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Green tea polyphenols decreases uric acid level through xanthine oxidase and renal urate transporters in hyperuricemic mice.
J Ethnopharmacol. 2015 Dec 04; 175:14-20.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Green tea is a Chinese materia medica with the main functions of "inducing urination and quenching thirst". Green tea polyphenols (GTP) are generally acknowledged as the main active fraction with multiple pharmacological functions in green tea. However, the effect of GTP on hyperuricemia is not clear till now.

AIM OF STUDY

The present study was carried out to investigate the effect of GTP on serum level of uric acid in potassium oxonate (PO)-induced hyperuricemic mice, and explore the underlying mechanisms from two aspects of production and excretion of uric acid.

MATERIALS AND METHODS

PO and GTP were intragastricly administered to mice for consecutive 7 days. Serum level of uric acid, and xanthine oxidase (XOD) activity in serum and liver were examined. Simultaneously, expression of XOD protein in liver was analyzed by Western blot assay. Expressions of urate transporters including urate-anion transporter (URAT) 1, organic anion transporter (OAT) 1 and 3 in kidney were analyzed by immunohistochemistry staining method.

RESULTS

300 and 600 mg/kg GTP significantly decreased serum level of uric acid of hyperuricemic mice in a dose-dependent manner (p<0.05 or p<0.01). Besides, 300 and 600 mg/kg GTP markedly reduced XOD activity in serum and liver of hyperuricemic mice (both p<0.01). Furthermore, 300 and 600 mg/kg GTP clearly reduced XOD expression in liver, as well as reduced URAT1 expression and increased OAT1 and OAT3 expressions in kidney of hyperuricemic mice (p<0.05 or p<0.01).

CONCLUSIONS

These results demonstrated that GTP had the effect of lowering uric acid through decreasing the uric acid production and increasing uric acid excretion. Our study suggested that GTP would be a promising candidate as a novel hypouricaemic agent for further investigation.

Authors+Show Affiliations

School of Environmental and Biological Engineering, Chongqing Technology and Business University, Chongqing 400067, China; Chongqing Key Laboratory of Nature Medicine Research, Chongqing Key Lab of Catalysis & Functional Organic Molecules, Chongqing Technology and Business University, Chongqing 400067, China. Electronic address: gangch_tcm@163.com.School of Environmental and Biological Engineering, Chongqing Technology and Business University, Chongqing 400067, China; Chongqing Key Laboratory of Nature Medicine Research, Chongqing Key Lab of Catalysis & Functional Organic Molecules, Chongqing Technology and Business University, Chongqing 400067, China.Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China; State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. Electronic address: gohey@cuhk.edu.hk.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26344851

Citation

Chen, Gang, et al. "Green Tea Polyphenols Decreases Uric Acid Level Through Xanthine Oxidase and Renal Urate Transporters in Hyperuricemic Mice." Journal of Ethnopharmacology, vol. 175, 2015, pp. 14-20.
Chen G, Tan ML, Li KK, et al. Green tea polyphenols decreases uric acid level through xanthine oxidase and renal urate transporters in hyperuricemic mice. J Ethnopharmacol. 2015;175:14-20.
Chen, G., Tan, M. L., Li, K. K., Leung, P. C., & Ko, C. H. (2015). Green tea polyphenols decreases uric acid level through xanthine oxidase and renal urate transporters in hyperuricemic mice. Journal of Ethnopharmacology, 175, 14-20. https://doi.org/10.1016/j.jep.2015.08.043
Chen G, et al. Green Tea Polyphenols Decreases Uric Acid Level Through Xanthine Oxidase and Renal Urate Transporters in Hyperuricemic Mice. J Ethnopharmacol. 2015 Dec 4;175:14-20. PubMed PMID: 26344851.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Green tea polyphenols decreases uric acid level through xanthine oxidase and renal urate transporters in hyperuricemic mice. AU - Chen,Gang, AU - Tan,Ming-Liang, AU - Li,Kai-Kai, AU - Leung,Ping-Chung, AU - Ko,Chun-Hay, Y1 - 2015/09/03/ PY - 2015/05/12/received PY - 2015/07/26/revised PY - 2015/08/26/accepted PY - 2015/9/8/entrez PY - 2015/9/8/pubmed PY - 2016/10/7/medline KW - Green tea polyphenols KW - Hyperuricemia KW - Urate transporters KW - Xanthine oxidase SP - 14 EP - 20 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 175 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Green tea is a Chinese materia medica with the main functions of "inducing urination and quenching thirst". Green tea polyphenols (GTP) are generally acknowledged as the main active fraction with multiple pharmacological functions in green tea. However, the effect of GTP on hyperuricemia is not clear till now. AIM OF STUDY: The present study was carried out to investigate the effect of GTP on serum level of uric acid in potassium oxonate (PO)-induced hyperuricemic mice, and explore the underlying mechanisms from two aspects of production and excretion of uric acid. MATERIALS AND METHODS: PO and GTP were intragastricly administered to mice for consecutive 7 days. Serum level of uric acid, and xanthine oxidase (XOD) activity in serum and liver were examined. Simultaneously, expression of XOD protein in liver was analyzed by Western blot assay. Expressions of urate transporters including urate-anion transporter (URAT) 1, organic anion transporter (OAT) 1 and 3 in kidney were analyzed by immunohistochemistry staining method. RESULTS: 300 and 600 mg/kg GTP significantly decreased serum level of uric acid of hyperuricemic mice in a dose-dependent manner (p<0.05 or p<0.01). Besides, 300 and 600 mg/kg GTP markedly reduced XOD activity in serum and liver of hyperuricemic mice (both p<0.01). Furthermore, 300 and 600 mg/kg GTP clearly reduced XOD expression in liver, as well as reduced URAT1 expression and increased OAT1 and OAT3 expressions in kidney of hyperuricemic mice (p<0.05 or p<0.01). CONCLUSIONS: These results demonstrated that GTP had the effect of lowering uric acid through decreasing the uric acid production and increasing uric acid excretion. Our study suggested that GTP would be a promising candidate as a novel hypouricaemic agent for further investigation. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26344851/Green_tea_polyphenols_decreases_uric_acid_level_through_xanthine_oxidase_and_renal_urate_transporters_in_hyperuricemic_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)30112-4 DB - PRIME DP - Unbound Medicine ER -