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The antibacterial, phytochemicals and antioxidants evaluation of the root extracts of Hydnora africanaThunb. used as antidysenteric in Eastern Cape Province, South Africa.
BMC Complement Altern Med. 2015 Sep 04; 15:307.BC

Abstract

BACKGROUND

To determine the anti-dysenteric, phytochemicals and antioxidative properties of the root extracts of Hydnora africana. The use of plants for the treatment of dysentery and other diseases in traditional medicine has increased on the basis of these rich traditional medicine systems. Series of pharmacological tests are recommended since the aetiology of many diseases may be due to more than one factor.

METHODS

The agar well diffusion method was used to determine the susceptibility of bacterial strains to crude extracts of the plant. The minimum inhibitory concentration was determined by the microdilution test. The presence of phytochemicals and antioxidant was also assessed using standard methods.

RESULTS

The antimicrobial activity of H. africana against all the tested organisms demonstrated a mean zone diameter of inhibition ranging from 0 to 25 mm. The MIC of the extracts ranged from 0.071 to 5.0 mg/mL. Antioxidant activity showed lower ferric reducing activities, moderate nitric oxide, moderate DPPH and higher ABTS scavenging activities of the plant. Phytochemical assay revealed the presence and equivalent quantity of alkaloids, tannins, flavonoids, saponins and phenolic acid in the extracts. The water and methanol extracts were also shown as the best solvents of extraction for the phytochemicals.

CONCLUSIONS

The methanol and acetone extracts of H. africana exhibited a significant antibacterial and antioxidant activities, suggesting the presence of either good bioactive potency or the high concentration of the active principle in the extracts which may serve as a guide for selecting bio- medicinal substances of plant origin in antidysenteric drugs.

Authors+Show Affiliations

Medicinal Plants and Economic Development Research Centre, Department of Botany, University of Fort Hare, Alice, 5700, South Africa. olubunmiwintola@yahoo.com.Medicinal Plants and Economic Development Research Centre, Department of Botany, University of Fort Hare, Alice, 5700, South Africa. Aafolayan@ufh.ac.za.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26335685

Citation

Wintola, O A., and A J. Afolayan. "The Antibacterial, Phytochemicals and Antioxidants Evaluation of the Root Extracts of Hydnora africanaThunb. Used as Antidysenteric in Eastern Cape Province, South Africa." BMC Complementary and Alternative Medicine, vol. 15, 2015, p. 307.
Wintola OA, Afolayan AJ. The antibacterial, phytochemicals and antioxidants evaluation of the root extracts of Hydnora africanaThunb. used as antidysenteric in Eastern Cape Province, South Africa. BMC Complement Altern Med. 2015;15:307.
Wintola, O. A., & Afolayan, A. J. (2015). The antibacterial, phytochemicals and antioxidants evaluation of the root extracts of Hydnora africanaThunb. used as antidysenteric in Eastern Cape Province, South Africa. BMC Complementary and Alternative Medicine, 15, 307. https://doi.org/10.1186/s12906-015-0835-9
Wintola OA, Afolayan AJ. The Antibacterial, Phytochemicals and Antioxidants Evaluation of the Root Extracts of Hydnora africanaThunb. Used as Antidysenteric in Eastern Cape Province, South Africa. BMC Complement Altern Med. 2015 Sep 4;15:307. PubMed PMID: 26335685.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The antibacterial, phytochemicals and antioxidants evaluation of the root extracts of Hydnora africanaThunb. used as antidysenteric in Eastern Cape Province, South Africa. AU - Wintola,O A, AU - Afolayan,A J, Y1 - 2015/09/04/ PY - 2015/05/21/received PY - 2015/08/26/accepted PY - 2015/9/4/entrez PY - 2015/9/4/pubmed PY - 2016/3/10/medline SP - 307 EP - 307 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 15 N2 - BACKGROUND: To determine the anti-dysenteric, phytochemicals and antioxidative properties of the root extracts of Hydnora africana. The use of plants for the treatment of dysentery and other diseases in traditional medicine has increased on the basis of these rich traditional medicine systems. Series of pharmacological tests are recommended since the aetiology of many diseases may be due to more than one factor. METHODS: The agar well diffusion method was used to determine the susceptibility of bacterial strains to crude extracts of the plant. The minimum inhibitory concentration was determined by the microdilution test. The presence of phytochemicals and antioxidant was also assessed using standard methods. RESULTS: The antimicrobial activity of H. africana against all the tested organisms demonstrated a mean zone diameter of inhibition ranging from 0 to 25 mm. The MIC of the extracts ranged from 0.071 to 5.0 mg/mL. Antioxidant activity showed lower ferric reducing activities, moderate nitric oxide, moderate DPPH and higher ABTS scavenging activities of the plant. Phytochemical assay revealed the presence and equivalent quantity of alkaloids, tannins, flavonoids, saponins and phenolic acid in the extracts. The water and methanol extracts were also shown as the best solvents of extraction for the phytochemicals. CONCLUSIONS: The methanol and acetone extracts of H. africana exhibited a significant antibacterial and antioxidant activities, suggesting the presence of either good bioactive potency or the high concentration of the active principle in the extracts which may serve as a guide for selecting bio- medicinal substances of plant origin in antidysenteric drugs. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/26335685/The_antibacterial_phytochemicals_and_antioxidants_evaluation_of_the_root_extracts_of_Hydnora_africanaThunb__used_as_antidysenteric_in_Eastern_Cape_Province_South_Africa_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-015-0835-9 DB - PRIME DP - Unbound Medicine ER -