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Topical Mitomycin-C enhances subbasal nerve regeneration and reduces erosion frequency in the debridement wounded mouse cornea.
Exp Eye Res. 2016 05; 146:361-369.EE

Abstract

Corneal epithelial basement membrane dystrophies and superficial injuries caused by scratches can lead to recurrent corneal erosion syndrome (RCES). Patients and animals with reduced corneal sensory nerve innervation can also develop recurrent erosions. Multiple wild-type mouse strains will spontaneously develop recurrent corneal erosions after single 1.5 mm debridement wounds. Here we show that this wound is accompanied by an increase in corneal epithelial cell proliferation after wound closure but without a commensurate increase in corneal epithelial thickness. We investigated whether excess corneal epithelial cell proliferation contributes to erosion formation. We found that topical application of Mitomycin C (MMC), a drug used clinically to improve healing after glaucoma and refractive surgery, reduces erosion frequency, enhances subbasal axon density to levels seen in unwounded corneas, and prevents excess epithelial cell proliferation after debridement wounding. These results suggest that topically applied MMC, which successfully reduces corneal haze and scarring after PRK, may also function to enhance subbasal nerve regeneration and epithelial adhesion when used to treat RCES.

Authors+Show Affiliations

Department of Anatomy and Regenerative Biology, The George Washington University Medical School, Washington, DC 20037, USA.Department of Anatomy and Regenerative Biology, The George Washington University Medical School, Washington, DC 20037, USA.Department of Anatomy and Regenerative Biology, The George Washington University Medical School, Washington, DC 20037, USA.Department of Anatomy and Regenerative Biology, The George Washington University Medical School, Washington, DC 20037, USA; Physiology and Neuroscience Program, University of Maryland, College Park, MD 20740, USA.Massachusetts Eye and Ear Infirmary, Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.Massachusetts Eye and Ear Infirmary, Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.Department of Anatomy and Regenerative Biology, The George Washington University Medical School, Washington, DC 20037, USA; Department of Ophthalmology, The George Washington University Medical School, Washington, DC 20037, USA. Electronic address: mastepp@gwu.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

26332224

Citation

Pal-Ghosh, Sonali, et al. "Topical Mitomycin-C Enhances Subbasal Nerve Regeneration and Reduces Erosion Frequency in the Debridement Wounded Mouse Cornea." Experimental Eye Research, vol. 146, 2016, pp. 361-369.
Pal-Ghosh S, Pajoohesh-Ganji A, Tadvalkar G, et al. Topical Mitomycin-C enhances subbasal nerve regeneration and reduces erosion frequency in the debridement wounded mouse cornea. Exp Eye Res. 2016;146:361-369.
Pal-Ghosh, S., Pajoohesh-Ganji, A., Tadvalkar, G., Kyne, B. M., Guo, X., Zieske, J. D., & Stepp, M. A. (2016). Topical Mitomycin-C enhances subbasal nerve regeneration and reduces erosion frequency in the debridement wounded mouse cornea. Experimental Eye Research, 146, 361-369. https://doi.org/10.1016/j.exer.2015.08.023
Pal-Ghosh S, et al. Topical Mitomycin-C Enhances Subbasal Nerve Regeneration and Reduces Erosion Frequency in the Debridement Wounded Mouse Cornea. Exp Eye Res. 2016;146:361-369. PubMed PMID: 26332224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Topical Mitomycin-C enhances subbasal nerve regeneration and reduces erosion frequency in the debridement wounded mouse cornea. AU - Pal-Ghosh,Sonali, AU - Pajoohesh-Ganji,Ahdeah, AU - Tadvalkar,Gauri, AU - Kyne,Briana M, AU - Guo,Xiaoqing, AU - Zieske,James D, AU - Stepp,Mary Ann, Y1 - 2015/08/30/ PY - 2015/07/21/received PY - 2015/08/25/revised PY - 2015/08/26/accepted PY - 2015/9/3/entrez PY - 2015/9/4/pubmed PY - 2017/5/26/medline KW - Cornea KW - Epithelium KW - Erosions KW - Mouse KW - Reinnervation KW - Subbasal nerves KW - Wound healing SP - 361 EP - 369 JF - Experimental eye research JO - Exp Eye Res VL - 146 N2 - Corneal epithelial basement membrane dystrophies and superficial injuries caused by scratches can lead to recurrent corneal erosion syndrome (RCES). Patients and animals with reduced corneal sensory nerve innervation can also develop recurrent erosions. Multiple wild-type mouse strains will spontaneously develop recurrent corneal erosions after single 1.5 mm debridement wounds. Here we show that this wound is accompanied by an increase in corneal epithelial cell proliferation after wound closure but without a commensurate increase in corneal epithelial thickness. We investigated whether excess corneal epithelial cell proliferation contributes to erosion formation. We found that topical application of Mitomycin C (MMC), a drug used clinically to improve healing after glaucoma and refractive surgery, reduces erosion frequency, enhances subbasal axon density to levels seen in unwounded corneas, and prevents excess epithelial cell proliferation after debridement wounding. These results suggest that topically applied MMC, which successfully reduces corneal haze and scarring after PRK, may also function to enhance subbasal nerve regeneration and epithelial adhesion when used to treat RCES. SN - 1096-0007 UR - https://www.unboundmedicine.com/medline/citation/26332224/Topical_Mitomycin_C_enhances_subbasal_nerve_regeneration_and_reduces_erosion_frequency_in_the_debridement_wounded_mouse_cornea_ DB - PRIME DP - Unbound Medicine ER -