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2-DE proteomics analysis of drought treated seedlings of Quercus ilex supports a root active strategy for metabolic adaptation in response to water shortage.
Front Plant Sci. 2015; 6:627.FP

Abstract

Holm oak is a dominant tree in the western Mediterranean region. Despite being well adapted to dry hot climate, drought is the main cause of mortality post-transplanting in reforestation programs. An active response to drought is critical for tree establishment and survival. Applying a gel-based proteomic approach, dynamic changes in root proteins of drought treated Quercus ilex subsp. Ballota [Desf.] Samp. seedlings were followed. Water stress was applied on 20 day-old holm oak plantlets by water limitation for a period of 10 and 20 days, each followed by 10 days of recovery. Stress was monitored by changes in water status, plant growth, and electrolyte leakage. Contrary to leaves, holm oak roots responded readily to water shortage at physiological level by growth inhibition, changes in water status and membrane stability. Root proteins were extracted using trichloroacetate/acetone/phenol protocol and separated by two-dimensional electrophoresis. Coomassie colloidal stained gel images were analyzed and spot intensity data subjected to multivariate statistical analysis. Selected consistent spots in three biological replicas, presenting significant changes under stress, were subjected to MALDI-TOF mass spectrometry (peptide mass fingerprinting and MS/MS). For protein identification, combined search was performed with MASCOT search engine over NCBInr Viridiplantae and Uniprot databases. Data are available via ProteomeXchange with identifier PXD002484. Identified proteins were classified into functional groups: metabolism, protein biosynthesis and proteolysis, defense against biotic stress, cellular protection against abiotic stress, intracellular transport. Several enzymes of the carbohydrate metabolism decreased in abundance in roots under drought stress while some related to ATP synthesis and secondary metabolism increased. Results point at active metabolic adjustment and mobilization of the defense system in roots to actively counteract drought stress.

Authors+Show Affiliations

Agricultural and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Cordoba Cordoba, Spain.Agricultural and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Cordoba Cordoba, Spain.Agricultural and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Cordoba Cordoba, Spain.Department of Forestry Engineering, School of Agricultural and Forestry Engineering, University of Coìrdoba, Agrifood Campus of International Excellence Coìrdoba, Spain.Computational Proteomics, Proteomics Facility, Centro Nacional de Biotecnología - CSIC Madrid, Spain.Agricultural and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Cordoba Cordoba, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26322068

Citation

Simova-Stoilova, Lyudmila P., et al. "2-DE Proteomics Analysis of Drought Treated Seedlings of Quercus Ilex Supports a Root Active Strategy for Metabolic Adaptation in Response to Water Shortage." Frontiers in Plant Science, vol. 6, 2015, p. 627.
Simova-Stoilova LP, Romero-Rodríguez MC, Sánchez-Lucas R, et al. 2-DE proteomics analysis of drought treated seedlings of Quercus ilex supports a root active strategy for metabolic adaptation in response to water shortage. Front Plant Sci. 2015;6:627.
Simova-Stoilova, L. P., Romero-Rodríguez, M. C., Sánchez-Lucas, R., Navarro-Cerrillo, R. M., Medina-Aunon, J. A., & Jorrín-Novo, J. V. (2015). 2-DE proteomics analysis of drought treated seedlings of Quercus ilex supports a root active strategy for metabolic adaptation in response to water shortage. Frontiers in Plant Science, 6, 627. https://doi.org/10.3389/fpls.2015.00627
Simova-Stoilova LP, et al. 2-DE Proteomics Analysis of Drought Treated Seedlings of Quercus Ilex Supports a Root Active Strategy for Metabolic Adaptation in Response to Water Shortage. Front Plant Sci. 2015;6:627. PubMed PMID: 26322068.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 2-DE proteomics analysis of drought treated seedlings of Quercus ilex supports a root active strategy for metabolic adaptation in response to water shortage. AU - Simova-Stoilova,Lyudmila P, AU - Romero-Rodríguez,Maria C, AU - Sánchez-Lucas,Rosa, AU - Navarro-Cerrillo,Rafael M, AU - Medina-Aunon,J Alberto, AU - Jorrín-Novo,Jesús V, Y1 - 2015/08/14/ PY - 2015/03/17/received PY - 2015/07/29/accepted PY - 2015/9/1/entrez PY - 2015/9/1/pubmed PY - 2015/9/1/medline KW - drought KW - holm oak KW - proteomics KW - recovery KW - roots SP - 627 EP - 627 JF - Frontiers in plant science JO - Front Plant Sci VL - 6 N2 - Holm oak is a dominant tree in the western Mediterranean region. Despite being well adapted to dry hot climate, drought is the main cause of mortality post-transplanting in reforestation programs. An active response to drought is critical for tree establishment and survival. Applying a gel-based proteomic approach, dynamic changes in root proteins of drought treated Quercus ilex subsp. Ballota [Desf.] Samp. seedlings were followed. Water stress was applied on 20 day-old holm oak plantlets by water limitation for a period of 10 and 20 days, each followed by 10 days of recovery. Stress was monitored by changes in water status, plant growth, and electrolyte leakage. Contrary to leaves, holm oak roots responded readily to water shortage at physiological level by growth inhibition, changes in water status and membrane stability. Root proteins were extracted using trichloroacetate/acetone/phenol protocol and separated by two-dimensional electrophoresis. Coomassie colloidal stained gel images were analyzed and spot intensity data subjected to multivariate statistical analysis. Selected consistent spots in three biological replicas, presenting significant changes under stress, were subjected to MALDI-TOF mass spectrometry (peptide mass fingerprinting and MS/MS). For protein identification, combined search was performed with MASCOT search engine over NCBInr Viridiplantae and Uniprot databases. Data are available via ProteomeXchange with identifier PXD002484. Identified proteins were classified into functional groups: metabolism, protein biosynthesis and proteolysis, defense against biotic stress, cellular protection against abiotic stress, intracellular transport. Several enzymes of the carbohydrate metabolism decreased in abundance in roots under drought stress while some related to ATP synthesis and secondary metabolism increased. Results point at active metabolic adjustment and mobilization of the defense system in roots to actively counteract drought stress. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/26322068/2_DE_proteomics_analysis_of_drought_treated_seedlings_of_Quercus_ilex_supports_a_root_active_strategy_for_metabolic_adaptation_in_response_to_water_shortage_ L2 - https://doi.org/10.3389/fpls.2015.00627 DB - PRIME DP - Unbound Medicine ER -