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Snake venomics of Micrurus alleni and Micrurus mosquitensis from the Caribbean region of Costa Rica reveals two divergent compositional patterns in New World elapids.
Toxicon. 2015 Dec 01; 107(Pt B):217-33.T

Abstract

Protein composition, toxicity, and neutralization of the venoms of Micrurus alleni and Micrurus mosquitensis, two sympatric monadal coral snakes found in humid environments of the Caribbean region of Costa Rica, were studied. Proteomic profiling revealed that these venoms display highly divergent compositions: the former dominated by three-finger toxins (3FTx) and the latter by phospholipases A2 (PLA2). Protein family abundances correlated with enzymatic and toxic characteristics of the venoms. Selective inhibition experiments showed that PLA2s play only a marginal role in the lethal effect of M. alleni venom, but have a major role in M. mosquitensis venom. Proteomic data gathered from other Micrurus species evidenced that the two divergent venom phenotypes are recurrent, and may constitute a general trend across New World elapids. Further, M. mosquitensis, but not M. alleni, venom contains PLA2-like/Kunitz-type inhibitor complex(es) that resemble the ASIC1a/2-activating MitTx heterodimeric toxin isolated from Micrurus tener venom. The evolutionary origin and adaptive relevance of the puzzling phenotypic variability of Micrurus venoms remain to be understood. An antivenom against the PLA2-predominant Micrurus nigrocinctus venom strongly cross-recognized and neutralized M. mosquitensis venom, but only weakly M. alleni venom.

Authors+Show Affiliations

Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto de Biomedicina de Valencia, CSIC, Jaume Roig 11, 46010 Valencia, Spain.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Programa de Ofidismo y Escorpionismo, Universidad de Antioquia, Medellín, Colombia.Instituto de Biomedicina de Valencia, CSIC, Jaume Roig 11, 46010 Valencia, Spain.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto de Biomedicina de Valencia, CSIC, Jaume Roig 11, 46010 Valencia, Spain.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica. Electronic address: bruno.lomonte@ucr.ac.cr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26325292

Citation

Fernández, Julián, et al. "Snake Venomics of Micrurus Alleni and Micrurus Mosquitensis From the Caribbean Region of Costa Rica Reveals Two Divergent Compositional Patterns in New World Elapids." Toxicon : Official Journal of the International Society On Toxinology, vol. 107, no. Pt B, 2015, pp. 217-33.
Fernández J, Vargas-Vargas N, Pla D, et al. Snake venomics of Micrurus alleni and Micrurus mosquitensis from the Caribbean region of Costa Rica reveals two divergent compositional patterns in New World elapids. Toxicon. 2015;107(Pt B):217-33.
Fernández, J., Vargas-Vargas, N., Pla, D., Sasa, M., Rey-Suárez, P., Sanz, L., Gutiérrez, J. M., Calvete, J. J., & Lomonte, B. (2015). Snake venomics of Micrurus alleni and Micrurus mosquitensis from the Caribbean region of Costa Rica reveals two divergent compositional patterns in New World elapids. Toxicon : Official Journal of the International Society On Toxinology, 107(Pt B), 217-33. https://doi.org/10.1016/j.toxicon.2015.08.016
Fernández J, et al. Snake Venomics of Micrurus Alleni and Micrurus Mosquitensis From the Caribbean Region of Costa Rica Reveals Two Divergent Compositional Patterns in New World Elapids. Toxicon. 2015 Dec 1;107(Pt B):217-33. PubMed PMID: 26325292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Snake venomics of Micrurus alleni and Micrurus mosquitensis from the Caribbean region of Costa Rica reveals two divergent compositional patterns in New World elapids. AU - Fernández,Julián, AU - Vargas-Vargas,Nancy, AU - Pla,Davinia, AU - Sasa,Mahmood, AU - Rey-Suárez,Paola, AU - Sanz,Libia, AU - Gutiérrez,José María, AU - Calvete,Juan J, AU - Lomonte,Bruno, Y1 - 2015/08/29/ PY - 2015/06/25/received PY - 2015/08/25/accepted PY - 2015/9/2/entrez PY - 2015/9/2/pubmed PY - 2016/10/13/medline KW - Antivenom KW - Coral snake KW - Elapidae KW - Micrurus alleni KW - Micrurus mosquitensis KW - Venom SP - 217 EP - 33 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 107 IS - Pt B N2 - Protein composition, toxicity, and neutralization of the venoms of Micrurus alleni and Micrurus mosquitensis, two sympatric monadal coral snakes found in humid environments of the Caribbean region of Costa Rica, were studied. Proteomic profiling revealed that these venoms display highly divergent compositions: the former dominated by three-finger toxins (3FTx) and the latter by phospholipases A2 (PLA2). Protein family abundances correlated with enzymatic and toxic characteristics of the venoms. Selective inhibition experiments showed that PLA2s play only a marginal role in the lethal effect of M. alleni venom, but have a major role in M. mosquitensis venom. Proteomic data gathered from other Micrurus species evidenced that the two divergent venom phenotypes are recurrent, and may constitute a general trend across New World elapids. Further, M. mosquitensis, but not M. alleni, venom contains PLA2-like/Kunitz-type inhibitor complex(es) that resemble the ASIC1a/2-activating MitTx heterodimeric toxin isolated from Micrurus tener venom. The evolutionary origin and adaptive relevance of the puzzling phenotypic variability of Micrurus venoms remain to be understood. An antivenom against the PLA2-predominant Micrurus nigrocinctus venom strongly cross-recognized and neutralized M. mosquitensis venom, but only weakly M. alleni venom. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/26325292/Snake_venomics_of_Micrurus_alleni_and_Micrurus_mosquitensis_from_the_Caribbean_region_of_Costa_Rica_reveals_two_divergent_compositional_patterns_in_New_World_elapids_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(15)30046-5 DB - PRIME DP - Unbound Medicine ER -