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Potential gene regulatory role for cyclin D3 in muscle cells.
J Biosci. 2015 Sep; 40(3):497-512.JB

Abstract

Cyclin D3 is important for muscle development and regeneration, and is involved in post-mitotic arrest of muscle cells. Cyclin D3 also has cell-cycle-independent functions such as regulation of specific genes in other tissues. Ectopic expression of cyclin D3 in myoblasts, where it is normally undetectable, promotes muscle gene expression and faster differentiation kinetics upon serum depletion. In the present study, we investigated the mechanistic role of cyclin D3 in muscle gene regulation. We initially showed by mutational analysis that a stable and functional cyclin D3 was required for promoting muscle differentiation. Using chromatin immunoprecipitation assays, we demonstrated that expression of cyclin D3 in undifferentiated myoblasts altered histone epigenetic marks at promoters of muscle-specific genes like MyoD, Pax7, myogenin and muscle creatine kinase but not non-muscle genes. Cyclin D3 expression also reduced the mRNA levels of certain epigenetic modifier genes. Our data suggest that epigenetic modulation of muscle-specific genes in cyclin-D3-expressing myoblasts may be responsible for faster differentiation kinetics upon serum depletion. Our results have implications for a regulatory role for cyclin D3 in muscle-specific gene activation.

Authors+Show Affiliations

CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26333396

Citation

Athar, Fathima, and Veena K. Parnaik. "Potential Gene Regulatory Role for Cyclin D3 in Muscle Cells." Journal of Biosciences, vol. 40, no. 3, 2015, pp. 497-512.
Athar F, Parnaik VK. Potential gene regulatory role for cyclin D3 in muscle cells. J Biosci. 2015;40(3):497-512.
Athar, F., & Parnaik, V. K. (2015). Potential gene regulatory role for cyclin D3 in muscle cells. Journal of Biosciences, 40(3), 497-512.
Athar F, Parnaik VK. Potential Gene Regulatory Role for Cyclin D3 in Muscle Cells. J Biosci. 2015;40(3):497-512. PubMed PMID: 26333396.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potential gene regulatory role for cyclin D3 in muscle cells. AU - Athar,Fathima, AU - Parnaik,Veena K, PY - 2015/9/4/entrez PY - 2015/9/4/pubmed PY - 2016/6/15/medline SP - 497 EP - 512 JF - Journal of biosciences JO - J Biosci VL - 40 IS - 3 N2 - Cyclin D3 is important for muscle development and regeneration, and is involved in post-mitotic arrest of muscle cells. Cyclin D3 also has cell-cycle-independent functions such as regulation of specific genes in other tissues. Ectopic expression of cyclin D3 in myoblasts, where it is normally undetectable, promotes muscle gene expression and faster differentiation kinetics upon serum depletion. In the present study, we investigated the mechanistic role of cyclin D3 in muscle gene regulation. We initially showed by mutational analysis that a stable and functional cyclin D3 was required for promoting muscle differentiation. Using chromatin immunoprecipitation assays, we demonstrated that expression of cyclin D3 in undifferentiated myoblasts altered histone epigenetic marks at promoters of muscle-specific genes like MyoD, Pax7, myogenin and muscle creatine kinase but not non-muscle genes. Cyclin D3 expression also reduced the mRNA levels of certain epigenetic modifier genes. Our data suggest that epigenetic modulation of muscle-specific genes in cyclin-D3-expressing myoblasts may be responsible for faster differentiation kinetics upon serum depletion. Our results have implications for a regulatory role for cyclin D3 in muscle-specific gene activation. SN - 0973-7138 UR - https://www.unboundmedicine.com/medline/citation/26333396/Potential_gene_regulatory_role_for_cyclin_D3_in_muscle_cells_ L2 - https://www.ias.ac.in/article/fulltext/jbsc/040/03/0497 DB - PRIME DP - Unbound Medicine ER -