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In Vivo Consumption of Cranberry Exerts ex Vivo Antiadhesive Activity against FimH-Dominated Uropathogenic Escherichia coli: A Combined in Vivo, ex Vivo, and in Vitro Study of an Extract from Vaccinium macrocarpon.
J Agric Food Chem. 2015 Oct 14; 63(40):8804-18.JA

Abstract

For investigation of the molecular interaction of cranberry extract with adhesins of uropathogenic Escherichia coli (UPEC), urine from four volunteers consuming standardized cranberry extract (proanthocyanidin content = 1.24%) was analyzed within ex vivo experiments, indicating time-dependent significant inhibition of 40-50% of bacterial adhesion of UPEC strain NU14 to human T24 bladder cells. Under in vitro conditions a dose-dependent increase in bacterial adhesion was observed with proanthocyanidin-enriched cranberry Vaccinium macrocarpon extract (proanthocyanidin content = 21%). Confocal laser scanning microscopy and scanning electron microscopy proved that V.m. extract led to the formation of bacterial clusters on the outer plasma membrane of the host cells without subsequent internalization. This agglomerating activity was not observed when a PAC-depleted extract (V.m. extract(≠PAC)) was used, which showed significant inhibition of bacterial adhesion in cases where type 1 fimbriae dominated and mannose-sensitive UPEC strain NU14 was used. V.m. extract(≠PAC) had no inhibitory activity against P- and F1C-fimbriae dominated strain 2980. Quantitative gene expression analysis indicated that PAC-containing as well as PAC-depleted cranberry extracts increased the fimH expression in NU14 as part of a feedback mechanism after blocking FimH. For strain 2980 the PAC-containing extract led to up-regulation of P- and F1C-fimbriae, whereas the PAC-depleted extract had no influence on gene expression. V.m. and V.m. extract(≠PAC) did not influence biofilm and curli formation in UPEC strains NU14 and 2980. These data lead to the conclusion that also proanthocyanidin-free cranberry extracts exert antiadhesive activity by interaction with mannose-sensitive type 1 fimbriae of UPEC.

Authors+Show Affiliations

Institute of Pharmaceutical Biology and Phytochemistry, University of Münster , Corrensstrasse 48, D-48149 Münster, Germany.Institute of Pharmaceutical Biology and Phytochemistry, University of Münster , Corrensstrasse 48, D-48149 Münster, Germany.Institute of Pharmaceutical Biology and Phytochemistry, University of Münster , Corrensstrasse 48, D-48149 Münster, Germany.Institute of Hygiene, University Hospital Münster , Mendelstraβe 7, D-48149 Münster, Germany.Institute of Pharmaceutical Biology and Phytochemistry, University of Münster , Corrensstrasse 48, D-48149 Münster, Germany.

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26330108

Citation

Rafsanjany, Nasli, et al. "In Vivo Consumption of Cranberry Exerts Ex Vivo Antiadhesive Activity Against FimH-Dominated Uropathogenic Escherichia Coli: a Combined in Vivo, Ex Vivo, and in Vitro Study of an Extract From Vaccinium Macrocarpon." Journal of Agricultural and Food Chemistry, vol. 63, no. 40, 2015, pp. 8804-18.
Rafsanjany N, Senker J, Brandt S, et al. In Vivo Consumption of Cranberry Exerts ex Vivo Antiadhesive Activity against FimH-Dominated Uropathogenic Escherichia coli: A Combined in Vivo, ex Vivo, and in Vitro Study of an Extract from Vaccinium macrocarpon. J Agric Food Chem. 2015;63(40):8804-18.
Rafsanjany, N., Senker, J., Brandt, S., Dobrindt, U., & Hensel, A. (2015). In Vivo Consumption of Cranberry Exerts ex Vivo Antiadhesive Activity against FimH-Dominated Uropathogenic Escherichia coli: A Combined in Vivo, ex Vivo, and in Vitro Study of an Extract from Vaccinium macrocarpon. Journal of Agricultural and Food Chemistry, 63(40), 8804-18. https://doi.org/10.1021/acs.jafc.5b03030
Rafsanjany N, et al. In Vivo Consumption of Cranberry Exerts Ex Vivo Antiadhesive Activity Against FimH-Dominated Uropathogenic Escherichia Coli: a Combined in Vivo, Ex Vivo, and in Vitro Study of an Extract From Vaccinium Macrocarpon. J Agric Food Chem. 2015 Oct 14;63(40):8804-18. PubMed PMID: 26330108.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In Vivo Consumption of Cranberry Exerts ex Vivo Antiadhesive Activity against FimH-Dominated Uropathogenic Escherichia coli: A Combined in Vivo, ex Vivo, and in Vitro Study of an Extract from Vaccinium macrocarpon. AU - Rafsanjany,Nasli, AU - Senker,Jandirk, AU - Brandt,Simone, AU - Dobrindt,Ulrich, AU - Hensel,Andreas, Y1 - 2015/09/29/ PY - 2015/9/3/entrez PY - 2015/9/4/pubmed PY - 2016/4/23/medline KW - UPEC KW - adhesion KW - bladder cells KW - cranberry KW - fimH KW - gene expression KW - proanthocyanidins KW - uropathogenic E. coli SP - 8804 EP - 18 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 63 IS - 40 N2 - For investigation of the molecular interaction of cranberry extract with adhesins of uropathogenic Escherichia coli (UPEC), urine from four volunteers consuming standardized cranberry extract (proanthocyanidin content = 1.24%) was analyzed within ex vivo experiments, indicating time-dependent significant inhibition of 40-50% of bacterial adhesion of UPEC strain NU14 to human T24 bladder cells. Under in vitro conditions a dose-dependent increase in bacterial adhesion was observed with proanthocyanidin-enriched cranberry Vaccinium macrocarpon extract (proanthocyanidin content = 21%). Confocal laser scanning microscopy and scanning electron microscopy proved that V.m. extract led to the formation of bacterial clusters on the outer plasma membrane of the host cells without subsequent internalization. This agglomerating activity was not observed when a PAC-depleted extract (V.m. extract(≠PAC)) was used, which showed significant inhibition of bacterial adhesion in cases where type 1 fimbriae dominated and mannose-sensitive UPEC strain NU14 was used. V.m. extract(≠PAC) had no inhibitory activity against P- and F1C-fimbriae dominated strain 2980. Quantitative gene expression analysis indicated that PAC-containing as well as PAC-depleted cranberry extracts increased the fimH expression in NU14 as part of a feedback mechanism after blocking FimH. For strain 2980 the PAC-containing extract led to up-regulation of P- and F1C-fimbriae, whereas the PAC-depleted extract had no influence on gene expression. V.m. and V.m. extract(≠PAC) did not influence biofilm and curli formation in UPEC strains NU14 and 2980. These data lead to the conclusion that also proanthocyanidin-free cranberry extracts exert antiadhesive activity by interaction with mannose-sensitive type 1 fimbriae of UPEC. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/26330108/In_Vivo_Consumption_of_Cranberry_Exerts_ex_Vivo_Antiadhesive_Activity_against_FimH_Dominated_Uropathogenic_Escherichia_coli:_A_Combined_in_Vivo_ex_Vivo_and_in_Vitro_Study_of_an_Extract_from_Vaccinium_macrocarpon_ L2 - https://dx.doi.org/10.1021/acs.jafc.5b03030 DB - PRIME DP - Unbound Medicine ER -