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Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark.
BMC Res Notes. 2015 Sep 04; 8:412.BR

Abstract

BACKGROUND

The various parts of Cresecentia cujete have some important biological activities. In folklore medicine leaves are used to treat hematomas, tumors and hypertension. Fruit decoction is used to treat diarrhea, stomachaches, cold, bronchitis, cough, asthma, and urethritis. The present study was designed to explore the anti-inflammatory and antibacterial potential of C. cujete leaves and stem bark. Anti-inflammatory activity was evaluated by in vitro human red blood cell (HRBC) membrane stabilization method and antibacterial activity by disc diffusion method.

METHODS

In vitro anti-inflammatory activity was evaluated by human red blood cell (HRBC) membrane stabilization method while in vitro antibacterial activity was evaluated using cultures of Escherichia coli and Staphylococcus aureus by disc diffusion method. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined by Folin-Ciocalteu's phenol reagent and by aluminium chloride method, respectively.

RESULTS

The crude ethanol extract (CEE) of leaves and bark (concentration of each 1.0 mg/ml) demonstrated strong membrane stabilizing activity (53.86 and 61.85% protection, respectively), whereas their chloroform fractions (CHF) revealed moderate activity (48.74 ± 0.56 and 43.55 ± 6.20 %, respectively) compared with standard aspirin (concentration 0.10 mg/ml) which showed 75.81% protection in this test. All the samples showed a dose dependent anti-inflammatory activity in HRBC membrane stabilization test. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined. Again, in in vitro antibacterial study, the extractives exhibited potent antibacterial activity.

CONCLUSION

Results from this study showed that the leaves and bark of C. cujete possessed anti-inflammatory as well as antibacterial activities indicating that the plant extract has therapeutic potential against the bacterial infection and also have effect on disease processes by causing destabilization of biological membranes.

Authors+Show Affiliations

Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. rumiraj2@yahoo.com.Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. nandita.rubd@gmail.com.Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. nusratjahannity@yahoo.com.Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. aashuva@yahoo.com.Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. laboni4@yahoo.com.Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh. ekram@ru.ac.bd.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26341395

Citation

Parvin, Mst Shahnaj, et al. "Evaluation of in Vitro Anti-inflammatory and Antibacterial Potential of Crescentia Cujete Leaves and Stem Bark." BMC Research Notes, vol. 8, 2015, p. 412.
Parvin MS, Das N, Jahan N, et al. Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark. BMC Res Notes. 2015;8:412.
Parvin, M. S., Das, N., Jahan, N., Akhter, M. A., Nahar, L., & Islam, M. E. (2015). Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark. BMC Research Notes, 8, 412. https://doi.org/10.1186/s13104-015-1384-5
Parvin MS, et al. Evaluation of in Vitro Anti-inflammatory and Antibacterial Potential of Crescentia Cujete Leaves and Stem Bark. BMC Res Notes. 2015 Sep 4;8:412. PubMed PMID: 26341395.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem bark. AU - Parvin,Mst Shahnaj, AU - Das,Nandita, AU - Jahan,Nusrat, AU - Akhter,Most Afia, AU - Nahar,Laizuman, AU - Islam,Md Ekramul, Y1 - 2015/09/04/ PY - 2014/11/21/received PY - 2015/08/24/accepted PY - 2015/9/6/entrez PY - 2015/9/6/pubmed PY - 2016/6/9/medline SP - 412 EP - 412 JF - BMC research notes JO - BMC Res Notes VL - 8 N2 - BACKGROUND: The various parts of Cresecentia cujete have some important biological activities. In folklore medicine leaves are used to treat hematomas, tumors and hypertension. Fruit decoction is used to treat diarrhea, stomachaches, cold, bronchitis, cough, asthma, and urethritis. The present study was designed to explore the anti-inflammatory and antibacterial potential of C. cujete leaves and stem bark. Anti-inflammatory activity was evaluated by in vitro human red blood cell (HRBC) membrane stabilization method and antibacterial activity by disc diffusion method. METHODS: In vitro anti-inflammatory activity was evaluated by human red blood cell (HRBC) membrane stabilization method while in vitro antibacterial activity was evaluated using cultures of Escherichia coli and Staphylococcus aureus by disc diffusion method. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined by Folin-Ciocalteu's phenol reagent and by aluminium chloride method, respectively. RESULTS: The crude ethanol extract (CEE) of leaves and bark (concentration of each 1.0 mg/ml) demonstrated strong membrane stabilizing activity (53.86 and 61.85% protection, respectively), whereas their chloroform fractions (CHF) revealed moderate activity (48.74 ± 0.56 and 43.55 ± 6.20 %, respectively) compared with standard aspirin (concentration 0.10 mg/ml) which showed 75.81% protection in this test. All the samples showed a dose dependent anti-inflammatory activity in HRBC membrane stabilization test. Total phenolic (TPC) and total flavonoid contents (TFC) of the crude extract and fractions were also determined. Again, in in vitro antibacterial study, the extractives exhibited potent antibacterial activity. CONCLUSION: Results from this study showed that the leaves and bark of C. cujete possessed anti-inflammatory as well as antibacterial activities indicating that the plant extract has therapeutic potential against the bacterial infection and also have effect on disease processes by causing destabilization of biological membranes. SN - 1756-0500 UR - https://www.unboundmedicine.com/medline/citation/26341395/Evaluation_of_in_vitro_anti_inflammatory_and_antibacterial_potential_of_Crescentia_cujete_leaves_and_stem_bark_ L2 - https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-015-1384-5 DB - PRIME DP - Unbound Medicine ER -