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Functional venomics of the Sri Lankan Russell's viper (Daboia russelii) and its toxinological correlations.
J Proteomics. 2015 Oct 14; 128:403-23.JP

Abstract

The venom proteome (venomics) of the Sri Lankan Daboia russelii was elucidated using 1D SDS PAGE nano-ESI-LCMS/MS shotgun proteomics. A total of 41 different venom proteins belonging to 11 different protein families were identified. The four main protein families are phospholipase A2 (PLA2, 35.0%), snaclec (SCL, 22.4%, mainly platelet aggregation inhibitors), snake venom serine proteinase (SVSP, 16.0%, mainly Factor V activating enzyme) and snake venom metalloproteinase (SVMP, 6.9%, mainly heavy chain of Factor X activating enzyme). Other protein families that account for more than 1% of the venom protein include l-amino acid oxidase (LAAO, 5.2%), Kunitz-type serine proteinase inhibitor (KSPI, 4.6%), venom nerve growth factor (VNGF. 3.5%), 5'-nucleotidase (5'NUC, 3.0%), cysteine-rich secretory protein (CRISP, 2.0%) and phosphodiesterase (PDE, 1.3%). The venom proteome is consistent with the enzymatic and toxic activities of the venom, and it correlates with the clinical manifestations of Sri Lankan D. russelii envenomation which include hemorrhage, coagulopathy, renal failure, neuro-myotoxicity and intravascular hemolysis. The venom exhibited remarkable presypnatic neurotoxicity presumably due to the action of basic PLA2 in high abundance (35.0%). Besides, SCLs, Factor X activating enzymes (SVMPs), SVSPs, and LAAOs are potential hemotoxins (50.5%), contributing to coagulopathy and hemorrhagic syndrome in Sri Lankan D. russelii envenomation.

SIGNIFICANCE

The study demonstrated the proteomic profile of the Sri Lankan Russell's viper venom, unraveling its complex composition of toxins and correlations with major toxic activities. The types, numbers, and relative abundances of toxins were reported. The venom content was dominated by the neurotoxic basic phospholipases A2 (>30% of total protein abundance) and several hemotoxic or coagulopathic protein families (approximately 50% in total). The proteome correlates with the functional and toxinological characterizations of the venom, and reflects the pathophysiological effects of envenomation by the Sri Lankan Russell's viper. The venom proteomics may serve to propel the understanding on pathogenesis and treatment strategy for envenomation by this viper in Sri Lanka. The enriched database contributed by the proteomic findings will be useful for comparing venom variations among Russell's vipers from different geographical areas.

Authors+Show Affiliations

Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. Electronic address: tanngethong@yahoo.com.sg.Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.Department of Clinical Medicine, Faculty of Medicine, University of Colombo, Sri Lanka.Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur Malaysia. Electronic address: tanchoohock@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26342672

Citation

Tan, Nget Hong, et al. "Functional Venomics of the Sri Lankan Russell's Viper (Daboia Russelii) and Its Toxinological Correlations." Journal of Proteomics, vol. 128, 2015, pp. 403-23.
Tan NH, Fung SY, Tan KY, et al. Functional venomics of the Sri Lankan Russell's viper (Daboia russelii) and its toxinological correlations. J Proteomics. 2015;128:403-23.
Tan, N. H., Fung, S. Y., Tan, K. Y., Yap, M. K., Gnanathasan, C. A., & Tan, C. H. (2015). Functional venomics of the Sri Lankan Russell's viper (Daboia russelii) and its toxinological correlations. Journal of Proteomics, 128, 403-23. https://doi.org/10.1016/j.jprot.2015.08.017
Tan NH, et al. Functional Venomics of the Sri Lankan Russell's Viper (Daboia Russelii) and Its Toxinological Correlations. J Proteomics. 2015 Oct 14;128:403-23. PubMed PMID: 26342672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional venomics of the Sri Lankan Russell's viper (Daboia russelii) and its toxinological correlations. AU - Tan,Nget Hong, AU - Fung,Shin Yee, AU - Tan,Kae Yi, AU - Yap,Michelle Khai Khun, AU - Gnanathasan,Christeine Ariaranee, AU - Tan,Choo Hock, Y1 - 2015/09/03/ PY - 2015/07/26/received PY - 2015/08/16/revised PY - 2015/08/25/accepted PY - 2015/9/7/entrez PY - 2015/9/8/pubmed PY - 2016/9/3/medline KW - Daboia russelii KW - Presynaptic neurotoxicity KW - Snaclecs KW - Sri Lankan Russell's viper KW - Venom proteome KW - Venomics SP - 403 EP - 23 JF - Journal of proteomics JO - J Proteomics VL - 128 N2 - UNLABELLED: The venom proteome (venomics) of the Sri Lankan Daboia russelii was elucidated using 1D SDS PAGE nano-ESI-LCMS/MS shotgun proteomics. A total of 41 different venom proteins belonging to 11 different protein families were identified. The four main protein families are phospholipase A2 (PLA2, 35.0%), snaclec (SCL, 22.4%, mainly platelet aggregation inhibitors), snake venom serine proteinase (SVSP, 16.0%, mainly Factor V activating enzyme) and snake venom metalloproteinase (SVMP, 6.9%, mainly heavy chain of Factor X activating enzyme). Other protein families that account for more than 1% of the venom protein include l-amino acid oxidase (LAAO, 5.2%), Kunitz-type serine proteinase inhibitor (KSPI, 4.6%), venom nerve growth factor (VNGF. 3.5%), 5'-nucleotidase (5'NUC, 3.0%), cysteine-rich secretory protein (CRISP, 2.0%) and phosphodiesterase (PDE, 1.3%). The venom proteome is consistent with the enzymatic and toxic activities of the venom, and it correlates with the clinical manifestations of Sri Lankan D. russelii envenomation which include hemorrhage, coagulopathy, renal failure, neuro-myotoxicity and intravascular hemolysis. The venom exhibited remarkable presypnatic neurotoxicity presumably due to the action of basic PLA2 in high abundance (35.0%). Besides, SCLs, Factor X activating enzymes (SVMPs), SVSPs, and LAAOs are potential hemotoxins (50.5%), contributing to coagulopathy and hemorrhagic syndrome in Sri Lankan D. russelii envenomation. SIGNIFICANCE: The study demonstrated the proteomic profile of the Sri Lankan Russell's viper venom, unraveling its complex composition of toxins and correlations with major toxic activities. The types, numbers, and relative abundances of toxins were reported. The venom content was dominated by the neurotoxic basic phospholipases A2 (>30% of total protein abundance) and several hemotoxic or coagulopathic protein families (approximately 50% in total). The proteome correlates with the functional and toxinological characterizations of the venom, and reflects the pathophysiological effects of envenomation by the Sri Lankan Russell's viper. The venom proteomics may serve to propel the understanding on pathogenesis and treatment strategy for envenomation by this viper in Sri Lanka. The enriched database contributed by the proteomic findings will be useful for comparing venom variations among Russell's vipers from different geographical areas. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/26342672/Functional_venomics_of_the_Sri_Lankan_Russell's_viper__Daboia_russelii__and_its_toxinological_correlations_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(15)30108-1 DB - PRIME DP - Unbound Medicine ER -