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Comparison of selected methods for recovery of Giardia spp. cysts and Cryptosporidium spp. oocysts in wastewater.
J Water Health. 2015 Sep; 13(3):811-8.JW

Abstract

More precise methods are needed to recover Giardia and Cryptosporidium (oo)cysts from wastewater in order to advance research related to their inactivation, removal, quantification, and species differentiation. This study applied different methods to recover the maximum number of (oo)cysts from wastewater samples using ColorSeed®. Immunomagnetic separation assisted in capturing oocysts mainly in samples with medium and low turbidity. A triple centrifugation method reached recovery rates of 85% and 20%, for Giardia cysts and Cryptosporidium oocysts, respectively, in raw wastewater, and 62.5 and 17.5% in secondary-treated effluent. For low turbidity-treated effluent, membrane filtration reached 67.5% recovery for Giardia cysts and 22.5% for Cryptosporidium oocysts. Simple, quick and low-cost methods do not involve much handling of the samples and could be useful, particularly in developing countries.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Medeiros RC
Department of Agronomic and Environmental Sciences, Federal University of Santa Maria, Linha 7 de Setembro, BR 386, Km 40, 98400-000, Frederico Westphalen - Rio Grande do Sul, Brazil E-mail: medeiroscg@yahoo.com.br.
Daniel LA
Hydraulics and Sanitation Department, São Carlos School of Engineering, University of São Paulo, Av. Trabalhador São-Carlense, 400, 13566-590, São Carlos - São Paulo, Brazil.

MeSH

CryptosporidiumFiltrationGiardiaImmunomagnetic SeparationOocystsWastewaterWater Purification

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26322766

Citation

Medeiros, Raphael Corrêa, and Luiz Antonio Daniel. "Comparison of Selected Methods for Recovery of Giardia Spp. Cysts and Cryptosporidium Spp. Oocysts in Wastewater." Journal of Water and Health, vol. 13, no. 3, 2015, pp. 811-8.
Medeiros RC, Daniel LA. Comparison of selected methods for recovery of Giardia spp. cysts and Cryptosporidium spp. oocysts in wastewater. J Water Health. 2015;13(3):811-8.
Medeiros, R. C., & Daniel, L. A. (2015). Comparison of selected methods for recovery of Giardia spp. cysts and Cryptosporidium spp. oocysts in wastewater. Journal of Water and Health, 13(3), 811-8. https://doi.org/10.2166/wh.2015.228
Medeiros RC, Daniel LA. Comparison of Selected Methods for Recovery of Giardia Spp. Cysts and Cryptosporidium Spp. Oocysts in Wastewater. J Water Health. 2015;13(3):811-8. PubMed PMID: 26322766.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of selected methods for recovery of Giardia spp. cysts and Cryptosporidium spp. oocysts in wastewater. AU - Medeiros,Raphael Corrêa, AU - Daniel,Luiz Antonio, PY - 2015/9/1/entrez PY - 2015/9/1/pubmed PY - 2015/11/7/medline SP - 811 EP - 8 JF - Journal of water and health JO - J Water Health VL - 13 IS - 3 N2 - More precise methods are needed to recover Giardia and Cryptosporidium (oo)cysts from wastewater in order to advance research related to their inactivation, removal, quantification, and species differentiation. This study applied different methods to recover the maximum number of (oo)cysts from wastewater samples using ColorSeed®. Immunomagnetic separation assisted in capturing oocysts mainly in samples with medium and low turbidity. A triple centrifugation method reached recovery rates of 85% and 20%, for Giardia cysts and Cryptosporidium oocysts, respectively, in raw wastewater, and 62.5 and 17.5% in secondary-treated effluent. For low turbidity-treated effluent, membrane filtration reached 67.5% recovery for Giardia cysts and 22.5% for Cryptosporidium oocysts. Simple, quick and low-cost methods do not involve much handling of the samples and could be useful, particularly in developing countries. SN - 1477-8920 UR - https://www.unboundmedicine.com/medline/citation/26322766/Comparison_of_selected_methods_for_recovery_of_Giardia_spp__cysts_and_Cryptosporidium_spp__oocysts_in_wastewater_ DB - PRIME DP - Unbound Medicine ER -