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Quantitative proteomic analysis of Vietnamese krait venoms: Neurotoxins are the major components in Bungarus multicinctus and phospholipases A2 in Bungarus fasciatus.
Toxicon. 2015 Dec 01; 107(Pt B):197-209.T

Abstract

Kraits are venomous snakes of genus Bungarus from family Elapidae. Krait venoms are generally neurotoxic, but toxicity strongly depends on the particular species and regional origin of snakes. We analyzed the proteomes of Vietnamese Bungarus multicinctus and Bungarus fasciatus venoms both qualitatively and quantitatively. It should be noted that no proteomic data for B. multicinctus venom existed so far. We have found that in this venom, almost half (45%) of the proteins by weight was represented by β-bungarotoxins, followed by three finger toxins (28%) and phospholipases A2 (16%), other proteins being present at the level of 1-3%. In B. fasciatus venom, phospholipase A2 was the main component (71%), followed by oxidase of l-amino acids (8%), acetylcholinesterase (5%) and metalloproteinases (4%). Unexpectedly, extremely low amount of three finger toxins (1%) was found in this venom. Interestingly, the presence of complement depleting factor was observed in both venoms. Although our data showed the presence of the same toxin families in Vietnamese krait venoms as those found earlier in the venoms of kraits from other geographic regions, their relative ratio is completely different. This concerns especially B. fasciatus venom with predominant content of phospholipases A2 and very low amount of three finger toxins.

Authors+Show Affiliations

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.Institute of Applied Materials Science, Vietnam Academy of Science and Technology, 01 Mac Dinh Chi Street, District 1, Ho Chi Minh, Viet Nam.Institute of Applied Materials Science, Vietnam Academy of Science and Technology, 01 Mac Dinh Chi Street, District 1, Ho Chi Minh, Viet Nam.Institute of Applied Materials Science, Vietnam Academy of Science and Technology, 01 Mac Dinh Chi Street, District 1, Ho Chi Minh, Viet Nam.Anokhin Research Institute of Normal Physiology, Ul. Baltiyskaya 8, 125315, Moscow, Russia.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya 16/10, Moscow 117997, Russia; The National University of Science and Technology MISiS, Leninskiy Prospekt 2, 119049, Moscow, Russia. Electronic address: utkin@mx.ibch.ru.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26341420

Citation

Ziganshin, Rustam H., et al. "Quantitative Proteomic Analysis of Vietnamese Krait Venoms: Neurotoxins Are the Major Components in Bungarus Multicinctus and Phospholipases A2 in Bungarus Fasciatus." Toxicon : Official Journal of the International Society On Toxinology, vol. 107, no. Pt B, 2015, pp. 197-209.
Ziganshin RH, Kovalchuk SI, Arapidi GP, et al. Quantitative proteomic analysis of Vietnamese krait venoms: Neurotoxins are the major components in Bungarus multicinctus and phospholipases A2 in Bungarus fasciatus. Toxicon. 2015;107(Pt B):197-209.
Ziganshin, R. H., Kovalchuk, S. I., Arapidi, G. P., Starkov, V. G., Hoang, A. N., Thi Nguyen, T. T., Nguyen, K. C., Shoibonov, B. B., Tsetlin, V. I., & Utkin, Y. N. (2015). Quantitative proteomic analysis of Vietnamese krait venoms: Neurotoxins are the major components in Bungarus multicinctus and phospholipases A2 in Bungarus fasciatus. Toxicon : Official Journal of the International Society On Toxinology, 107(Pt B), 197-209. https://doi.org/10.1016/j.toxicon.2015.08.026
Ziganshin RH, et al. Quantitative Proteomic Analysis of Vietnamese Krait Venoms: Neurotoxins Are the Major Components in Bungarus Multicinctus and Phospholipases A2 in Bungarus Fasciatus. Toxicon. 2015 Dec 1;107(Pt B):197-209. PubMed PMID: 26341420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative proteomic analysis of Vietnamese krait venoms: Neurotoxins are the major components in Bungarus multicinctus and phospholipases A2 in Bungarus fasciatus. AU - Ziganshin,Rustam H, AU - Kovalchuk,Sergey I, AU - Arapidi,Georgij P, AU - Starkov,Vladislav G, AU - Hoang,Anh Ngoc, AU - Thi Nguyen,Thao Thanh, AU - Nguyen,Khoa Cuu, AU - Shoibonov,Batozhab B, AU - Tsetlin,Victor I, AU - Utkin,Yuri N, Y1 - 2015/09/02/ PY - 2015/05/23/received PY - 2015/08/21/revised PY - 2015/08/27/accepted PY - 2015/9/6/entrez PY - 2015/9/6/pubmed PY - 2016/10/13/medline KW - Bungarus fasciatus KW - Bungarus multicinctus KW - Krait KW - Mass-spectrometry KW - Proteome KW - Snake venom SP - 197 EP - 209 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 107 IS - Pt B N2 - Kraits are venomous snakes of genus Bungarus from family Elapidae. Krait venoms are generally neurotoxic, but toxicity strongly depends on the particular species and regional origin of snakes. We analyzed the proteomes of Vietnamese Bungarus multicinctus and Bungarus fasciatus venoms both qualitatively and quantitatively. It should be noted that no proteomic data for B. multicinctus venom existed so far. We have found that in this venom, almost half (45%) of the proteins by weight was represented by β-bungarotoxins, followed by three finger toxins (28%) and phospholipases A2 (16%), other proteins being present at the level of 1-3%. In B. fasciatus venom, phospholipase A2 was the main component (71%), followed by oxidase of l-amino acids (8%), acetylcholinesterase (5%) and metalloproteinases (4%). Unexpectedly, extremely low amount of three finger toxins (1%) was found in this venom. Interestingly, the presence of complement depleting factor was observed in both venoms. Although our data showed the presence of the same toxin families in Vietnamese krait venoms as those found earlier in the venoms of kraits from other geographic regions, their relative ratio is completely different. This concerns especially B. fasciatus venom with predominant content of phospholipases A2 and very low amount of three finger toxins. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/26341420/Quantitative_proteomic_analysis_of_Vietnamese_krait_venoms:_Neurotoxins_are_the_major_components_in_Bungarus_multicinctus_and_phospholipases_A2_in_Bungarus_fasciatus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(15)30056-8 DB - PRIME DP - Unbound Medicine ER -