pentatomids possess a strong inhibitory effect and may display a less pronounced lethal effect on fungal germination, as was the case for Nv.

Authors+Show Affiliations

EMBRAPA Genetic Resources and Biotechnology, Parque Estação Biológica, W5 Norte (Final), 70770-917 Brasilia, DF, Brazil.EMBRAPA Genetic Resources and Biotechnology, Parque Estação Biológica, W5 Norte (Final), 70770-917 Brasilia, DF, Brazil.EMBRAPA Genetic Resources and Biotechnology, Parque Estação Biológica, W5 Norte (Final), 70770-917 Brasilia, DF, Brazil.EMBRAPA Genetic Resources and Biotechnology, Parque Estação Biológica, W5 Norte (Final), 70770-917 Brasilia, DF, Brazil.EMBRAPA Genetic Resources and Biotechnology, Parque Estação Biológica, W5 Norte (Final), 70770-917 Brasilia, DF, Brazil. Electronic address: marcos.faria@embrapa.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26321153

Citation

Lopes, Rogério B., et al. "The Fungistatic and Fungicidal Effects of Volatiles From Metathoracic Glands of Soybean-attacking Stink Bugs (Heteroptera: Pentatomidae) On the Entomopathogen Beauveria Bassiana." Journal of Invertebrate Pathology, vol. 132, 2015, pp. 77-85.
Lopes RB, Laumann RA, Blassioli-Moraes MC, et al. The fungistatic and fungicidal effects of volatiles from metathoracic glands of soybean-attacking stink bugs (Heteroptera: Pentatomidae) on the entomopathogen Beauveria bassiana. J Invertebr Pathol. 2015;132:77-85.
Lopes, R. B., Laumann, R. A., Blassioli-Moraes, M. C., Borges, M., & Faria, M. (2015). The fungistatic and fungicidal effects of volatiles from metathoracic glands of soybean-attacking stink bugs (Heteroptera: Pentatomidae) on the entomopathogen Beauveria bassiana. Journal of Invertebrate Pathology, 132, 77-85. https://doi.org/10.1016/j.jip.2015.08.011
Lopes RB, et al. The Fungistatic and Fungicidal Effects of Volatiles From Metathoracic Glands of Soybean-attacking Stink Bugs (Heteroptera: Pentatomidae) On the Entomopathogen Beauveria Bassiana. J Invertebr Pathol. 2015;132:77-85. PubMed PMID: 26321153.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The fungistatic and fungicidal effects of volatiles from metathoracic glands of soybean-attacking stink bugs (Heteroptera: Pentatomidae) on the entomopathogen Beauveria bassiana. AU - Lopes,Rogério B, AU - Laumann,Raul A, AU - Blassioli-Moraes,Maria C, AU - Borges,Miguel, AU - Faria,Marcos, Y1 - 2015/08/28/ PY - 2015/04/29/received PY - 2015/08/25/revised PY - 2015/08/26/accepted PY - 2015/9/1/entrez PY - 2015/9/1/pubmed PY - 2016/12/15/medline KW - Antimicrobial activity KW - Biological control KW - Biopesticides KW - Entomopathogenic fungi KW - Germination KW - Mycoinsecticides SP - 77 EP - 85 JF - Journal of invertebrate pathology JO - J. Invertebr. Pathol. VL - 132 N2 - This study was initially designed to evaluate the differential susceptibility of three soybean-attacking pentatomids to the entomopathogenic fungus Beauveria bassiana in standardized bioassays. Euschistus heros (Eh) was shown to be significantly less susceptible than Chinavia ubica (Cu), whereas Dichelops melacanthus (Dm) adults were highly susceptible to fungal infections. A deeper look at the mechanisms involved in the possible role of volatiles from metathoracic glands on fungal infections was undertaken, and gland extracts from Nezara viridula (Nv), a species known for its resilience to fungal infections, were also included in the assays. Atmospheres with volatiles from pentatomids with very low-susceptibility to B. bassiana infections (Eh and Nv) had a significant effect on speed of germination as shown in counts performed up to 22h post-inoculation, by which time 0.1 (control), 0.6 (Dm), 17.9 (Cu), 32.6 (Eh), and 43.4% (Nv) of conidia had not germinated. The fungistatic (inhibitory) and fungicidal (lethal) effects of Eh and Nv volatile-rich atmospheres were subsequently quantified in Petri dishes with either PDA or PDA medium amended with carbendazim, which allowed germination rates to be determined at 18 and 48h post-inoculation, respectively. As opposed to control, Eh volatile-rich atmosphere had a clear fungistatic effect, since germination rate was only 27.4% within 18h, but reached 99.4% at 48h post-inoculation. For Nv volatile-rich atmospheres, only 15.1% of conidia germinated within 18h, and by 48h post-inoculation, approx. 18% of conidia were unviable (neither germ tubes nor intumescence), whereas in the control treatment rates were >99% at both reading times. Therefore, the gaseous phase of defensive secretions from fungus-resilient pentatomids possess a strong inhibitory effect and may display a less pronounced lethal effect on fungal germination, as was the case for Nv. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/26321153/The_fungistatic_and_fungicidal_effects_of_volatiles_from_metathoracic_glands_of_soybean-attacking_stink_bugs_(Heteroptera:_Pentatomidae)_on_the_entomopathogen_Beauveria_bassiana L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(15)30010-0 DB - PRIME DP - Unbound Medicine ER -