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Comparison of Ultrasound-Assisted Headspace Solid-Phase Microextraction and Hydrodistillation for the Identification of Major Constituents in Two Species of Hypericum.
J Chromatogr Sci. 2016 Feb; 54(2):264-70.JC

Abstract

Ultrasound-assisted headspace solid-phase microextraction (UA-HS-SPME) and hydrodistillation (HD) methods, coupled to gas chromatography-mass spectrometry (GC-MS), were used for the extraction and analysis of volatile compounds from Hypericum perforatum and Hypericum scabrum collected from two different sites in Iran. In the UA-HS-SPME method, various experimental parameters such as the type of fiber coating, sonication time, extraction time, extraction temperature and desorption time were investigated. The highest extraction efficiency was achieved by a 100-µm polydimethylsiloxane (PDMS) fiber. Consequently, 36 compounds were identified in H. perforatum and H. scabrum samples, using the UA-HS-SPME-GC-MS method, of which 14 were the same in both species. On the other hand, 57 compounds of these species were identified by the HD-GC-MS method, of which 21 were the same. The predominant constituents identified using the UA-HS-SPME method in H. perforatum included β-caryophyllene, α-pinene, γ-cadinene, α-selinene, germacrene-D, limonene and myrcene, and in H. scabrum were α-pinene, nonane, β-pinene and limonene. The common constituents identified by the HD-GC-MS method for H. perforatum involved germacrene-D, limonene, β-caryophyllene, α-pinene, β-pinene and germacrene-B, and for H. scabrum were α-pinene, β-pinene, germacrene-D, nonane, limonene and γ-cadinene. The results about the main constituents of the examined species correspond to the findings of other researchers. Additionally, comparing UA-HS-SPME-GC-MS and HD-GC-MS methods showed that the UA-HS-SPME-GC-MS method is much faster and simpler, and it requires much less sample size and lower temperature.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Department of Chemistry, Lorestan University, Khoramabad, Iran a_ghiasvand@yahoo.com.

Department of Chemistry, Lorestan University, Khoramabad, Iran.

Department of Chemistry, Lorestan University, Khoramabad, Iran.

Department of Chemistry, Lorestan University, Khoramabad, Iran.

Department of Chemistry, Lorestan University, Khoramabad, Iran.

Hassani-Moghadam E

Agricultural and Natural Resources Research Center, Khoramabad, Iran.

Department of Chemistry, Lorestan University, Khoramabad, Iran.

MeSH

DistillationGas Chromatography-Mass SpectrometryHypericumPlant ExtractsSolid Phase MicroextractionUltrasonics

Pub Type(s)

Comparative Study

Journal Article

Language

eng

PubMed ID

26341491

Citation

Ghiasvand, Alireza, et al. "Comparison of Ultrasound-Assisted Headspace Solid-Phase Microextraction and Hydrodistillation for the Identification of Major Constituents in Two Species of Hypericum." Journal of Chromatographic Science, vol. 54, no. 2, 2016, pp. 264-70.

Ghiasvand A, Shadabi S, Hajipour S, et al. Comparison of Ultrasound-Assisted Headspace Solid-Phase Microextraction and Hydrodistillation for the Identification of Major Constituents in Two Species of Hypericum. J Chromatogr Sci. 2016;54(2):264-70.

Ghiasvand, A., Shadabi, S., Hajipour, S., Nasirian, A., Borzouei, M., Hassani-Moghadam, E., & Hashemi, P. (2016). Comparison of Ultrasound-Assisted Headspace Solid-Phase Microextraction and Hydrodistillation for the Identification of Major Constituents in Two Species of Hypericum. Journal of Chromatographic Science, 54(2), 264-70. https://doi.org/10.1093/chromsci/bmv136

Ghiasvand A, et al. Comparison of Ultrasound-Assisted Headspace Solid-Phase Microextraction and Hydrodistillation for the Identification of Major Constituents in Two Species of Hypericum. J Chromatogr Sci. 2016;54(2):264-70. PubMed PMID: 26341491.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Comparison of Ultrasound-Assisted Headspace Solid-Phase Microextraction and Hydrodistillation for the Identification of Major Constituents in Two Species of Hypericum. AU - Ghiasvand,Alireza, AU - Shadabi,Shahriar, AU - Hajipour,Somayeh, AU - Nasirian,Afagh, AU - Borzouei,Mohammad, AU - Hassani-Moghadam,Esfandyar, AU - Hashemi,Payman, Y1 - 2015/09/04/ PY - 2015/02/16/received PY - 2015/9/6/entrez PY - 2015/9/6/pubmed PY - 2016/10/8/medline SP - 264 EP - 70 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 54 IS - 2 N2 - Ultrasound-assisted headspace solid-phase microextraction (UA-HS-SPME) and hydrodistillation (HD) methods, coupled to gas chromatography-mass spectrometry (GC-MS), were used for the extraction and analysis of volatile compounds from Hypericum perforatum and Hypericum scabrum collected from two different sites in Iran. In the UA-HS-SPME method, various experimental parameters such as the type of fiber coating, sonication time, extraction time, extraction temperature and desorption time were investigated. The highest extraction efficiency was achieved by a 100-µm polydimethylsiloxane (PDMS) fiber. Consequently, 36 compounds were identified in H. perforatum and H. scabrum samples, using the UA-HS-SPME-GC-MS method, of which 14 were the same in both species. On the other hand, 57 compounds of these species were identified by the HD-GC-MS method, of which 21 were the same. The predominant constituents identified using the UA-HS-SPME method in H. perforatum included β-caryophyllene, α-pinene, γ-cadinene, α-selinene, germacrene-D, limonene and myrcene, and in H. scabrum were α-pinene, nonane, β-pinene and limonene. The common constituents identified by the HD-GC-MS method for H. perforatum involved germacrene-D, limonene, β-caryophyllene, α-pinene, β-pinene and germacrene-B, and for H. scabrum were α-pinene, β-pinene, germacrene-D, nonane, limonene and γ-cadinene. The results about the main constituents of the examined species correspond to the findings of other researchers. Additionally, comparing UA-HS-SPME-GC-MS and HD-GC-MS methods showed that the UA-HS-SPME-GC-MS method is much faster and simpler, and it requires much less sample size and lower temperature. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/26341491/Comparison_of_Ultrasound_Assisted_Headspace_Solid_Phase_Microextraction_and_Hydrodistillation_for_the_Identification_of_Major_Constituents_in_Two_Species_of_Hypericum_ DB - PRIME DP - Unbound Medicine ER -