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The influence of different peritoneal dialysis fluids on the in vitro activity of ampicillin, daptomycin, and linezolid against Enterococcus faecalis.
Eur J Clin Microbiol Infect Dis. 2015 Nov; 34(11):2257-63.EJ

Abstract

Intraperitoneal administration of antibiotics is recommended for the treatment of peritoneal dialysis-related peritonitis. However, little data are available on a possible interference between peritoneal dialysis fluids and the activity of antimicrobial agents. Thus, the present in vitro study set out to investigate the influence of different peritoneal dialysis fluids on the antimicrobial activity of ampicillin, linezolid, and daptomycin against Enterococcus faecalis. Time-kill curves in four different peritoneal dialysis fluids were performed over 24 h with four different concentrations (1 × MIC, 4 × MIC, 8 × MIC, 30 × MIC) of each antibiotic evaluated. Cation-adjusted Mueller-Hinton broth was used as the comparator solution. All four peritoneal dialysis fluids evaluated had a bacteriostatic effect on the growth of Enterococcus faecalis. Compared to the cation-adjusted Mueller-Hinton broth comparator solution, the antimicrobial activity of all antibiotics tested was reduced. For ampicillin and linezolid, no activity was found in any peritoneal dialysis fluid, regardless of the concentration. Daptomycin demonstrated dose-dependent activity in all peritoneal dialysis fluids. Bactericidal activity was observed at the highest concentrations evaluated in Dianeal® PDG4 and Extraneal®, but not in concentrations lower than 30 × MIC and not in Nutrineal® PD4 and Physioneal® 40. The antimicrobial activity of ampicillin and linezolid is limited in peritoneal dialysis fluids in vitro. Daptomycin is highly effective in peritoneal dialysis fluids and might, thus, serve as an important treatment option in peritoneal dialysis-related peritonitis. Further studies are needed to evaluate the clinical impact of the present findings.

Authors+Show Affiliations

Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.Department of Clinical Pharmacology, Medical University Vienna, Vienna, Austria.Department of Internal Medicine I, University Hospital St. Poelten, Karl Landsteiner University of Health Sciences, St. Poelten, Austria.Department of Pharmacognosy, University of Vienna, Vienna, Austria.Department of Internal Medicine I, University Hospital St. Poelten, Karl Landsteiner University of Health Sciences, St. Poelten, Austria.Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria. heinz.burgmann@meduniwien.ac.at.Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria. Department of Dermatology, Medical University of Vienna, Vienna, Austria.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26337433

Citation

Kussmann, M, et al. "The Influence of Different Peritoneal Dialysis Fluids On the in Vitro Activity of Ampicillin, Daptomycin, and Linezolid Against Enterococcus Faecalis." European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, vol. 34, no. 11, 2015, pp. 2257-63.
Kussmann M, Schuster L, Zeitlinger M, et al. The influence of different peritoneal dialysis fluids on the in vitro activity of ampicillin, daptomycin, and linezolid against Enterococcus faecalis. Eur J Clin Microbiol Infect Dis. 2015;34(11):2257-63.
Kussmann, M., Schuster, L., Zeitlinger, M., Pichler, P., Reznicek, G., Wiesholzer, M., Burgmann, H., & Poeppl, W. (2015). The influence of different peritoneal dialysis fluids on the in vitro activity of ampicillin, daptomycin, and linezolid against Enterococcus faecalis. European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 34(11), 2257-63. https://doi.org/10.1007/s10096-015-2477-8
Kussmann M, et al. The Influence of Different Peritoneal Dialysis Fluids On the in Vitro Activity of Ampicillin, Daptomycin, and Linezolid Against Enterococcus Faecalis. Eur J Clin Microbiol Infect Dis. 2015;34(11):2257-63. PubMed PMID: 26337433.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The influence of different peritoneal dialysis fluids on the in vitro activity of ampicillin, daptomycin, and linezolid against Enterococcus faecalis. AU - Kussmann,M, AU - Schuster,L, AU - Zeitlinger,M, AU - Pichler,P, AU - Reznicek,G, AU - Wiesholzer,M, AU - Burgmann,H, AU - Poeppl,W, Y1 - 2015/09/04/ PY - 2015/06/01/received PY - 2015/08/20/accepted PY - 2015/9/5/entrez PY - 2015/9/5/pubmed PY - 2016/7/28/medline SP - 2257 EP - 63 JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology JO - Eur J Clin Microbiol Infect Dis VL - 34 IS - 11 N2 - Intraperitoneal administration of antibiotics is recommended for the treatment of peritoneal dialysis-related peritonitis. However, little data are available on a possible interference between peritoneal dialysis fluids and the activity of antimicrobial agents. Thus, the present in vitro study set out to investigate the influence of different peritoneal dialysis fluids on the antimicrobial activity of ampicillin, linezolid, and daptomycin against Enterococcus faecalis. Time-kill curves in four different peritoneal dialysis fluids were performed over 24 h with four different concentrations (1 × MIC, 4 × MIC, 8 × MIC, 30 × MIC) of each antibiotic evaluated. Cation-adjusted Mueller-Hinton broth was used as the comparator solution. All four peritoneal dialysis fluids evaluated had a bacteriostatic effect on the growth of Enterococcus faecalis. Compared to the cation-adjusted Mueller-Hinton broth comparator solution, the antimicrobial activity of all antibiotics tested was reduced. For ampicillin and linezolid, no activity was found in any peritoneal dialysis fluid, regardless of the concentration. Daptomycin demonstrated dose-dependent activity in all peritoneal dialysis fluids. Bactericidal activity was observed at the highest concentrations evaluated in Dianeal® PDG4 and Extraneal®, but not in concentrations lower than 30 × MIC and not in Nutrineal® PD4 and Physioneal® 40. The antimicrobial activity of ampicillin and linezolid is limited in peritoneal dialysis fluids in vitro. Daptomycin is highly effective in peritoneal dialysis fluids and might, thus, serve as an important treatment option in peritoneal dialysis-related peritonitis. Further studies are needed to evaluate the clinical impact of the present findings. SN - 1435-4373 UR - https://www.unboundmedicine.com/medline/citation/26337433/The_influence_of_different_peritoneal_dialysis_fluids_on_the_in_vitro_activity_of_ampicillin_daptomycin_and_linezolid_against_Enterococcus_faecalis_ L2 - https://dx.doi.org/10.1007/s10096-015-2477-8 DB - PRIME DP - Unbound Medicine ER -